Detailed information    

insolico Bioinformatically predicted

Overview

Name   recU   Type   Machinery gene
Locus tag   MHHHKEFG_RS09785 Genome accession   NZ_CP069125
Coordinates   1961136..1961747 (+) Length   203 a.a.
NCBI ID   WP_060596723.1    Uniprot ID   -
Organism   Bacillus pumilus strain D5     
Function   plasmid transformation; homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1956136..1966747
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MHHHKEFG_RS09745 (MHHHKEFG_01992) - 1956299..1957417 (+) 1119 WP_223250492.1 NADH-dependent flavin oxidoreductase -
  MHHHKEFG_RS09750 (MHHHKEFG_01993) - 1957471..1957977 (-) 507 WP_060596718.1 PTS glucose transporter subunit IIA -
  MHHHKEFG_RS09755 (MHHHKEFG_01994) - 1958145..1958564 (+) 420 WP_060596719.1 Hsp20/alpha crystallin family protein -
  MHHHKEFG_RS09760 (MHHHKEFG_01995) - 1958585..1959037 (-) 453 WP_060596720.1 YppG family protein -
  MHHHKEFG_RS09765 (MHHHKEFG_01996) - 1959145..1959516 (-) 372 WP_034661025.1 YppE family protein -
  MHHHKEFG_RS09770 (MHHHKEFG_01997) - 1959575..1959808 (-) 234 WP_223250487.1 DUF5446 family protein -
  MHHHKEFG_RS09775 sspM 1960005..1960109 (+) 105 WP_066030739.1 acid-soluble spore protein SspM -
  MHHHKEFG_RS09780 (MHHHKEFG_01999) - 1960133..1961089 (-) 957 WP_060596722.1 DUF2515 domain-containing protein -
  MHHHKEFG_RS09785 (MHHHKEFG_02000) recU 1961136..1961747 (+) 612 WP_060596723.1 Holliday junction resolvase RecU Machinery gene
  MHHHKEFG_RS09790 (MHHHKEFG_02001) - 1961791..1964499 (+) 2709 WP_066030738.1 PBP1A family penicillin-binding protein -
  MHHHKEFG_RS09795 (MHHHKEFG_02002) - 1964535..1965044 (-) 510 WP_034661033.1 YpoC family protein -
  MHHHKEFG_RS09800 (MHHHKEFG_02003) nth 1965041..1965703 (-) 663 WP_034661035.1 endonuclease III -
  MHHHKEFG_RS09805 (MHHHKEFG_02004) - 1965721..1966425 (-) 705 WP_034661036.1 DnaD domain-containing protein -

Sequence

Protein


Download         Length: 203 a.a.        Molecular weight: 23338.36 Da        Isoelectric Point: 8.6133

>NTDB_id=532387 MHHHKEFG_RS09785 WP_060596723.1 1961136..1961747(+) (recU) [Bacillus pumilus strain D5]
MIRYPNGKSYQPIQQIGTKKRISGESSYSNRGMTLEADLNETNQYYLVNGIAVIHKKPTPVQIVNVDYPKRSAAVIKEAY
FKQSSTTDYNGVYKGRYIDFEAKETKSTTSFPLKNFHDHQIEHMKQVEKQGGICFVIISAFGSVYYLSASDLFFFWERQQ
QNGRKSISKDELMEAGHLMTLGYSPRIDYIKVVETLHFSDESE

Nucleotide


Download         Length: 612 bp        

>NTDB_id=532387 MHHHKEFG_RS09785 WP_060596723.1 1961136..1961747(+) (recU) [Bacillus pumilus strain D5]
ATGATTCGGTATCCAAACGGAAAATCGTATCAGCCCATTCAACAAATTGGGACAAAAAAAAGAATCAGCGGCGAATCCTC
TTATAGTAACCGCGGGATGACGCTTGAGGCTGACTTAAATGAAACCAACCAGTACTATTTGGTCAACGGGATCGCAGTCA
TTCATAAAAAGCCTACCCCCGTTCAGATTGTGAATGTGGATTATCCAAAAAGAAGCGCTGCTGTTATCAAAGAAGCCTAT
TTTAAACAATCATCCACAACAGACTATAATGGAGTGTACAAAGGGCGCTATATCGATTTTGAAGCAAAGGAAACCAAAAG
CACCACCTCGTTTCCGCTCAAAAATTTTCACGATCATCAAATTGAGCATATGAAACAGGTAGAAAAGCAAGGCGGTATTT
GTTTCGTTATTATATCCGCATTTGGCTCCGTTTATTATTTGTCCGCTTCAGATCTCTTTTTCTTCTGGGAGCGGCAGCAG
CAAAACGGCCGGAAATCCATTAGCAAAGACGAATTAATGGAAGCTGGCCACTTAATGACACTTGGATATTCGCCAAGAAT
TGATTATATTAAAGTAGTGGAGACACTTCATTTTTCGGATGAAAGTGAGTAG

Domains

Predicted by InterproScan.

Recombination protein U (RecU)

(31-193)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recU Bacillus subtilis subsp. subtilis str. 168

72.772

99.507

0.724