Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   MHHHKEFG_RS08420 Genome accession   NZ_CP069125
Coordinates   1698130..1698510 (+) Length   126 a.a.
NCBI ID   WP_060596689.1    Uniprot ID   -
Organism   Bacillus pumilus strain D5     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 1693130..1703510
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MHHHKEFG_RS08410 (MHHHKEFG_01722) - 1693617..1696760 (+) 3144 WP_066030858.1 bifunctional cytochrome P450/NADPH--P450 reductase -
  MHHHKEFG_RS08415 (MHHHKEFG_01723) - 1696953..1697861 (+) 909 WP_066030857.1 trypsin-like serine peptidase -
  MHHHKEFG_RS08420 (MHHHKEFG_01724) nucA/comI 1698130..1698510 (+) 381 WP_060596689.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  MHHHKEFG_RS08425 (MHHHKEFG_01725) - 1698658..1699506 (+) 849 WP_066030856.1 STAS domain-containing protein -
  MHHHKEFG_RS08430 (MHHHKEFG_01726) - 1699540..1700082 (-) 543 WP_034660768.1 IseA DL-endopeptidase inhibitor family protein -
  MHHHKEFG_RS08435 (MHHHKEFG_01727) - 1700377..1700814 (+) 438 WP_034660769.1 hypothetical protein -
  MHHHKEFG_RS08440 (MHHHKEFG_01728) lexA 1700862..1701482 (-) 621 WP_003212311.1 transcriptional repressor LexA -
  MHHHKEFG_RS08445 (MHHHKEFG_01729) yneA 1701642..1701953 (+) 312 WP_003211955.1 cell division suppressor protein YneA -
  MHHHKEFG_RS08450 (MHHHKEFG_01730) - 1701971..1702618 (+) 648 WP_003211322.1 recombinase family protein -
  MHHHKEFG_RS08455 (MHHHKEFG_01731) - 1702688..1702918 (+) 231 WP_034660771.1 DUF896 domain-containing protein -

Sequence

Protein


Download         Length: 126 a.a.        Molecular weight: 13796.47 Da        Isoelectric Point: 8.4984

>NTDB_id=532386 MHHHKEFG_RS08420 WP_060596689.1 1698130..1698510(+) (nucA/comI) [Bacillus pumilus strain D5]
MGTLLGGFGEKHAAKGADRYDHVIQFPKERYPETGSHIQEAIRKGHSDVCTIDRNGADARRQESLKGIPTKPGFDRDEWP
MAVCLEGGKGASVQYVSPSDNRGAGSWVGHQISSYPDGKRILFIVK

Nucleotide


Download         Length: 381 bp        

>NTDB_id=532386 MHHHKEFG_RS08420 WP_060596689.1 1698130..1698510(+) (nucA/comI) [Bacillus pumilus strain D5]
ATGGGCACGTTGTTAGGTGGATTTGGGGAGAAACATGCGGCAAAAGGTGCGGATCGATATGATCATGTCATTCAATTTCC
AAAGGAACGGTACCCTGAAACAGGCAGCCATATTCAAGAAGCCATTCGAAAAGGGCATTCAGATGTGTGTACGATTGACC
GAAATGGAGCAGATGCCCGCAGGCAAGAATCATTAAAAGGAATTCCGACTAAACCTGGCTTTGACCGGGATGAGTGGCCG
ATGGCGGTTTGTCTTGAGGGAGGAAAAGGTGCGAGTGTTCAATATGTCAGCCCATCTGATAATAGGGGAGCAGGCTCATG
GGTTGGACACCAGATTAGCAGTTATCCTGATGGGAAAAGAATTTTATTTATTGTGAAATAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(29-118)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

57.983

94.444

0.548