Detailed information    

insolico Bioinformatically predicted

Overview

Name   addA   Type   Machinery gene
Locus tag   MHHHKEFG_RS05140 Genome accession   NZ_CP069125
Coordinates   1055147..1058851 (+) Length   1234 a.a.
NCBI ID   WP_066031069.1    Uniprot ID   -
Organism   Bacillus pumilus strain D5     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1050147..1063851
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MHHHKEFG_RS05125 (MHHHKEFG_01040) - 1050439..1050849 (+) 411 WP_034663477.1 DoxX family protein -
  MHHHKEFG_RS05130 (MHHHKEFG_01041) - 1051046..1051492 (-) 447 WP_034663474.1 ferritin-like domain-containing protein -
  MHHHKEFG_RS05135 (MHHHKEFG_01042) addB 1051660..1055169 (+) 3510 WP_066031070.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  MHHHKEFG_RS05140 (MHHHKEFG_01043) addA 1055147..1058851 (+) 3705 WP_066031069.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  MHHHKEFG_RS05145 (MHHHKEFG_01044) sbcD 1058878..1060053 (+) 1176 WP_060595952.1 exonuclease subunit SbcD -
  MHHHKEFG_RS05150 (MHHHKEFG_01045) sbcC 1060050..1063445 (+) 3396 WP_066031068.1 exonuclease subunit SbcC -
  MHHHKEFG_RS05155 (MHHHKEFG_01046) - 1063465..1063764 (+) 300 WP_066031067.1 hypothetical protein -

Sequence

Protein


Download         Length: 1234 a.a.        Molecular weight: 142413.46 Da        Isoelectric Point: 5.0182

>NTDB_id=532370 MHHHKEFG_RS05140 WP_066031069.1 1055147..1058851(+) (addA) [Bacillus pumilus strain D5]
MQIPKPNNSTWTDDQWEAIVSEGQDILVAAAAGSGKTAVLVERLIRKMTRPEHPVDVDRLLVVTFTNASAAEMKHRITEA
LEKELAKNPGSLHMRRQLSLMNRANISTLHSFCLQVLRTFYYEIDLDPGFRLADQTEGELLGDEVLDELFEDEYKAGKPS
FFELVDRYTSDRHDLDLQWLVKRIYEFSRSHPLPEQWMRAFLSLYDVDAQTKVEELPFYPYIKEDLSLVLRSCQELLERA
LSLSKEPDGPAPRAENFIDDLEQVNELICHQDDFEELYELLPNVNFKRLKTCKGDEYDPVLLEKATDARNQAKKQLEKLK
DEYFMRSPAQHLKSLAEMKPVVETLVDLVIQFGERFERAKQEKSIVDFSDLEHYCLRILAEQDAEGHLIETEAAKYYQQQ
FEEVLVDEYQDTNLVQETILKLVSKGEHSAEGNLFMVGDVKQSIYRFRLAEPMLFLNKYKHFQPDGKETGKRIDLNKNFR
SRSDVLDSTNFLFKQLMGETVGEIEYDEQAELKLGASYPESKDTTTEMLLVHLDQQEAETGEEREELETVQFEARIIAKK
IRELVEQPFQVYDAKQQMTRNLQYRDIVILLRSMPWAPQMMEELKKQGIPVYANLSSGYFEATEVSVILSLLKVIDNPYQ
DIPLAAVLRSPIVHLDENEMALIRTSDKKGTYYDAVKAFMSVTHSDHPTCKKLERFFQLLRKWRDFSINHSVAELIWEVY
RDTQYLDYVGGMPGGKQRQANLRALYDRAKQYEKAAFRGLFRFLRFIERMQERGDDLGAAKTFSETEDVVRMMTIHSSKG
LEFPVVFTAGLGRNFNMMDLNQSYLLDKELGFGSKYIHPELRISYATLPLVAMKKKMRKELLSEELRVLYVALTRAKEKL
FLVGSVKNQVKALSKWQNAATGEEWLLPDFERYQSKTYLDFIGPALIRHQAMSSVLEETGDVVLSHPSTFTISFTQASDL
LKEDMSLEKKQQDEVVQALMDGLPVEGYGDADEKVAERLSWKYPYLAASQVGTKQSVSEIKRMKEIQDEYSVPSSIRKAR
ATLYDRPAFMKKKTLTAAEQGTAMHTVMQHITLPSEEPYDESRIRHLLKSLQQRDLLTDEQVQSIDQEGIAAFFSTSIGQ
KLLKADWVKREVSFSMVLPVKEVYSHIDTEGEPVLIQGMIDCLFETDGKLFLLDYKTDRVQGRYTGGLDAAEPILKKRYE
TQIALYAKAVERLTNRTLEEKILYFFDGNLEISL

Nucleotide


Download         Length: 3705 bp        

>NTDB_id=532370 MHHHKEFG_RS05140 WP_066031069.1 1055147..1058851(+) (addA) [Bacillus pumilus strain D5]
ATGCAAATACCAAAACCGAATAACAGTACGTGGACGGATGACCAATGGGAAGCCATCGTTTCAGAAGGACAAGATATCTT
GGTTGCGGCGGCGGCAGGGTCAGGAAAAACAGCTGTTTTGGTTGAACGGCTGATTCGAAAAATGACCCGGCCTGAACATC
CAGTTGATGTGGACCGTCTGCTCGTTGTGACTTTCACAAATGCATCTGCGGCAGAGATGAAGCATCGGATTACAGAAGCA
CTTGAAAAAGAATTAGCCAAAAACCCTGGGTCCCTTCATATGAGAAGGCAGCTGTCCCTCATGAATCGTGCCAATATTTC
TACCTTGCACTCCTTTTGCTTGCAGGTTTTGCGCACCTTTTACTATGAAATTGATCTTGACCCAGGCTTTCGTTTGGCAG
ATCAAACAGAAGGAGAATTATTAGGAGATGAAGTGTTAGATGAACTGTTCGAGGATGAATATAAGGCTGGAAAGCCATCA
TTCTTTGAACTGGTTGATCGTTACACTTCTGACCGCCATGACTTAGATTTGCAATGGCTTGTGAAGAGAATTTATGAGTT
TTCTAGGTCTCATCCTTTACCTGAGCAATGGATGAGGGCATTCCTTTCACTTTATGATGTAGATGCACAAACAAAAGTAG
AAGAATTACCGTTTTATCCATATATCAAAGAAGATCTTTCTCTCGTTCTCCGGAGCTGCCAGGAGCTGCTTGAACGAGCT
CTTTCGCTATCAAAAGAGCCGGACGGCCCAGCACCGAGAGCAGAGAATTTTATAGATGATTTAGAGCAAGTCAATGAATT
AATCTGTCATCAAGATGATTTTGAGGAGCTTTACGAGCTTTTGCCGAACGTCAATTTTAAAAGGCTCAAAACGTGCAAAG
GGGATGAATATGACCCTGTTTTGTTAGAAAAGGCCACAGATGCACGTAATCAAGCAAAAAAACAACTAGAAAAGCTAAAA
GATGAATACTTTATGCGCAGTCCTGCCCAGCATTTAAAAAGCTTAGCTGAAATGAAGCCAGTTGTGGAGACGCTTGTAGA
TCTTGTGATCCAATTTGGTGAGCGTTTCGAGAGAGCGAAGCAGGAAAAGTCAATTGTCGACTTTTCGGATTTAGAGCACT
ATTGCTTACGCATTTTAGCGGAGCAGGATGCAGAAGGACATTTGATCGAAACAGAAGCTGCCAAGTACTATCAACAGCAG
TTTGAAGAAGTGCTCGTTGATGAATACCAGGATACAAACCTCGTACAAGAAACGATTTTAAAGCTTGTGTCTAAAGGGGA
ACATTCTGCAGAGGGCAATCTGTTTATGGTTGGTGATGTAAAGCAGTCCATTTATCGTTTTAGACTGGCTGAGCCCATGC
TCTTTTTAAACAAATATAAACACTTTCAACCAGACGGCAAAGAAACAGGGAAGAGAATTGATTTAAATAAAAACTTCCGA
AGCCGTTCAGATGTTTTAGATAGTACGAACTTTTTGTTCAAGCAGCTGATGGGGGAAACAGTTGGAGAAATTGAATATGA
TGAACAAGCTGAATTAAAGCTTGGAGCGAGTTATCCAGAAAGCAAAGATACAACGACAGAAATGCTCCTTGTCCACTTAG
ATCAGCAGGAAGCAGAAACCGGCGAAGAGCGAGAAGAACTTGAGACCGTCCAATTTGAAGCGAGAATCATTGCTAAAAAA
ATAAGAGAATTGGTGGAGCAGCCATTTCAAGTGTACGATGCAAAACAGCAAATGACCCGTAACTTGCAGTATCGTGACAT
TGTCATTTTGCTTCGGTCTATGCCGTGGGCCCCGCAAATGATGGAAGAATTAAAGAAGCAGGGAATCCCAGTATATGCGA
ATCTTTCCTCTGGTTATTTTGAAGCAACAGAGGTGTCTGTCATTCTTTCTTTGTTAAAAGTCATCGATAATCCGTATCAG
GATATTCCGCTTGCAGCTGTTTTAAGATCACCTATTGTTCATTTAGATGAAAATGAAATGGCACTTATTCGAACGAGTGA
TAAAAAAGGCACTTATTATGATGCTGTAAAGGCATTCATGAGTGTGACGCATTCTGATCACCCTACATGCAAAAAATTAG
AGCGATTTTTCCAATTGCTGCGTAAGTGGCGTGATTTCTCCATCAATCATTCGGTGGCAGAATTAATTTGGGAAGTATAC
CGAGATACGCAGTATCTTGATTATGTTGGCGGGATGCCTGGAGGTAAGCAGCGGCAGGCGAATCTTCGTGCCCTTTATGA
TCGAGCCAAGCAATATGAAAAAGCTGCCTTTAGAGGGCTATTTCGATTCCTTCGTTTTATTGAAAGAATGCAGGAGCGTG
GAGATGATCTTGGTGCGGCAAAAACGTTCAGTGAAACAGAGGACGTTGTGCGCATGATGACGATCCACAGCAGTAAAGGA
TTAGAATTTCCAGTCGTCTTTACTGCAGGACTCGGCAGGAACTTTAATATGATGGATTTAAACCAGTCCTACTTACTTGA
TAAAGAGCTAGGCTTTGGAAGCAAGTATATCCATCCAGAGCTGAGAATTAGTTATGCGACATTACCGCTTGTGGCGATGA
AAAAAAAGATGAGAAAAGAGTTATTATCTGAAGAGCTGAGGGTCTTATATGTGGCGCTCACAAGGGCTAAGGAAAAATTA
TTTCTGGTCGGCTCTGTCAAAAATCAGGTGAAAGCATTAAGCAAATGGCAAAATGCCGCGACTGGAGAAGAGTGGCTGCT
CCCTGATTTTGAACGTTATCAATCGAAAACATACTTAGATTTTATTGGCCCTGCCCTTATCCGTCATCAAGCGATGTCAT
CTGTTTTAGAAGAAACAGGCGATGTTGTTCTTTCGCATCCGTCTACATTCACTATCTCATTTACGCAAGCTTCTGATCTT
CTAAAGGAAGATATGTCACTTGAAAAGAAGCAGCAAGACGAGGTAGTACAAGCATTGATGGATGGTCTGCCTGTTGAAGG
ATATGGTGATGCTGATGAGAAGGTGGCAGAGAGACTTTCATGGAAATATCCATATCTAGCGGCCTCGCAAGTCGGCACAA
AGCAGTCTGTTTCCGAGATAAAAAGGATGAAAGAAATACAGGATGAGTACAGTGTTCCTTCTTCTATTCGAAAAGCTCGT
GCAACATTGTATGACCGGCCGGCTTTCATGAAGAAAAAAACACTCACTGCCGCAGAACAAGGCACCGCAATGCATACAGT
CATGCAGCATATCACGCTTCCTTCTGAGGAGCCCTATGATGAATCACGTATAAGGCACCTGCTCAAATCCTTACAGCAGC
GAGACTTGCTAACAGATGAGCAAGTCCAGTCAATTGATCAAGAAGGAATTGCTGCTTTCTTCTCTACTTCAATTGGTCAA
AAACTGCTGAAAGCGGACTGGGTAAAACGAGAGGTATCATTTAGTATGGTGTTACCTGTTAAAGAGGTATACAGTCATAT
TGATACTGAGGGAGAGCCTGTTCTGATTCAAGGAATGATTGACTGTTTATTTGAAACAGATGGAAAGCTATTTTTACTAG
ATTATAAAACGGATAGAGTGCAAGGCAGATATACAGGTGGACTTGACGCTGCTGAACCTATCTTGAAAAAGCGCTATGAA
ACCCAAATAGCGTTATATGCAAAAGCAGTAGAGCGTTTGACAAACAGAACATTAGAGGAGAAAATTCTCTATTTCTTCGA
TGGGAATTTAGAAATCTCTTTATAA

Domains

Predicted by InterproScan.

UvrD-like helicase C-terminal domain (UvrD_C)

(473-883)

UvrD/REP helicase N-terminal domain (UvrD-helicase)

(11-463)

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(1052-1226)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addA Bacillus subtilis subsp. subtilis str. 168

66.344

100

0.666