Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   MHHHKEFG_RS01850 Genome accession   NZ_CP069125
Coordinates   341784..342212 (-) Length   142 a.a.
NCBI ID   WP_060595509.1    Uniprot ID   -
Organism   Bacillus pumilus strain D5     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 336784..347212
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MHHHKEFG_RS01820 (MHHHKEFG_00367) - 336941..337270 (+) 330 WP_066031788.1 YckD family protein -
  MHHHKEFG_RS01825 (MHHHKEFG_00368) - 337320..338084 (-) 765 WP_060595506.1 MBL fold metallo-hydrolase -
  MHHHKEFG_RS01830 (MHHHKEFG_00369) - 338200..338616 (+) 417 WP_034664465.1 Lrp/AsnC family transcriptional regulator -
  MHHHKEFG_RS01835 (MHHHKEFG_00370) - 338645..339451 (+) 807 WP_066031346.1 aminoglycoside N(3)-acetyltransferase -
  MHHHKEFG_RS01840 (MHHHKEFG_00371) - 339453..339839 (-) 387 WP_066031345.1 competence protein ComJ -
  MHHHKEFG_RS01845 (MHHHKEFG_00372) - 339960..341657 (-) 1698 WP_066031344.1 methyl-accepting chemotaxis protein -
  MHHHKEFG_RS01850 (MHHHKEFG_00373) nucA/comI 341784..342212 (-) 429 WP_060595509.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  MHHHKEFG_RS01855 (MHHHKEFG_00375) - 342580..344649 (+) 2070 WP_066031343.1 hydantoinase/oxoprolinase family protein -
  MHHHKEFG_RS01860 (MHHHKEFG_00376) - 344639..346624 (+) 1986 WP_066031342.1 hydantoinase B/oxoprolinase family protein -

Sequence

Protein


Download         Length: 142 a.a.        Molecular weight: 15916.82 Da        Isoelectric Point: 5.5444

>NTDB_id=532361 MHHHKEFG_RS01850 WP_060595509.1 341784..342212(-) (nucA/comI) [Bacillus pumilus strain D5]
MKLLKIILLLLLIVVGVTTGYIQLEQSKQETTNSSYDKTIHFPSDRYPETAKHIEEAIDEGHSSVCTIDRKHSDEQRDQS
LHGIPTKRGYDRDEWPMAMCKEGGTGASVKYISPSDNRGAGSWVGHQLSDDPDGTRIQFIID

Nucleotide


Download         Length: 429 bp        

>NTDB_id=532361 MHHHKEFG_RS01850 WP_060595509.1 341784..342212(-) (nucA/comI) [Bacillus pumilus strain D5]
ATGAAACTTCTTAAAATCATTCTCTTGCTTTTACTAATCGTCGTTGGTGTAACAACAGGCTATATTCAGCTGGAGCAAAG
TAAACAAGAGACGACGAATTCATCTTATGACAAAACCATTCACTTCCCATCAGATCGTTACCCAGAAACCGCGAAGCACA
TAGAAGAGGCAATTGATGAAGGCCATTCATCCGTATGTACGATTGACCGTAAACATAGCGATGAACAGAGAGACCAATCA
CTTCATGGCATCCCAACAAAGCGCGGGTATGATCGGGACGAATGGCCGATGGCGATGTGTAAGGAAGGAGGAACAGGAGC
TTCTGTCAAATATATCAGTCCTTCAGATAATAGGGGAGCAGGCTCATGGGTCGGTCATCAATTATCGGATGATCCTGACG
GCACAAGAATACAATTTATCATCGATTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(46-129)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

66.116

85.211

0.563