Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   K9U13_RS00670 Genome accession   NZ_CP083478
Coordinates   149805..150245 (+) Length   146 a.a.
NCBI ID   WP_000360902.1    Uniprot ID   A7ZHJ7
Organism   Escherichia coli strain elppa10     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 144805..155245
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K9U13_RS00650 (K9U13_00650) aroP 145803..147176 (+) 1374 WP_000969914.1 aromatic amino acid transporter AroP -
  K9U13_RS00655 (K9U13_00655) ampE 147219..148073 (-) 855 WP_000172005.1 beta-lactamase regulator AmpE -
  K9U13_RS00660 (K9U13_00660) ampD 148070..148621 (-) 552 WP_000923721.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  K9U13_RS00665 (K9U13_00665) nadC 148709..149602 (+) 894 WP_001135168.1 carboxylating nicotinate-nucleotide diphosphorylase -
  K9U13_RS00670 (K9U13_00670) pilA 149805..150245 (+) 441 WP_000360902.1 prepilin peptidase-dependent pilin Machinery gene
  K9U13_RS00675 (K9U13_00675) pilB 150255..151640 (+) 1386 WP_001025155.1 type II secretion system protein GspE Machinery gene
  K9U13_RS00680 (K9U13_00680) hofC 151630..152832 (+) 1203 WP_000157234.1 protein transport protein HofC -
  K9U13_RS00685 (K9U13_00685) guaC 152867..153910 (-) 1044 WP_001217338.1 GMP reductase -
  K9U13_RS00690 (K9U13_00690) - 154066..154110 (-) 45 WP_120795372.1 protein YacM -
  K9U13_RS00695 (K9U13_00695) coaE 154135..154755 (+) 621 WP_001269520.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15630.76 Da        Isoelectric Point: 4.5559

>NTDB_id=532298 K9U13_RS00670 WP_000360902.1 149805..150245(+) (pilA) [Escherichia coli strain elppa10]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALHQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=532298 K9U13_RS00670 WP_000360902.1 149805..150245(+) (pilA) [Escherichia coli strain elppa10]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGGCTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCATC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A7ZHJ7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

41.791

91.781

0.384
  pilA Haemophilus influenzae Rd KW20

41.045

91.781

0.377