Detailed information    

insolico Bioinformatically predicted

Overview


Name   pilZ   Type   Machinery gene
Locus tag   T223_RS10550 Genome accession   NC_023066
Coordinates   2232945..2233301 (+) Length   118 a.a.
NCBI ID   WP_003091119.1    Uniprot ID   A0A0H2ZD30
Organism   Pseudomonas aeruginosa LES431     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context


Location: 2227945..2238301
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  T223_RS10525 (T223_10585) fabF 2228157..2229401 (+) 1245 WP_003104132.1 beta-ketoacyl-ACP synthase II -
  T223_RS10530 (T223_10590) pabC 2229401..2230216 (+) 816 WP_003110198.1 aminodeoxychorismate lyase -
  T223_RS10535 (T223_10595) mltG 2230222..2231271 (+) 1050 WP_012613907.1 endolytic transglycosylase MltG -
  T223_RS10540 (T223_10600) tmk 2231300..2231932 (+) 633 WP_003091125.1 dTMP kinase -
  T223_RS10545 (T223_10605) - 2231925..2232911 (+) 987 WP_003104126.1 DNA polymerase III subunit delta' -
  T223_RS10550 (T223_10610) pilZ 2232945..2233301 (+) 357 WP_003091119.1 PilZ domain-containing protein Machinery gene
  T223_RS10555 (T223_10615) - 2233316..2234092 (+) 777 WP_012613908.1 TatD family hydrolase -
  T223_RS10560 (T223_10620) - 2234318..2235451 (-) 1134 WP_012613909.1 aminotransferase class V-fold PLP-dependent enzyme -
  T223_RS10565 (T223_10625) - 2235537..2236175 (+) 639 WP_003091114.1 TetR/AcrR family transcriptional regulator -
  T223_RS10570 (T223_10630) - 2236325..2237221 (+) 897 WP_003091113.1 radical SAM protein -
  T223_RS10575 (T223_10635) - 2237286..2237918 (+) 633 WP_003091112.1 DUF4823 domain-containing protein -

Sequence


Protein


Download         Length: 118 a.a.        Molecular weight: 12903.98 Da        Isoelectric Point: 9.9969

>NTDB_id=53221 T223_RS10550 WP_003091119.1 2232945..2233301(+) (pilZ) [Pseudomonas aeruginosa LES431]
MSLPPNLGPRNGILSLTIKDKSVLYAAYMPFIRNGGLFIPTNKNYKLGDEVFMLLNLMEEPEKIPVAGKVVWITPKGAQG
NRAAGIGVQFNDGDNTARNKIETYLAGALKSDRPTHTM

Nucleotide


Download         Length: 357 bp        

>NTDB_id=53221 T223_RS10550 WP_003091119.1 2232945..2233301(+) (pilZ) [Pseudomonas aeruginosa LES431]
ATGAGTTTGCCACCCAATCTGGGGCCGCGTAACGGCATCCTGTCCTTGACCATCAAGGACAAGTCCGTGCTGTACGCCGC
CTACATGCCGTTCATCAGGAACGGCGGGCTGTTCATTCCCACCAACAAGAACTACAAGCTCGGCGACGAAGTCTTCATGC
TGCTCAACCTGATGGAAGAGCCGGAGAAGATCCCGGTGGCCGGCAAGGTCGTCTGGATCACCCCGAAGGGCGCCCAGGGC
AACCGTGCGGCTGGCATCGGCGTGCAGTTCAACGACGGTGACAACACCGCCCGCAACAAGATCGAAACCTACCTGGCCGG
GGCGCTGAAGTCGGACCGGCCGACCCACACGATGTAA

Domains


Predicted by InterProScan.

(17-106)


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure
  AlphaFold DB A0A0H2ZD30

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilZ Legionella pneumophila str. Paris

49.074

91.525

0.449

  pilZ Legionella pneumophila strain ERS1305867

49.074

91.525

0.449


Multiple sequence alignment