Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   LAJ61_RS00240 Genome accession   NZ_CP083409
Coordinates   59886..60698 (+) Length   270 a.a.
NCBI ID   WP_224054018.1    Uniprot ID   -
Organism   Moraxella osloensis strain FLMOS1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 54886..65698
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LAJ61_RS00225 (LAJ61_00220) - 55901..56539 (-) 639 WP_050325417.1 peroxiredoxin -
  LAJ61_RS00230 (LAJ61_00225) dusB 56729..57727 (-) 999 WP_224054016.1 tRNA dihydrouridine synthase DusB -
  LAJ61_RS00235 (LAJ61_00230) rlmN 58325..59524 (+) 1200 WP_224054017.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  LAJ61_RS00240 (LAJ61_00235) pilF 59886..60698 (+) 813 WP_224054018.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  LAJ61_RS00245 (LAJ61_00240) - 60700..61608 (+) 909 WP_099808110.1 helix-turn-helix domain-containing protein -
  LAJ61_RS00250 (LAJ61_00245) ispG 61664..62779 (+) 1116 WP_224054019.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  LAJ61_RS00255 (LAJ61_00250) hisS 62828..64126 (+) 1299 WP_224054020.1 histidine--tRNA ligase -
  LAJ61_RS00260 (LAJ61_00255) - 64192..65031 (+) 840 WP_224054021.1 tetratricopeptide repeat protein -

Sequence

Protein


Download         Length: 270 a.a.        Molecular weight: 29692.85 Da        Isoelectric Point: 9.6336

>NTDB_id=532071 LAJ61_RS00240 WP_224054018.1 59886..60698(+) (pilF) [Moraxella osloensis strain FLMOS1]
MIGLSSFTLCLTGCTTTRQVTTQTVTASDPSRWEKNPEEIARIRTAIAAEYIRGGKLDDAKRQLESALKSYPKSAEANDM
MGVLLQQEGSPRNMQKAEAYFKNAIALNPDFSQANNNYGVYLAQLSRNQEAIAQFQIAGSALGYDGRASALENLGRTALK
VKNKPLAIQSFIKALDANRQSIVARTELIDILLADGKFLEAQALYNDLVKIIGQSNLDPRTLSQGIRLAKLANNTIEVQK
LAQQLLDRYPLSDEAKQIRSGLLTPTSTWK

Nucleotide


Download         Length: 813 bp        

>NTDB_id=532071 LAJ61_RS00240 WP_224054018.1 59886..60698(+) (pilF) [Moraxella osloensis strain FLMOS1]
ATGATTGGCCTTTCAAGCTTCACCCTTTGCCTAACGGGTTGTACCACCACTCGACAAGTCACCACGCAGACAGTCACCGC
CAGTGACCCAAGTCGTTGGGAAAAAAATCCAGAAGAAATCGCCAGAATTCGCACCGCGATTGCCGCTGAATATATTCGTG
GTGGAAAATTAGACGATGCCAAACGGCAGTTAGAAAGTGCATTAAAGTCTTATCCAAAGTCAGCAGAAGCCAATGATATG
ATGGGTGTGTTATTGCAGCAAGAAGGCAGCCCGCGCAATATGCAAAAAGCCGAAGCTTATTTTAAAAACGCCATCGCACT
CAATCCTGACTTTAGTCAAGCAAACAACAACTATGGCGTCTATCTCGCCCAACTCAGTCGTAACCAAGAAGCGATTGCCC
AGTTTCAAATTGCGGGTTCTGCCTTAGGATATGACGGTCGTGCCAGTGCGTTAGAGAATTTAGGTCGTACAGCATTAAAG
GTCAAAAACAAACCGCTGGCGATACAATCATTTATCAAGGCGCTCGATGCCAACCGCCAAAGCATCGTCGCCCGTACAGA
GCTTATTGATATTTTATTGGCAGATGGCAAATTTTTGGAGGCGCAGGCACTGTATAATGATTTGGTTAAAATTATTGGTC
AATCCAACCTTGACCCGCGCACTTTATCACAAGGGATTAGGCTTGCCAAACTTGCGAACAATACCATAGAAGTGCAAAAA
TTAGCGCAGCAGCTTTTGGATCGCTATCCACTTAGCGATGAAGCAAAGCAGATTCGTTCTGGACTTTTAACCCCAACGTC
AACTTGGAAATAA

Domains

Predicted by InterProScan.

Tetratricopeptide repeat (TPR_16)

(93-137)

Tetratricopeptide repeat (TPR_16)

(48-79)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

42.642

98.148

0.419
  pilF Acinetobacter baylyi ADP1

43.13

97.037

0.419