Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   JN403_RS00145 Genome accession   NZ_CP068986
Coordinates   34823..36193 (-) Length   456 a.a.
NCBI ID   WP_205484751.1    Uniprot ID   -
Organism   Pseudomonas sp. 15A4     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 29823..41193
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JN403_RS00120 (JN403_00120) - 30553..30750 (-) 198 WP_065987541.1 YbdD/YjiX family protein -
  JN403_RS00125 (JN403_00125) - 30777..32846 (-) 2070 WP_205484750.1 carbon starvation CstA family protein -
  JN403_RS00130 (JN403_00130) - 33000..33368 (+) 369 WP_122536689.1 PilZ domain-containing protein -
  JN403_RS00135 (JN403_00135) - 33409..33990 (-) 582 WP_122536688.1 hypothetical protein -
  JN403_RS00140 (JN403_00140) - 34007..34615 (-) 609 WP_122536687.1 hypothetical protein -
  JN403_RS00145 (JN403_00145) radA 34823..36193 (-) 1371 WP_205484751.1 DNA repair protein RadA Machinery gene
  JN403_RS00150 (JN403_00150) mscL 36393..36842 (+) 450 WP_205484752.1 large-conductance mechanosensitive channel protein MscL -
  JN403_RS00155 (JN403_00155) - 36984..37760 (-) 777 WP_122536684.1 ferredoxin--NADP reductase -
  JN403_RS00160 (JN403_00160) - 38080..38328 (+) 249 WP_205484753.1 helix-turn-helix transcriptional regulator -
  JN403_RS00165 (JN403_00165) - 38475..39599 (+) 1125 WP_205484754.1 class I SAM-dependent methyltransferase -
  JN403_RS00170 (JN403_00170) - 39680..39880 (-) 201 WP_122536681.1 DUF2474 domain-containing protein -
  JN403_RS00175 (JN403_00175) cydB 39938..40945 (-) 1008 WP_122536680.1 cytochrome d ubiquinol oxidase subunit II -

Sequence

Protein


Download         Length: 456 a.a.        Molecular weight: 48887.25 Da        Isoelectric Point: 6.4007

>NTDB_id=531789 JN403_RS00145 WP_205484751.1 34823..36193(-) (radA) [Pseudomonas sp. 15A4]
MAKAKRMYGCTECGSTFPKWAGQCSECGAWNTLVETVIDNSTAAAPSGRSGWTGQQAQIKTLAEVSVEEIPRFSTNSTEL
DRVLGGGLVDGSVVLIGGDPGIGKSTILLQTLCAIAERMPALYVTGEESQQQVAMRARRLGLPQEKLRVMTETCIETIIA
TARIEQPKVMVIDSIQTIFTEQLQSAPGGVSQVRESAALLVRYAKQSGTAIFLVGHVTKEGALAGPRVLEHMVDTVLYFE
GESDGRLRLLRAVKNRFGAVNELGVFGMTDKGLKEVSNPSAIFLTRAQEEVAGSVVMATWEGTRPMLVEVQALVDDSHLS
NPRRVTLGLDQNRLAMLLAVLHRHGGIPTHDQDVFLNVVGGVKVLETASDLALMAAVMSSLRNRALPGDLLVFGEVGLSG
EVRPVPSGQERLKEAAKHGFKRAIVPKGNAPKESPPGLTVIGVTRLDQALDALFEE

Nucleotide


Download         Length: 1371 bp        

>NTDB_id=531789 JN403_RS00145 WP_205484751.1 34823..36193(-) (radA) [Pseudomonas sp. 15A4]
ATGGCCAAGGCCAAACGCATGTACGGCTGCACCGAGTGCGGTTCGACGTTTCCCAAGTGGGCCGGCCAGTGCAGCGAATG
CGGCGCGTGGAATACCCTCGTTGAAACCGTGATCGACAACAGCACGGCAGCGGCGCCCAGCGGTCGTTCCGGCTGGACCG
GCCAACAGGCCCAGATCAAAACCCTGGCCGAAGTCAGTGTCGAAGAAATCCCGCGCTTTTCCACCAACTCCACTGAGCTG
GACCGGGTTCTCGGCGGCGGCCTGGTGGATGGCTCGGTGGTGTTGATCGGCGGCGACCCCGGCATCGGCAAATCGACCAT
TCTGTTGCAGACCCTCTGCGCCATCGCCGAGCGCATGCCGGCGCTGTATGTCACCGGCGAAGAGTCGCAGCAGCAGGTGG
CGATGCGTGCCCGTCGGCTTGGCCTGCCCCAGGAAAAACTGCGGGTCATGACCGAGACCTGCATCGAAACCATCATCGCC
ACGGCGCGCATCGAGCAGCCTAAAGTCATGGTGATCGATTCGATCCAGACCATCTTCACCGAACAGCTGCAGTCAGCACC
TGGCGGTGTATCCCAGGTTCGCGAGAGCGCGGCGTTGCTGGTGCGCTACGCCAAGCAGAGCGGCACCGCGATCTTTCTCG
TCGGCCACGTCACCAAAGAAGGCGCGCTGGCCGGGCCTCGCGTGCTGGAGCACATGGTCGACACCGTGTTGTATTTCGAA
GGCGAATCCGATGGCCGCCTGCGCCTGCTGCGTGCGGTCAAAAACCGTTTTGGTGCCGTTAACGAACTGGGCGTGTTCGG
CATGACCGACAAAGGCCTGAAAGAAGTCTCGAATCCCTCGGCGATCTTTCTCACACGCGCGCAGGAAGAGGTCGCGGGCA
GTGTGGTCATGGCCACGTGGGAAGGCACCCGACCGATGCTGGTCGAAGTGCAGGCGCTGGTCGATGACAGCCACCTCTCC
AATCCGCGACGCGTGACGTTAGGTCTGGATCAAAACCGGCTGGCCATGTTGCTCGCGGTACTGCACCGCCACGGCGGTAT
CCCTACCCATGACCAGGATGTGTTTCTCAACGTCGTGGGCGGGGTGAAAGTGCTGGAAACCGCGTCCGACCTGGCCTTGA
TGGCCGCGGTCATGTCCAGCCTGCGTAACCGCGCGCTGCCGGGAGACTTGCTGGTGTTTGGCGAGGTGGGCTTGTCCGGC
GAGGTGCGCCCCGTCCCCAGCGGTCAGGAGCGACTCAAGGAAGCGGCCAAGCACGGCTTCAAACGCGCCATCGTGCCCAA
GGGCAACGCCCCGAAAGAGTCCCCGCCGGGCCTGACAGTGATTGGCGTGACTCGCCTGGATCAAGCTCTGGATGCGTTAT
TCGAGGAGTAA

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(353-429)

AAA domain (AAA_25)

(73-221)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

49.234

100

0.493
  radA Streptococcus pneumoniae Rx1

46.623

100

0.469
  radA Streptococcus pneumoniae R6

46.623

100

0.469
  radA Streptococcus pneumoniae TIGR4

46.623

100

0.469
  radA Streptococcus pneumoniae D39

46.623

100

0.469
  radA Streptococcus mitis SK321

46.813

99.781

0.467
  radA Streptococcus mitis NCTC 12261

46.593

99.781

0.465