Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   JNE32_RS14295 Genome accession   NZ_CP068982
Coordinates   2696416..2696826 (-) Length   136 a.a.
NCBI ID   WP_009967785.1    Uniprot ID   A0A6I4D881
Organism   Bacillus subtilis strain pb2441     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2691416..2701826
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JNE32_RS14265 (JNE32_14200) - 2691539..2691679 (+) 141 WP_003226124.1 sporulation histidine kinase inhibitor Sda -
  JNE32_RS14270 (JNE32_14205) yqeF 2691985..2692716 (-) 732 WP_029726702.1 SGNH/GDSL hydrolase family protein -
  JNE32_RS14275 (JNE32_14210) cwlH 2692968..2693720 (-) 753 WP_029726701.1 N-acetylmuramoyl-L-alanine amidase CwlH -
  JNE32_RS14280 (JNE32_14215) yqeD 2693907..2694532 (+) 626 Protein_2775 TVP38/TMEM64 family protein -
  JNE32_RS14285 (JNE32_14220) gnd 2694551..2695419 (-) 869 Protein_2776 phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) -
  JNE32_RS14290 (JNE32_14225) yqeB 2695661..2696383 (+) 723 WP_010886572.1 hypothetical protein -
  JNE32_RS14295 (JNE32_14230) nucA/comI 2696416..2696826 (-) 411 WP_009967785.1 sporulation-specific Dnase NucB Machinery gene
  JNE32_RS14300 (JNE32_14235) - 2697022..2697369 (+) 348 Protein_2779 sigma-70 family RNA polymerase sigma factor -
  JNE32_RS14305 (JNE32_14240) spoIVCA 2697400..2698860 (-) 1461 WP_223257626.1 site-specific DNA recombinase SpoIVCA -
  JNE32_RS14310 - 2698818..2698996 (-) 179 Protein_2781 hypothetical protein -
  JNE32_RS14315 (JNE32_14245) arsC 2699351..2699770 (-) 420 WP_004398596.1 thioredoxin-dependent arsenate reductase -
  JNE32_RS14320 (JNE32_14250) acr3 2699782..2700822 (-) 1041 WP_004398718.1 arsenite efflux transporter Acr3 -
  JNE32_RS14325 (JNE32_14255) arsK 2700845..2701285 (-) 441 WP_003229954.1 ArsI/CadI family heavy metal resistance metalloenzyme -
  JNE32_RS14330 (JNE32_14260) arsR 2701346..2701663 (-) 318 WP_004399122.1 arsenical resistance operon transcriptional regulator ArsR -

Sequence

Protein


Download         Length: 136 a.a.        Molecular weight: 14967.97 Da        Isoelectric Point: 5.1853

>NTDB_id=531746 JNE32_RS14295 WP_009967785.1 2696416..2696826(-) (nucA/comI) [Bacillus subtilis strain pb2441]
MKKWMAGLFLAAAVLLCLMVPQQIQGASSYDKVLYFPLSRYPETGSHIRDAIAEGHPDICTIDRDGADKRREESLKGIPT
KPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSSYPDGTRVLFIVQ

Nucleotide


Download         Length: 411 bp        

>NTDB_id=531746 JNE32_RS14295 WP_009967785.1 2696416..2696826(-) (nucA/comI) [Bacillus subtilis strain pb2441]
ATGAAAAAATGGATGGCAGGCCTGTTTCTTGCTGCAGCAGTTCTTCTTTGTTTAATGGTTCCGCAGCAGATCCAAGGCGC
ATCTTCGTATGACAAAGTGTTATATTTTCCGCTGTCTCGTTATCCGGAAACCGGCAGTCATATTAGGGATGCGATTGCAG
AGGGACATCCAGATATTTGTACCATTGATAGAGATGGAGCAGACAAAAGGCGGGAGGAATCTTTAAAGGGAATCCCGACG
AAGCCGGGCTATGACCGGGATGAGTGGCCGATGGCTGTCTGCGAGGAAGGCGGCGCAGGGGCTGATGTCCGATATGTGAC
GCCTTCTGATAATCGCGGCGCCGGCTCGTGGGTAGGGAATCAAATGAGCAGCTATCCTGACGGCACCAGAGTGCTGTTTA
TTGTGCAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(40-130)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6I4D881

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

62.609

84.559

0.529