Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   JNE32_RS14215 Genome accession   NZ_CP068982
Coordinates   2684578..2685195 (-) Length   205 a.a.
NCBI ID   WP_004398514.1    Uniprot ID   A0AAE2SLR2
Organism   Bacillus subtilis strain pb2441     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2679578..2690195
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JNE32_RS14185 (JNE32_14120) rpsT 2679880..2680146 (+) 267 WP_003229989.1 30S ribosomal protein S20 -
  JNE32_RS14190 (JNE32_14125) holA 2680161..2681204 (-) 1044 WP_003229987.1 DNA polymerase III subunit delta -
  JNE32_RS14195 (JNE32_14130) - 2681244..2681393 (-) 150 WP_003229985.1 hypothetical protein -
  JNE32_RS14200 (JNE32_14135) yqzM 2681434..2681568 (+) 135 WP_003229983.1 YqzM family protein -
  JNE32_RS14205 (JNE32_14140) comEC 2681608..2683938 (-) 2331 WP_009967776.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  JNE32_RS14210 (JNE32_14145) comEB 2683942..2684511 (-) 570 WP_003229978.1 ComE operon protein 2 -
  JNE32_RS14215 (JNE32_14150) comEA 2684578..2685195 (-) 618 WP_004398514.1 competence protein ComEA Machinery gene
  JNE32_RS14220 (JNE32_14155) comER 2685279..2686100 (+) 822 WP_004398597.1 late competence protein ComER -
  JNE32_RS14225 (JNE32_14160) yqeM 2686166..2686909 (-) 744 WP_003229973.1 class I SAM-dependent methyltransferase -
  JNE32_RS14230 (JNE32_14165) rsfS 2686906..2687262 (-) 357 WP_003229971.1 ribosome silencing factor -
  JNE32_RS14235 (JNE32_14170) yqeK 2687280..2687840 (-) 561 WP_004399059.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  JNE32_RS14240 (JNE32_14175) nadD 2687830..2688399 (-) 570 WP_004398676.1 nicotinate-nucleotide adenylyltransferase -
  JNE32_RS14245 (JNE32_14180) yhbY 2688411..2688701 (-) 291 WP_003226133.1 ribosome assembly RNA-binding protein YhbY -
  JNE32_RS14250 (JNE32_14185) aroE 2688695..2689537 (-) 843 WP_003229967.1 shikimate dehydrogenase -

Sequence

Protein


Download         Length: 205 a.a.        Molecular weight: 21769.45 Da        Isoelectric Point: 4.7220

>NTDB_id=531745 JNE32_RS14215 WP_004398514.1 2684578..2685195(-) (comEA) [Bacillus subtilis strain pb2441]
MNWLNQHKKAIILAASAAVFTAIMIFLATGKNKEPVKQAVPTETENTVVKQEANNDESNETIVIDIKGAVQHPGVYEMRT
GDRVSQAIEKAGGTSEQADEAQVNLAEILQDGTVVYIPKKGEETAVQQGGGGSVQSDGGKGALVNINTATLEELQGISGV
GPSKAEAIIAYREENGRFQTIEDITKVSGIGEKSFEKIKSSITVK

Nucleotide


Download         Length: 618 bp        

>NTDB_id=531745 JNE32_RS14215 WP_004398514.1 2684578..2685195(-) (comEA) [Bacillus subtilis strain pb2441]
ATGAATTGGTTGAATCAGCATAAGAAAGCAATTATTTTAGCGGCTTCTGCGGCTGTTTTCACAGCGATTATGATCTTTCT
GGCCACAGGGAAAAATAAAGAGCCGGTGAAGCAAGCTGTACCAACAGAGACAGAAAATACAGTGGTAAAGCAGGAAGCAA
ACAACGACGAGTCAAACGAAACAATTGTGATAGACATCAAAGGTGCTGTTCAGCATCCTGGCGTTTATGAAATGCGAACA
GGGGACAGAGTATCTCAGGCAATTGAGAAAGCGGGCGGGACCAGTGAACAAGCAGACGAAGCGCAAGTAAATTTGGCGGA
GATTCTGCAGGACGGGACAGTGGTGTACATCCCGAAAAAGGGAGAGGAAACAGCAGTGCAGCAAGGTGGCGGAGGGTCTG
TCCAAAGCGATGGAGGGAAGGGAGCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGCATCTCAGGGGTG
GGGCCATCCAAAGCTGAAGCTATTATTGCATACCGGGAGGAAAACGGTCGTTTCCAAACAATTGAAGATATCACTAAGGT
TTCAGGAATAGGTGAAAAGTCATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(142-203)

SLBB domain (SLBB)

(63-118)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

100

100

1
  comEA Staphylococcus aureus MW2

37.273

100

0.4
  comEA Staphylococcus aureus N315

36.364

100

0.39