Detailed information    

insolico Bioinformatically predicted

Overview

Name   recO   Type   Machinery gene
Locus tag   JNE32_RS14045 Genome accession   NZ_CP068982
Coordinates   2653011..2653778 (-) Length   255 a.a.
NCBI ID   WP_003230055.1    Uniprot ID   A0AAE2SJ05
Organism   Bacillus subtilis strain pb2441     
Function   ssDNA annealing; plasmid transformation (predicted from homology)   
DNA processing Homologous recombination

Genomic Context

Location: 2648011..2658778
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JNE32_RS14025 (JNE32_13960) ppsR 2648185..2648997 (-) 813 WP_003230064.1 pyruvate, water dikinase regulatory protein -
  JNE32_RS14030 (JNE32_13965) ccpN 2649023..2649661 (-) 639 WP_003237058.1 transcriptional regulator CcpN -
  JNE32_RS14035 (JNE32_13970) glyS 2649794..2651833 (-) 2040 WP_029726706.1 glycine--tRNA ligase subunit beta -
  JNE32_RS14040 (JNE32_13975) glyQ 2651826..2652713 (-) 888 WP_003226213.1 glycine--tRNA ligase subunit alpha -
  JNE32_RS14045 (JNE32_13980) recO 2653011..2653778 (-) 768 WP_003230055.1 DNA repair protein RecO Machinery gene
  JNE32_RS14050 (JNE32_13985) yqzL 2653815..2653958 (-) 144 WP_003230053.1 YqzL family protein -
  JNE32_RS14055 (JNE32_13990) era 2654106..2655011 (-) 906 WP_003230051.1 GTPase Era -
  JNE32_RS14060 (JNE32_13995) - 2654992..2655402 (-) 411 WP_003230049.1 cytidine deaminase -
  JNE32_RS14065 (JNE32_14000) unpK 2655521..2655892 (-) 372 WP_004398824.1 diacylglycerol kinase family protein -
  JNE32_RS14070 (JNE32_14005) ybeY 2655873..2656346 (-) 474 WP_003230039.1 rRNA maturation RNase YbeY -
  JNE32_RS14075 (JNE32_14010) pgpH 2656347..2658482 (-) 2136 WP_010886570.1 cyclic-di-AMP phosphodiesterase PgpH -

Sequence

Protein


Download         Length: 255 a.a.        Molecular weight: 29328.02 Da        Isoelectric Point: 8.3080

>NTDB_id=531742 JNE32_RS14045 WP_003230055.1 2653011..2653778(-) (recO) [Bacillus subtilis strain pb2441]
MLTKCEGIVLRTNDYGETNKIVTLLTREHGKIGVMARGAKKPNSRLSAVSQPFLYGSFLMQKTSGLGTLQQGEMILSMRG
IREDLFLTAYAAYVAELVDRGTEEKKPNPYLFEFILESLKQLNEGTDPDVITFIVQMKMLGVMGLYPELNHCVHCKSQDG
TFHFSVRDNGFICHRCFEKDPYRIPIKPQTARLLRLFYYFDLSRLGNVSLKEETKAELKQVIDLYYEEYSGLYLKSKRFL
DQMESMKHLMGENKS

Nucleotide


Download         Length: 768 bp        

>NTDB_id=531742 JNE32_RS14045 WP_003230055.1 2653011..2653778(-) (recO) [Bacillus subtilis strain pb2441]
ATGCTGACAAAATGTGAAGGGATCGTTCTTCGCACAAATGATTACGGAGAGACGAATAAAATCGTAACATTACTGACAAG
AGAACACGGAAAAATAGGTGTTATGGCACGAGGCGCCAAAAAACCGAACAGCCGTTTATCAGCAGTCAGCCAGCCCTTTT
TGTATGGTTCTTTCTTAATGCAAAAAACATCGGGCCTCGGAACGCTTCAGCAGGGGGAAATGATCCTCAGCATGAGAGGC
ATCAGGGAAGACTTGTTTTTAACAGCATATGCTGCCTATGTTGCTGAGTTAGTCGACAGAGGCACAGAAGAAAAAAAGCC
AAATCCTTATTTATTTGAATTCATATTAGAATCATTAAAACAGTTGAATGAAGGAACAGATCCGGATGTTATCACCTTCA
TTGTTCAAATGAAGATGCTCGGTGTCATGGGCCTGTATCCTGAGCTGAATCATTGTGTACATTGTAAAAGCCAAGACGGA
ACCTTTCATTTCTCTGTTCGTGATAATGGTTTTATATGCCACCGCTGTTTTGAAAAAGATCCTTACAGGATACCGATTAA
ACCGCAAACGGCAAGGCTGTTAAGGCTCTTTTACTATTTTGATCTGTCGAGACTCGGCAATGTGTCCTTAAAAGAGGAAA
CAAAAGCTGAATTAAAGCAAGTGATTGATTTATATTACGAAGAATATTCGGGATTGTATTTAAAATCAAAGCGCTTTTTA
GATCAAATGGAAAGCATGAAACATCTTATGGGTGAAAACAAAAGTTGA

Domains

Predicted by InterproScan.

Recombination protein O C terminal (RecO_C)

(82-237)

Recombination protein O N terminal (RecO_N)

(1-75)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recO Bacillus subtilis subsp. subtilis str. 168

100

100

1