Detailed information NTG0531701

Bioinformatically predicted

Overview

• Name: priA
• Type: Machinery gene
• Locus tag: K9N54_RS01265
• Genome accession: NZ_CP083361
• Coordinates: 266603..268807 (-)
• Length: 734 a.a.
• NCBI ID: WP_029824531.1
• Uniprot ID: -
• Organism: Vibrio parahaemolyticus strain 20-082E4
• Function: DNA puliing through the inner membrane (predicted from homology) DNA binding and uptake

Genomic Context

Location: 261603..273807

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
K9N54_RS01240 (K9N54_01245)	-	261822..262046 (+)	225	WP_005489715.1	hypothetical protein	-
K9N54_RS01245 (K9N54_01250)	hslU	262470..263801 (-)	1332	WP_005489452.1	HslU--HslV peptidase ATPase subunit	-
K9N54_RS01250 (K9N54_01255)	hslV	263825..264376 (-)	552	WP_005489705.1	ATP-dependent protease subunit HslV	-
K9N54_RS01255 (K9N54_01260)	ftsN	264541..265086 (-)	546	WP_005481405.1	cell division protein FtsN	-
K9N54_RS01260 (K9N54_01265)	cytR	265263..266270 (-)	1008	WP_005481416.1	DNA-binding transcriptional regulator CytR	Regulator
K9N54_RS01265 (K9N54_01270)	priA	266603..268807 (-)	2205	WP_029824531.1	primosomal protein N'	Machinery gene
K9N54_RS01270 (K9N54_01275)	rpmE	269102..269323 (+)	222	WP_005457203.1	50S ribosomal protein L31	-
K9N54_RS01275 (K9N54_01280)	rpsJ	269781..270092 (+)	312	WP_004410492.1	30S ribosomal protein S10	-
K9N54_RS01280 (K9N54_01285)	rplC	270107..270736 (+)	630	WP_005456132.1	50S ribosomal protein L3	-
K9N54_RS01285 (K9N54_01290)	rplD	270754..271356 (+)	603	WP_005379556.1	50S ribosomal protein L4	-
K9N54_RS01290 (K9N54_01295)	rplW	271353..271655 (+)	303	WP_004398471.1	50S ribosomal protein L23	-
K9N54_RS01295 (K9N54_01300)	rplB	271671..272495 (+)	825	WP_005489461.1	50S ribosomal protein L2	-
K9N54_RS01300 (K9N54_01305)	rpsS	272517..272795 (+)	279	WP_004394525.1	30S ribosomal protein S19	-
K9N54_RS01305 (K9N54_01310)	rplV	272806..273138 (+)	333	WP_005383164.1	50S ribosomal protein L22	-

Sequence

Protein

• Length: 734 a.a.
• Molecular weight: 82241.85 Da
• Isoelectric Point: 8.6772

>NTDB_id=531701 K9N54_RS01265 WP_029824531.1 266603..268807(-) (priA) [Vibrio parahaemolyticus strain 20-082E4]
MRPSIARVALPVPLDKQFDYAIPGHLFPIIGGRVSVPFGRQTLVGIVTAMVNHSDFPKDQLKPIKAVLDSQPIWPEKLYS
LLTWCSQFYQYPLGDTLHNAMPAALRKGKPADFATLQEWQITESGKDKLMQGLDRRAVKQQKVLQMLVNGALPHQEFVDQ
EIASTVLKSLEEKGWIERIEKKPVITQWGQHVECDVEKPKLNHEQALAIASVNSQTGFACYLLEGVTGSGKTEVYLNLIK
PVLEKGKQALVLVPEIGLTPQTINRFKRRFNVPVDVIHSGLNETERLNAWLSARDKAAGIIIGTRSALLAPFADLGIIIV
DEEHDTSYKQQDSLRYHARDVAVMRAHKEQIPIVLGSATPALETLHNALSGKYQHLTLTQRAGSAVPTTNKVLDVKGQYL
ESGLSAPLIAEMRKHLKAGNQVMLFLNRRGFSPALMCHECGWIAECKRCDAYYTFHQYSNEIRCHHCGSQHPVIHQCQGC
GSTQLVTVGVGTEQLEQQLAQLFPEYKAIRIDRDSTRRKGSLEDALDSIRKGEYQILIGTQMLAKGHHFPNVTLVALLDV
DGSLYSSDFRASERLAQLFIQVAGRAGRASKPGEVVLQTHHPEHSLLQALLEKDYRHFAMTALEERKLAQLPPYSFLTLF
KAEANQSEIVEDFLRQVRFTLESHPLFDGTCMVLGPTPSPLAKRAGKYRWQLLLQTQHRSLMQKLLTSAKPAIELLPNAK
KVRWNLDIEPQDLS

Nucleotide

• Length: 2205 bp

>NTDB_id=531701 K9N54_RS01265 WP_029824531.1 266603..268807(-) (priA) [Vibrio parahaemolyticus strain 20-082E4]
ATGCGTCCATCCATTGCTAGAGTGGCACTGCCCGTACCTCTCGACAAACAATTTGACTATGCCATCCCAGGACACCTGTT
TCCTATCATTGGCGGGCGCGTATCCGTACCTTTTGGACGTCAAACGTTAGTTGGCATCGTCACCGCGATGGTTAATCACT
CGGACTTTCCCAAAGATCAACTCAAGCCAATCAAAGCGGTTCTTGATTCTCAGCCGATATGGCCTGAAAAGCTTTATTCG
TTGCTGACTTGGTGCAGCCAGTTCTACCAATACCCACTTGGAGATACGCTACACAATGCGATGCCAGCAGCTCTGCGTAA
AGGAAAGCCTGCAGATTTTGCGACGCTACAAGAGTGGCAAATTACCGAATCCGGTAAAGACAAGCTGATGCAAGGGCTTG
ATCGTCGAGCGGTCAAACAACAGAAGGTCTTGCAAATGTTGGTTAACGGCGCTTTGCCTCACCAGGAATTTGTCGACCAA
GAGATAGCCTCAACAGTACTAAAGTCTTTAGAAGAGAAAGGCTGGATTGAACGTATCGAGAAGAAGCCTGTAATCACCCA
GTGGGGACAGCACGTTGAATGCGACGTCGAGAAGCCAAAGCTCAACCACGAACAAGCGCTGGCTATTGCGAGTGTAAATA
GCCAAACAGGTTTTGCTTGCTATTTATTAGAGGGCGTCACGGGCTCAGGTAAGACAGAAGTGTACCTTAACCTGATCAAG
CCGGTCTTAGAGAAAGGTAAACAAGCTTTGGTTTTAGTACCAGAGATTGGCTTAACACCGCAAACCATCAACCGCTTTAA
ACGCCGTTTTAATGTGCCAGTTGACGTTATCCACTCCGGATTAAACGAGACGGAGCGTCTGAATGCGTGGCTTTCTGCAC
GCGACAAAGCGGCCGGAATCATTATCGGCACACGCTCTGCCCTACTCGCGCCTTTTGCTGATCTCGGGATTATCATTGTG
GACGAGGAACATGACACCTCTTACAAACAGCAAGATAGCTTGCGTTATCATGCACGCGATGTTGCTGTAATGCGCGCTCA
TAAAGAACAAATTCCGATTGTTCTGGGCTCAGCCACACCTGCACTGGAGACATTGCACAATGCCTTGTCAGGCAAGTATC
AGCATTTAACCCTGACCCAACGCGCAGGCTCTGCGGTTCCTACTACTAATAAAGTTCTCGACGTCAAAGGTCAGTATTTA
GAAAGTGGTTTATCTGCCCCATTGATTGCGGAAATGCGTAAACACCTTAAAGCAGGCAATCAGGTGATGCTGTTTCTTAA
CCGTCGTGGTTTCTCCCCTGCTCTCATGTGTCATGAATGTGGTTGGATTGCCGAATGCAAACGTTGTGATGCTTATTACA
CCTTCCACCAATACAGCAATGAGATTCGCTGCCACCATTGTGGCTCACAGCACCCAGTGATTCATCAGTGTCAGGGCTGT
GGTTCAACTCAACTGGTTACCGTTGGTGTCGGTACCGAACAGTTAGAACAACAATTGGCACAATTGTTTCCCGAATACAA
AGCTATCCGTATCGACAGGGATAGTACGCGCCGCAAAGGTAGCTTAGAAGACGCGTTGGACTCCATCCGCAAGGGTGAAT
ATCAAATTTTAATTGGCACTCAAATGTTAGCTAAGGGACACCATTTCCCCAATGTAACGCTCGTTGCCTTGCTAGATGTT
GATGGCTCTCTATATAGTAGCGACTTCCGCGCCTCCGAAAGATTAGCGCAGTTATTCATTCAAGTCGCAGGGCGAGCCGG
ACGTGCAAGTAAGCCGGGGGAAGTGGTTTTGCAGACTCACCATCCTGAGCACAGTTTGTTGCAAGCCTTATTGGAAAAGG
ACTACCGACACTTCGCTATGACGGCATTAGAGGAACGTAAACTGGCGCAGTTGCCACCTTACAGTTTCCTGACTCTATTT
AAAGCCGAAGCGAATCAGAGCGAGATCGTAGAAGATTTTCTGCGCCAAGTTCGCTTTACCTTAGAATCGCACCCTTTGTT
TGACGGCACCTGCATGGTGCTTGGTCCAACACCATCGCCTCTCGCCAAACGCGCAGGCAAATACCGTTGGCAGCTGTTAC
TCCAAACCCAGCACCGTTCATTAATGCAAAAGTTATTAACCAGTGCAAAACCTGCTATTGAGTTGTTGCCTAATGCCAAA
AAAGTTCGCTGGAATTTAGACATAGAACCACAAGATCTCAGCTAG

Domains

Predicted by InterProScan.

• 3DNA-binding domain (3BD) (PriA_3primeBD) (7-102)

• PriA DNA helicase Cys-rich region (CRR) domain (Zn_ribbon_PriA) (446-472)

• Primosomal protein N C-terminal domain (PriA_C) (633-730)

• Helicase conserved C-terminal domain (Helicase_C) (500-589)

• DEAD/DEAH box helicase (DEAD) (220-365)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
priA	Acinetobacter baumannii D1279779	43.649	100	0.44
priA	Bacillus subtilis subsp. subtilis str. 168	34.005	100	0.368