Detailed information NTG0531688

Bioinformatically predicted

Overview

• Name: amiE
• Type: Regulator
• Locus tag: K6U01_RS21145
• Genome accession: NZ_CP083274
• Coordinates: 4373418..4374401 (-)
• Length: 327 a.a.
• NCBI ID: WP_001196486.1
• Uniprot ID: A7ZT85
• Organism: Escherichia coli strain Rosetta2-DE3
• Function: internalize XIP (predicted from homology) Competence regulation

Genomic Context

Location: 4368418..4379401

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
K6U01_RS21115 (K6U01_21055)	pnuC	4368425..4369144 (-)	720	WP_000345410.1	nicotinamide riboside transporter PnuC	-
K6U01_RS21120 (K6U01_21060)	nadA	4369182..4370225 (-)	1044	WP_000115290.1	quinolinate synthase NadA	-
K6U01_RS21135 (K6U01_21075)	yhjV	4371116..4372387 (+)	1272	WP_001295225.1	aromatic amino acid transport family protein	-
K6U01_RS21140 (K6U01_21080)	dppF	4372417..4373421 (-)	1005	WP_000107012.1	dipeptide ABC transporter ATP-binding subunit DppF	-
K6U01_RS21145 (K6U01_21085)	amiE	4373418..4374401 (-)	984	WP_001196486.1	dipeptide ABC transporter ATP-binding protein	Regulator
K6U01_RS21150 (K6U01_21090)	dppC	4374412..4375314 (-)	903	WP_000084677.1	dipeptide ABC transporter permease DppC	-
K6U01_RS21155 (K6U01_21095)	dppB	4375324..4376343 (-)	1020	WP_000938855.1	dipeptide ABC transporter permease DppB	-
K6U01_RS21160 (K6U01_21100)	dppA	4376651..4378258 (-)	1608	WP_001222883.1	dipeptide ABC transporter substrate-binding protein DppA	-
K6U01_RS21165 (K6U01_21105)	rbsD	4379017..4379253 (+)	237	Protein_4179	D-ribose pyranase	-

Sequence

Protein

• Length: 327 a.a.
• Molecular weight: 35844.36 Da
• Isoelectric Point: 6.5814

>NTDB_id=531688 K6U01_RS21145 WP_001196486.1 4373418..4374401(-) (amiE) [Escherichia coli strain Rosetta2-DE3]
MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGSGKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISE
KERRNLVGAEVAMIFQDPMTSLNPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVAEAAHKIIVMYAGQVVETGDAHA
IFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPGKYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLD
DAGRPTL

Nucleotide

• Length: 984 bp

>NTDB_id=531688 K6U01_RS21145 WP_001196486.1 4373418..4374401(-) (amiE) [Escherichia coli strain Rosetta2-DE3]
ATGGCGTTATTAAATGTAGATAAATTATCGGTGCATTTCGGCGACGAAAGCGCGCCGTTCCGCGCCGTAGACCGCATCAG
CTACAGCGTAAAACAGGGCGAAGTGGTCGGGATTGTGGGTGAGTCCGGCTCCGGTAAGTCGGTCAGTTCACTGGCGATTA
TGGGGCTGATTGATTATCCGGGCCGCGTAATGGCAGAAAAACTGGAGTTTAACGGCCAGGATTTGCAGCGTATCTCAGAA
AAAGAGCGCCGCAACCTGGTGGGTGCCGAAGTGGCGATGATCTTCCAGGACCCGATGACCAGCCTTAACCCGTGCTACAC
CGTGGGTTTCCAGATTATGGAAGCGATTAAGGTGCATCAGGGCGGCAACAAAAGTACCCGCCGTCAGCGAGCGATTGACC
TGCTGAATCAGGTCGGTATTCCCGATCCGGCATCGCGTCTGGATGTTTACCCGCATCAGCTTTCCGGCGGCATGAGCCAG
CGCGTGATGATCGCCATGGCGATTGCCTGTCGGCCAAAACTGCTGATTGCCGATGAACCGACCACCGCGCTGGACGTGAC
CATTCAGGCGCAAATCATCGAACTACTGCTGGAGCTACAGCAGAAAGAGAACATGGCGCTGGTGTTAATTACCCATGACC
TGGCGCTGGTGGCGGAAGCGGCACATAAAATCATCGTGATGTATGCAGGCCAGGTGGTGGAAACCGGTGATGCGCACGCC
ATCTTCCATGCGCCGCGTCACCCGTATACTCAGGCATTGCTGCGTGCGCTGCCAGAATTTGCTCAGGACAAAGAACGTCT
GGCGTCGTTGCCAGGTGTCGTTCCCGGCAAGTACGACCGCCCGAACGGCTGCCTGCTTAACCCGCGCTGCCCCTATGCCA
CTGACAGATGTCGCGCTGAAGAACCGGCGCTGAATATGCTCGCTGACGGGCGTCAGTCCAAATGCCATTACCCACTTGAT
GATGCCGGGAGGCCGACACTATGA

Domains

Predicted by InterProScan.

• Oligopeptide/dipeptide transporter, C-terminal region (oligo_HPY) (233-297)

• ABC transporter (ABC_tran) (24-181)

3D structure

• AlphaFold DB: A7ZT85

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
amiE	Streptococcus salivarius strain HSISS4	43.302	98.165	0.425
amiE	Streptococcus thermophilus LMG 18311	42.991	98.165	0.422
amiE	Streptococcus thermophilus LMD-9	42.991	98.165	0.422
oppD	Streptococcus mutans UA159	41.379	97.554	0.404