Detailed information    

insolico Bioinformatically predicted

Overview

Name   sbcB   Type   Machinery gene
Locus tag   K6U01_RS14140 Genome accession   NZ_CP083274
Coordinates   2943333..2944760 (+) Length   475 a.a.
NCBI ID   WP_000980563.1    Uniprot ID   -
Organism   Escherichia coli strain Rosetta2-DE3     
Function   promote homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2938333..2949760
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K6U01_RS14105 (K6U01_14065) - 2938745..2938837 (+) 93 Protein_2787 DUF957 domain-containing protein -
  K6U01_RS14110 (K6U01_14070) - 2939126..2939263 (-) 138 Protein_2788 transposase domain-containing protein -
  K6U01_RS14115 (K6U01_14075) - 2939326..2939508 (+) 183 WP_001016348.1 EutP/PduV family microcompartment system protein -
  K6U01_RS14120 (K6U01_14080) tmaR 2939609..2939938 (-) 330 WP_000450409.1 DUF496 family protein -
  K6U01_RS14125 (K6U01_14085) yeeA 2940110..2941168 (-) 1059 WP_001200905.1 FUSC family protein -
  K6U01_RS14130 (K6U01_14090) sbmC 2941366..2941839 (-) 474 WP_001105393.1 DNA gyrase inhibitor SbmC -
  K6U01_RS14135 (K6U01_14095) dacD 2941958..2943124 (-) 1167 WP_001320295.1 serine-type D-Ala-D-Ala carboxypeptidase DacD -
  K6U01_RS14140 (K6U01_14100) sbcB 2943333..2944760 (+) 1428 WP_000980563.1 exodeoxyribonuclease I Machinery gene
  K6U01_RS14145 (K6U01_14105) tsuB 2944803..2945030 (-) 228 WP_000985273.1 thiosulfate utilization sulfurtransferase TsuB/YeeD -
  K6U01_RS14150 (K6U01_14110) tsuA 2945044..2946102 (-) 1059 WP_000492348.1 thiosulfate utilization transporter TsuA/YeeE -
  K6U01_RS14155 (K6U01_14115) plaP 2946281..2947639 (-) 1359 WP_000019197.1 putrescine/proton symporter PlaP -
  K6U01_RS14160 (K6U01_14120) yoeI 2947629..2947691 (-) 63 WP_010723108.1 membrane protein YoeI -
  K6U01_RS14165 (K6U01_14125) yeeY 2947906..2948835 (-) 930 WP_000803363.1 LysR substrate-binding domain-containing protein -
  K6U01_RS14170 (K6U01_14130) yeeZ 2948881..2949705 (-) 825 WP_000754737.1 SDR family oxidoreductase -

Sequence

Protein


Download         Length: 475 a.a.        Molecular weight: 54484.77 Da        Isoelectric Point: 5.6054

>NTDB_id=531665 K6U01_RS14140 WP_000980563.1 2943333..2944760(+) (sbcB) [Escherichia coli strain Rosetta2-DE3]
MMNDGKQQSTFLFHDYETFGTHPALDRPAQFAAIRTDNEFNVIGEPEVFYCKPADDYLPQPGAVLITGITPQEARAKGEN
EAAFAARIHSLFTVPKTCILGYNNVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDG
LPSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLMALIDVPQMKPLVHVSGMFGAWR
GNTSWVAPLAWHPENRNAVIMVDLAGDISPLLELDSDTLRERLYTAKTDLGDNAAVPVKLVHINKCPVLAQANTLRPEDA
DRLGINRQHCLDNLKILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLPALDITFVD
KRIEKLLFNYRARNFPGTLDYAEQQRWLEHRRQVFTPEFLQGYADELQMLAQQYADDKEKVALLKALWQYAQEIV

Nucleotide


Download         Length: 1428 bp        

>NTDB_id=531665 K6U01_RS14140 WP_000980563.1 2943333..2944760(+) (sbcB) [Escherichia coli strain Rosetta2-DE3]
ATGATGAATGACGGTAAGCAACAATCTACCTTTTTGTTTCACGATTACGAAACCTTTGGAACGCACCCCGCGTTAGATCG
CCCTGCACAGTTCGCAGCCATTCGCACTGATAACGAATTCAATGTCATCGGCGAACCCGAAGTCTTTTACTGCAAGCCCG
CGGATGACTATTTACCCCAGCCTGGAGCAGTATTAATTACCGGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAAC
GAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTT
CGACGATGAAGTCACACGCAACGTTTTTTATCGTAATTTCTACGATCCTTACGCCTGGAGCTGGCAGCATGATAACTCGC
GCTGGGATTTACTGGATGTTATGCGTGCCTGTTATGCCCTGCGCCCGGAAGGAATAAACTGGCCTGAAAATGATGACGGT
CTACCGAGCTTTCGCCTTGAGCATTTAACCAAAGCGAATGGTATTGAACATAGCAACGCCCACGATGCGATGGCTGATGT
GTACGCCACTATTGCGATGGCGAAACTGGTAAAAACGCGTCAGCCACGCCTGTTTGATTATCTCTTTACCCATCGTAATA
AACACAAACTGATGGCGTTGATTGATGTTCCGCAGATGAAACCCCTGGTTCACGTTTCCGGAATGTTTGGGGCATGGCGT
GGCAATACCAGCTGGGTGGCACCGCTGGCGTGGCATCCTGAAAATCGCAATGCCGTAATTATGGTGGATTTGGCAGGAGA
CATTTCGCCATTACTGGAACTGGATAGCGACACATTGCGCGAGCGTTTATATACCGCAAAAACCGATCTTGGCGATAACG
CCGCCGTTCCGGTTAAGCTGGTGCATATCAATAAATGTCCGGTACTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCC
GACCGACTGGGAATTAATCGTCAGCATTGCCTCGATAACCTAAAAATTCTGCGTGAAAATCCGCAAGTGCGTGAAAAAGT
GGTGGCGATATTCGCGGAAGCCGAACCGTTTACGCCTTCAGATAACGTGGATGCACAGCTTTATAATGGCTTTTTCAGTG
ACGCCGATCGTGCAGCAATGAAAATTGTGCTGGAAACCGAGCCGCGTAATTTACCGGCACTGGATATCACTTTTGTTGAT
AAACGAATTGAAAAACTGTTGTTCAATTATCGAGCGCGCAACTTCCCGGGGACGCTGGATTATGCCGAGCAGCAACGCTG
GCTTGAGCACCGCCGCCAGGTCTTCACGCCAGAGTTTTTGCAGGGCTACGCTGATGAATTGCAGATGCTGGCACAACAGT
ATGCCGATGACAAAGAGAAAGTGGCGCTGTTAAAAGCGCTTTGGCAGTACGCGCAAGAGATTGTCTAA

Domains

Predicted by InterProScan.

Exonuclease (RNase_T)

(13-188)

Exonuclease C-terminal (ExoI_SH3)

(212-472)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  sbcB Vibrio cholerae O1 biovar El Tor strain E7946

58.723

98.947

0.581