Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   K9C16_RS16680 Genome accession   NZ_CP082952
Coordinates   3464625..3465425 (-) Length   266 a.a.
NCBI ID   WP_004738086.1    Uniprot ID   -
Organism   Acinetobacter baumannii strain Cl300     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3459625..3470425
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K9C16_RS16660 (K9C16_16590) - 3460690..3461388 (-) 699 WP_000058788.1 YfgM family protein -
  K9C16_RS16665 (K9C16_16595) hisS 3461417..3462709 (-) 1293 WP_000095254.1 histidine--tRNA ligase -
  K9C16_RS16670 (K9C16_16600) ispG 3462706..3463821 (-) 1116 WP_000572095.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  K9C16_RS16675 (K9C16_16605) - 3463843..3464634 (-) 792 WP_000405163.1 helix-turn-helix domain-containing protein -
  K9C16_RS16680 (K9C16_16610) pilF 3464625..3465425 (-) 801 WP_004738086.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  K9C16_RS16685 (K9C16_16615) rlmN 3465441..3466673 (-) 1233 WP_000093084.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  K9C16_RS16690 (K9C16_16620) ndk 3466796..3467227 (-) 432 WP_000963851.1 nucleoside-diphosphate kinase -
  K9C16_RS16695 (K9C16_16625) iscX 3467341..3467541 (-) 201 WP_000524329.1 Fe-S cluster assembly protein IscX -
  K9C16_RS16700 (K9C16_16630) - 3467641..3468192 (-) 552 WP_000669565.1 phosphatase PAP2 family protein -
  K9C16_RS16705 (K9C16_16635) - 3468217..3469515 (-) 1299 WP_086221435.1 glycosyltransferase family 4 protein -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 30094.44 Da        Isoelectric Point: 9.2307

>NTDB_id=531036 K9C16_RS16680 WP_004738086.1 3464625..3465425(-) (pilF) [Acinetobacter baumannii strain Cl300]
MSTPKLKIMLCMGVAVALLASGCQTSQTVKKDPEKAVKVRTQLAAEYIRSGDLDSAKRSLDQALSVDSRDATANMMMGIL
LQQEGSKPNLEKAEHYFKRAISSEPDNAQARNNYGTYLYQMERYNDAIEQFRIAGATLGYDQRYQALENLGRIYLKLGDI
ASAEKTFKQALLANRDSYISMLELAEIFYLQQQIPAATQMYEQYVRTVGQKNQGARALWIGLRVARANADKMGMQVLVNQ
LRALFPESPEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 801 bp        

>NTDB_id=531036 K9C16_RS16680 WP_004738086.1 3464625..3465425(-) (pilF) [Acinetobacter baumannii strain Cl300]
TTGAGTACTCCGAAGTTGAAGATCATGTTATGTATGGGGGTTGCTGTTGCACTTTTGGCAAGTGGTTGTCAGACTTCGCA
AACGGTTAAAAAGGACCCGGAAAAAGCAGTAAAAGTGCGTACGCAGCTTGCTGCCGAATACATTCGTTCTGGTGACTTGG
ATTCTGCAAAGCGTTCTCTTGACCAAGCGTTAAGTGTCGATAGTCGTGATGCAACGGCAAATATGATGATGGGTATTTTA
TTGCAACAAGAAGGCAGTAAACCCAATTTAGAAAAAGCGGAACATTACTTTAAGCGTGCAATTTCGTCTGAACCAGACAA
TGCTCAGGCACGTAATAATTATGGTACGTATTTGTACCAAATGGAGCGTTATAATGATGCTATTGAGCAATTTCGTATTG
CTGGTGCAACATTAGGCTATGATCAACGTTACCAGGCTTTAGAAAATCTGGGGCGAATTTATTTAAAACTAGGCGATATT
GCGAGTGCTGAAAAAACATTCAAACAAGCACTTCTTGCTAACCGCGACTCTTACATTTCAATGTTAGAGTTAGCAGAAAT
TTTTTATTTGCAGCAGCAGATTCCGGCTGCTACACAAATGTATGAACAATATGTTCGTACAGTGGGGCAGAAAAATCAGG
GTGCACGCGCACTTTGGATTGGTTTAAGAGTGGCTCGAGCCAATGCGGATAAAATGGGAATGCAAGTTTTAGTAAACCAG
TTACGTGCTTTATTCCCGGAAAGTCCAGAATATCAACGTTATTTGCAATTACAGTATAGTACTGAGGCCGTATGGAAATA
A

Domains

Predicted by InterProScan.

Tetratricopeptide repeat (TPR_8)

(39-68)

Tetratricopeptide repeat (TPR_16)

(89-134)

Tetratricopeptide repeat (TPR_16)

(149-206)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

100

100

1
  pilF Acinetobacter baylyi ADP1

74.231

97.744

0.726