Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   JMB28_RS12130 Genome accession   NZ_CP068712
Coordinates   2533115..2534485 (-) Length   456 a.a.
NCBI ID   WP_069792830.1    Uniprot ID   -
Organism   Staphylococcus shinii strain 14AME19     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2528115..2539485
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JMB28_RS12115 (JMB28_12115) cysE 2529093..2529734 (-) 642 WP_039068620.1 serine O-acetyltransferase -
  JMB28_RS12120 (JMB28_12120) gltX 2530087..2531541 (-) 1455 WP_069792829.1 glutamate--tRNA ligase -
  JMB28_RS12125 (JMB28_12125) - 2531976..2533034 (-) 1059 WP_039068601.1 PIN/TRAM domain-containing protein -
  JMB28_RS12130 (JMB28_12130) radA 2533115..2534485 (-) 1371 WP_069792830.1 DNA repair protein RadA Machinery gene
  JMB28_RS12135 (JMB28_12135) - 2534829..2537291 (-) 2463 WP_039068604.1 ATP-dependent Clp protease ATP-binding subunit -
  JMB28_RS12140 (JMB28_12140) - 2537305..2538315 (-) 1011 WP_069792831.1 protein arginine kinase -
  JMB28_RS12145 (JMB28_12145) - 2538308..2538859 (-) 552 WP_042363311.1 UvrB/UvrC motif-containing protein -
  JMB28_RS12150 (JMB28_12150) - 2538876..2539337 (-) 462 WP_017723054.1 CtsR family transcriptional regulator -

Sequence

Protein


Download         Length: 456 a.a.        Molecular weight: 50415.95 Da        Isoelectric Point: 7.4847

>NTDB_id=530617 JMB28_RS12130 WP_069792830.1 2533115..2534485(-) (radA) [Staphylococcus shinii strain 14AME19]
MAKKKVIFECMACGYQSPKWMGKCPNCGGWNQMEEIVEQKAASPKHGVRSRDNISKVQKLNNIKHETTPRVKTNSEEFNR
VLGGGIVNGSLVLIGGDPGIGKSTLLLQICAALSQSKNVLYITGEESLNQTKLRAERLDEDSSELNVFAETDLEVIHETV
KQLKPDLLVVDSIQTIFHPEISSAPGSVSQVRESTQSLMNIAKQLNIATFIVGHVTKEGQIAGPRLLEHMVDTVLYFEGD
EHHAYRILRAVKNRFGSTNEMGIFEMKQTGLKGVKNPSEMFLEERSTNVPGSTIVSTMEGTRPLLIEVQALVTPTTFNNP
RRMATGIDHNRLSLLMAVLEKKENYLLQQQDAYIKVAGGVRLTEPAVDLGIVVATASSFKDLTVDGLDCFIGEVGLTGEV
RRVSRIEQRVQEAEKLGFKRVIIPESNIGGWQFPNDIKVIGVKNVHEALKYALTKH

Nucleotide


Download         Length: 1371 bp        

>NTDB_id=530617 JMB28_RS12130 WP_069792830.1 2533115..2534485(-) (radA) [Staphylococcus shinii strain 14AME19]
TTGGCTAAAAAGAAAGTAATTTTTGAATGTATGGCCTGTGGTTACCAGTCTCCAAAGTGGATGGGGAAATGCCCGAATTG
TGGTGGTTGGAACCAAATGGAAGAAATAGTCGAACAAAAGGCCGCGAGTCCAAAACATGGCGTACGCAGTCGAGACAATA
TTTCTAAAGTACAAAAATTAAATAATATTAAACATGAAACAACGCCACGAGTAAAAACAAATTCTGAAGAATTTAATAGA
GTGCTTGGTGGCGGTATAGTAAATGGCTCACTTGTATTGATAGGTGGCGATCCGGGTATCGGTAAGTCTACATTACTTTT
ACAAATTTGTGCAGCATTATCACAATCTAAAAATGTACTGTATATTACTGGTGAAGAATCATTAAATCAAACGAAATTAA
GAGCGGAAAGATTAGACGAAGATTCAAGCGAATTAAATGTGTTTGCTGAAACAGATTTAGAAGTAATACATGAAACAGTT
AAACAACTTAAACCAGATTTACTTGTAGTAGACTCAATTCAAACTATTTTTCATCCAGAAATTAGTTCTGCGCCAGGTTC
TGTGTCCCAAGTTAGAGAAAGTACGCAAAGTTTGATGAATATAGCGAAACAATTAAATATAGCTACATTTATTGTGGGAC
ATGTTACGAAAGAAGGCCAAATAGCTGGCCCGCGTTTACTGGAACATATGGTAGATACAGTCCTATACTTTGAAGGTGAT
GAGCATCACGCGTATCGTATTTTAAGAGCAGTTAAAAACCGTTTTGGTTCTACAAACGAGATGGGCATCTTCGAAATGAA
ACAAACTGGGCTAAAAGGCGTGAAAAACCCCTCAGAAATGTTCCTTGAAGAACGATCAACGAATGTGCCTGGTTCGACCA
TTGTTTCTACGATGGAAGGTACAAGACCATTACTTATAGAAGTACAAGCATTAGTGACACCTACTACATTTAATAATCCA
AGACGTATGGCGACGGGCATCGATCATAATAGATTGAGCTTACTGATGGCAGTATTGGAAAAGAAAGAGAATTATTTACT
ACAACAACAAGATGCTTATATTAAAGTGGCAGGAGGCGTACGTTTAACAGAACCAGCTGTAGATTTAGGTATTGTTGTTG
CGACAGCATCAAGTTTCAAAGATTTAACAGTCGACGGTTTAGACTGTTTTATTGGAGAAGTAGGGCTTACAGGAGAAGTT
AGACGTGTTTCACGTATAGAACAACGTGTACAAGAAGCAGAAAAATTAGGGTTTAAGCGTGTGATTATTCCGGAATCAAA
TATAGGTGGATGGCAATTTCCAAATGACATCAAGGTCATAGGTGTTAAAAATGTCCATGAAGCATTAAAATATGCACTAA
CCAAACATTAA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(67-219)

Subunit ChlI of Mg-chelatase (ChlI)

(356-427)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

65.351

100

0.654
  radA Streptococcus pneumoniae Rx1

60

98.684

0.592
  radA Streptococcus pneumoniae D39

60

98.684

0.592
  radA Streptococcus pneumoniae R6

60

98.684

0.592
  radA Streptococcus pneumoniae TIGR4

60

98.684

0.592
  radA Streptococcus mitis NCTC 12261

60

98.684

0.592
  radA Streptococcus mitis SK321

60

98.684

0.592