Detailed information NTG0530487

Bioinformatically predicted

Overview

• Name: radA
• Type: Machinery gene
• Locus tag: LLUC073_RS11445
• Genome accession: NZ_CP068698
• Coordinates: 2222908..2224269 (-)
• Length: 453 a.a.
• NCBI ID: WP_011677138.1
• Uniprot ID: A0A165G707
• Organism: Lactococcus cremoris strain UC073
• Function: homologous recombination (predicted from homology) Homologous recombination

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
IS/Tn	2221917..2222807	2222908..2224269	flank	101

Gene organization within MGE regions

Location: 2221917..2224269

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LLUC073_RS11440 (LLUC073_11175)	-	2221917..2222807 (+)	891	WP_318299072.1	IS982-like element IS982B family transposase	-
LLUC073_RS11445 (LLUC073_11180)	radA	2222908..2224269 (-)	1362	WP_011677138.1	DNA repair protein RadA	Machinery gene

Sequence

Protein

• Length: 453 a.a.
• Molecular weight: 49897.33 Da
• Isoelectric Point: 5.2965

>NTDB_id=530487 LLUC073_RS11445 WP_011677138.1 2222908..2224269(-) (radA) [Lactococcus cremoris strain UC073]
MAKKKSSFVCQNCGYKSAKYLGRCPNCGEWSSFVEEVEVQEVKNQRVSMSGERSKPMKLDEVELFDTPRIETDLDEFNRV
LGGGVVPGSLVLIGGDPGIGKSTLLLQVSTQLASRGRVLYVSGEESAQQIKLRAERLGDIDRDFYLYAETNMQSIRAEVE
RLKPNFLIIDSIQTIMTPEIQSTQGSVSQVREVTGELMQIAKTNDIATFIVGHVTKEGQLAGPRMLEHMVDTVLYFEGER
NNTFRILRAVKNRFGSTNEIGIFEMQGHGLVEVTNPSEVFLEERLEGSTGSAIVCALEGTRPILVEIQALTTPTMFGNAK
RTTSGLDFNRVSLIMAVLEKRTGLLMQNQDAYLKSAGGVKLDEPAIDLAVAVAVASSYKELPTDARECFIGEIGLTGEIR
RVTRIEQRLNEAAKLGFKKVYAPKNSIVGIDIPEQIKVIGVTTLTECLKLVFG

Nucleotide

• Length: 1362 bp

>NTDB_id=530487 LLUC073_RS11445 WP_011677138.1 2222908..2224269(-) (radA) [Lactococcus cremoris strain UC073]
ATGGCGAAGAAAAAATCATCTTTTGTTTGTCAAAATTGTGGTTATAAATCTGCAAAGTATCTTGGGCGATGCCCAAATTG
TGGAGAGTGGTCCTCTTTTGTTGAAGAAGTTGAAGTACAGGAAGTCAAAAATCAGCGAGTGTCGATGTCTGGTGAACGTT
CAAAACCAATGAAATTAGATGAAGTTGAGCTTTTTGATACACCACGTATTGAAACAGATCTTGACGAATTTAATCGTGTT
TTAGGTGGTGGTGTTGTTCCTGGTTCACTCGTTTTGATTGGTGGAGACCCAGGAATAGGAAAATCTACGTTGCTTTTGCA
AGTATCGACTCAGCTTGCATCAAGAGGACGGGTTCTCTATGTGAGTGGTGAAGAATCTGCTCAACAAATTAAGCTTAGAG
CGGAACGTTTGGGCGATATTGATCGAGATTTTTATCTTTACGCCGAGACTAACATGCAGTCAATTCGTGCTGAAGTTGAA
CGTTTGAAGCCAAATTTTTTGATTATTGATTCGATCCAAACTATTATGACACCTGAAATTCAATCAACTCAAGGCTCTGT
GAGTCAAGTTCGAGAAGTGACAGGTGAACTCATGCAAATTGCCAAAACGAATGATATTGCTACTTTTATCGTTGGTCATG
TGACGAAAGAAGGTCAATTGGCAGGACCAAGAATGCTAGAGCACATGGTTGATACCGTTCTTTATTTTGAAGGAGAAAGA
AATAATACTTTTAGAATTCTTCGAGCAGTTAAAAACCGGTTTGGGTCAACCAATGAAATTGGTATATTCGAAATGCAAGG
GCATGGTTTAGTTGAAGTAACTAATCCTTCTGAGGTTTTCTTGGAAGAGCGATTAGAGGGTTCAACTGGTTCTGCTATTG
TTTGTGCACTCGAAGGGACACGTCCGATATTAGTTGAAATACAGGCTTTAACTACACCAACAATGTTTGGAAATGCTAAG
AGAACAACTTCTGGTTTAGATTTTAATAGAGTGAGCCTGATTATGGCAGTACTTGAAAAACGGACAGGATTATTGATGCA
AAATCAAGATGCTTACCTTAAATCAGCTGGGGGAGTAAAATTAGATGAGCCAGCCATTGACCTTGCGGTTGCTGTCGCTG
TAGCCTCAAGTTATAAAGAACTTCCGACGGATGCGCGTGAATGTTTTATTGGTGAAATTGGCTTAACAGGAGAGATTCGT
CGTGTGACGCGAATTGAACAAAGACTGAATGAAGCTGCTAAATTAGGATTCAAAAAAGTCTATGCTCCTAAAAATTCAAT
TGTGGGAATTGATATTCCTGAACAAATAAAAGTAATTGGGGTAACAACCCTAACTGAATGTCTAAAATTAGTATTTGGCT
AA

Domains

Predicted by InterproScan.

• AAA domain (AAA_25) (70-218)

• Rubredoxin metal binding domain (Zn_ribbon_LapB) (8-35)

• Subunit ChlI of Mg-chelatase (ChlI) (360-425)

3D structure

• AlphaFold DB: A0A165G707

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
radA	Streptococcus pneumoniae Rx1	81.015	100	0.81
radA	Streptococcus pneumoniae D39	81.015	100	0.81
radA	Streptococcus pneumoniae R6	81.015	100	0.81
radA	Streptococcus pneumoniae TIGR4	81.015	100	0.81
radA	Streptococcus mitis SK321	81.015	100	0.81
radA	Streptococcus mitis NCTC 12261	80.795	100	0.808
radA	Bacillus subtilis subsp. subtilis str. 168	61.707	100	0.623