Detailed information NTG0530382

Bioinformatically predicted

Overview

• Name: radA
• Type: Machinery gene
• Locus tag: JKV48_RS12810
• Genome accession: NZ_CP068682
• Coordinates: 2624468..2625832 (-)
• Length: 454 a.a.
• NCBI ID: WP_001085219.1
• Uniprot ID: W8U6A9
• Organism: Staphylococcus aureus strain NCCP11854
• Function: homologous recombination (predicted from homology) Homologous recombination

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
IS/Tn	2626396..2626881	2624468..2625832	flank	564

Gene organization within MGE regions

Location: 2624468..2626881

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
JKV48_RS12810 (JKV48_12755)	radA	2624468..2625832 (-)	1365	WP_001085219.1	DNA repair protein RadA	Machinery gene
JKV48_RS12815 (JKV48_12760)	tnpA	2626396..2626881 (-)	486	WP_000093393.1	IS200/IS605 family transposase	-

Sequence

Protein

• Length: 454 a.a.
• Molecular weight: 49835.35 Da
• Isoelectric Point: 7.0049

>NTDB_id=530382 JKV48_RS12810 WP_001085219.1 2624468..2625832(-) (radA) [Staphylococcus aureus strain NCCP11854]
MAKKKVIFECMACGYQSPKWMGKCPNCGAWNQMEEIVEKAANPKHGVKTKELAGKVQKLNSIKHETTPRVLTDSAEFNRV
LGGGIVSGSLVLIGGDPGIGKSTLLLQICASLSQKKKVLYITGEESLSQTKLRAERLDEDSSELQVLAETDLEVIYQTVK
EEQPDLLVVDSIQTIYHPEISSAPGSVSQVRESTQSLMNIAKQMNIATFIVGHVTKEGQIAGPRLLEHMVDTVLYFEGDE
HHAYRILRAVKNRFGSTNEMGIFEMKQSGLKGVNNPSEMFLEERSTNVPGSTIVATMEGTRPLLIEVQALVTPTTFNNPR
RMATGIDHNRLSLLMAVLEKKENYLLQQQDAYIKVAGGVKLTEPAVDLSVIVATASSFKDKAVDGLDCYIGEVGLTGEVR
RVSRIEQRVQEAAKLGFKRVIIPKNNIGGWTYPEGIQVIGVTTVHEALSFALHS

Nucleotide

• Length: 1365 bp

>NTDB_id=530382 JKV48_RS12810 WP_001085219.1 2624468..2625832(-) (radA) [Staphylococcus aureus strain NCCP11854]
TTGGCCAAGAAAAAAGTGATTTTTGAATGTATGGCTTGTGGTTATCAATCTCCTAAATGGATGGGGAAATGTCCTAATTG
TGGCGCTTGGAATCAAATGGAGGAAATTGTTGAAAAAGCAGCCAATCCTAAACATGGAGTGAAAACCAAGGAATTAGCAG
GTAAAGTACAAAAATTAAATAGTATTAAACATGAAACAACGCCGAGAGTGTTAACAGATTCAGCAGAATTCAACCGTGTA
TTAGGTGGAGGTATTGTGAGCGGATCGTTAGTACTTATTGGTGGGGATCCAGGTATTGGTAAGTCAACGTTACTTTTACA
AATTTGTGCATCGTTATCTCAAAAGAAAAAAGTACTATATATTACTGGAGAAGAATCGCTTAGTCAGACTAAATTACGTG
CAGAGCGATTAGATGAAGATTCAAGTGAATTGCAAGTATTAGCTGAAACAGATCTTGAAGTTATTTATCAAACAGTAAAA
GAAGAACAACCTGATTTATTAGTAGTGGATTCGATTCAAACAATATATCATCCTGAAATCAGCTCTGCGCCAGGTTCTGT
TTCACAAGTTCGTGAAAGTACACAAAGTTTAATGAATATTGCTAAACAAATGAACATTGCAACTTTTATAGTGGGTCATG
TAACGAAAGAAGGTCAAATTGCTGGCCCAAGATTGCTAGAACACATGGTTGATACTGTGCTTTATTTTGAAGGCGATGAA
CATCACGCATATCGAATTTTGCGAGCTGTTAAAAACCGTTTTGGTTCAACGAATGAAATGGGAATCTTCGAAATGAAGCA
AAGTGGATTAAAAGGTGTCAATAATCCATCTGAAATGTTTTTAGAAGAACGTTCAACAAATGTTCCAGGTTCAACAATTG
TTGCAACCATGGAGGGAACCAGACCACTTTTAATAGAAGTTCAAGCGCTGGTAACTCCAACGACTTTTAACAATCCGAGA
CGAATGGCAACAGGGATTGATCATAATCGATTAAGTTTGTTGATGGCTGTTTTGGAAAAGAAAGAAAATTATCTATTACA
ACAACAAGATGCTTATATCAAAGTAGCTGGCGGTGTAAAGTTAACGGAGCCAGCAGTTGATTTAAGTGTAATTGTAGCAA
CTGCGTCTAGCTTTAAAGATAAAGCTGTCGACGGATTAGATTGCTATATTGGAGAAGTTGGTTTAACGGGTGAGGTACGT
CGTGTATCTCGGATAGAACAACGCGTGCAAGAGGCTGCAAAACTAGGTTTCAAACGTGTAATTATTCCTAAAAATAATAT
AGGCGGATGGACATATCCTGAAGGTATACAAGTAATAGGTGTAACTACTGTACATGAAGCATTGTCATTTGCTCTTCATT
CATAA

Domains

Predicted by InterproScan.

• Rubredoxin metal binding domain (Zn_ribbon_LapB) (8-35)

• AAA domain (AAA_25) (77-218)

• Subunit ChlI of Mg-chelatase (ChlI) (353-426)

3D structure

• AlphaFold DB: W8U6A9

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
radA	Bacillus subtilis subsp. subtilis str. 168	66.667	100	0.674
radA	Streptococcus pneumoniae Rx1	62.054	98.678	0.612
radA	Streptococcus pneumoniae D39	62.054	98.678	0.612
radA	Streptococcus pneumoniae R6	62.054	98.678	0.612
radA	Streptococcus pneumoniae TIGR4	62.054	98.678	0.612
radA	Streptococcus mitis NCTC 12261	62.054	98.678	0.612
radA	Streptococcus mitis SK321	62.054	98.678	0.612