Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   K8B91_RS05200 Genome accession   NZ_CP082777
Coordinates   1094334..1094774 (+) Length   146 a.a.
NCBI ID   WP_000360915.1    Uniprot ID   A0A0H2V436
Organism   Escherichia coli O25:H1 strain CU39ES-1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1089334..1099774
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K8B91_RS05180 (K8B91_05160) aroP 1090334..1091704 (+) 1371 WP_000399017.1 aromatic amino acid transporter AroP -
  K8B91_RS05185 (K8B91_05165) ampE 1091747..1092601 (-) 855 WP_000172013.1 beta-lactamase regulator AmpE -
  K8B91_RS05190 (K8B91_05170) ampD 1092598..1093149 (-) 552 WP_000923714.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  K8B91_RS05195 (K8B91_05175) nadC 1093237..1094130 (+) 894 WP_001135168.1 carboxylating nicotinate-nucleotide diphosphorylase -
  K8B91_RS05200 (K8B91_05180) pilA 1094334..1094774 (+) 441 WP_000360915.1 prepilin peptidase-dependent pilin Machinery gene
  K8B91_RS05205 (K8B91_05185) pilB 1094784..1096169 (+) 1386 WP_001025180.1 type II secretion system protein GspE Machinery gene
  K8B91_RS05210 (K8B91_05190) hofC 1096159..1097361 (+) 1203 WP_000157242.1 protein transport protein HofC -
  K8B91_RS05215 (K8B91_05195) guaC 1097396..1098439 (-) 1044 WP_001217338.1 GMP reductase -
  K8B91_RS05220 (K8B91_05200) - 1098595..1098639 (-) 45 WP_120795372.1 protein YacM -
  K8B91_RS05225 (K8B91_05205) coaE 1098664..1099284 (+) 621 WP_001269525.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15607.77 Da        Isoelectric Point: 4.5237

>NTDB_id=529019 K8B91_RS05200 WP_000360915.1 1094334..1094774(+) (pilA) [Escherichia coli O25:H1 strain CU39ES-1]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLNTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCTIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=529019 K8B91_RS05200 WP_000360915.1 1094334..1094774(+) (pilA) [Escherichia coli O25:H1 strain CU39ES-1]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAAATACCTGCGACGGTGGCAGCAATGGCATTCCTTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGGCTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGTAACTGCACTATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2V436

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363