Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   K8B92_RS04305 Genome accession   NZ_CP082776
Coordinates   908874..909314 (-) Length   146 a.a.
NCBI ID   WP_000360913.1    Uniprot ID   -
Organism   Escherichia coli O4:H5 strain CU532-9     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 903874..914314
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K8B92_RS04280 (K8B92_04275) coaE 904364..904984 (-) 621 WP_001269524.1 dephospho-CoA kinase -
  K8B92_RS04285 (K8B92_04280) - 905009..905053 (+) 45 WP_120795372.1 protein YacM -
  K8B92_RS04290 (K8B92_04285) guaC 905209..906252 (+) 1044 WP_001217338.1 GMP reductase -
  K8B92_RS04295 (K8B92_04290) hofC 906287..907489 (-) 1203 WP_000157243.1 protein transport protein HofC -
  K8B92_RS04300 (K8B92_04295) pilB 907479..908864 (-) 1386 WP_001535814.1 type II secretion system protein GspE Machinery gene
  K8B92_RS04305 (K8B92_04300) pilA 908874..909314 (-) 441 WP_000360913.1 prepilin peptidase-dependent pilin Machinery gene
  K8B92_RS04310 (K8B92_04305) nadC 909518..910411 (-) 894 WP_001135168.1 carboxylating nicotinate-nucleotide diphosphorylase -
  K8B92_RS04315 (K8B92_04310) ampD 910499..911050 (+) 552 WP_000923726.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  K8B92_RS04320 (K8B92_04315) ampE 911047..911901 (+) 855 WP_000171984.1 beta-lactamase regulator AmpE -
  K8B92_RS04325 (K8B92_04320) aroP 911944..913314 (-) 1371 WP_000399017.1 aromatic amino acid transporter AroP -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15634.80 Da        Isoelectric Point: 4.5237

>NTDB_id=528935 K8B92_RS04305 WP_000360913.1 908874..909314(-) (pilA) [Escherichia coli O4:H5 strain CU532-9]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLNTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSIVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=528935 K8B92_RS04305 WP_000360913.1 908874..909314(-) (pilA) [Escherichia coli O4:H5 strain CU532-9]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAAATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGGCTAAGCATCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGTAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363