Detailed information NTG0528906

Bioinformatically predicted

Overview

• Name: amiE
• Type: Regulator
• Locus tag: K8B89_RS23750
• Genome accession: NZ_CP082775
• Coordinates: 4798741..4799724 (-)
• Length: 327 a.a.
• NCBI ID: WP_001196477.1
• Uniprot ID: A0A0H2VBW7
• Organism: Escherichia coli O25:H4 strain CU568-3
• Function: internalize XIP (predicted from homology) Competence regulation

Genomic Context

Location: 4793741..4804724

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
K8B89_RS23725 (K8B89_23565)	-	4794890..4794997 (-)	108	WP_001295224.1	type I toxin-antitoxin system toxin Ldr family protein	-
K8B89_RS23730 (K8B89_23570)	-	4795373..4795480 (-)	108	WP_000170745.1	type I toxin-antitoxin system toxin Ldr family protein	-
K8B89_RS23735 (K8B89_23575)	-	4795856..4795963 (-)	108	WP_001295224.1	type I toxin-antitoxin system toxin Ldr family protein	-
K8B89_RS23740 (K8B89_23580)	yhjV	4796439..4797710 (+)	1272	WP_001296513.1	aromatic amino acid transport family protein	-
K8B89_RS23745 (K8B89_23585)	dppF	4797740..4798744 (-)	1005	WP_000103571.1	dipeptide ABC transporter ATP-binding subunit DppF	-
K8B89_RS23750 (K8B89_23590)	amiE	4798741..4799724 (-)	984	WP_001196477.1	dipeptide ABC transporter ATP-binding protein	Regulator
K8B89_RS23755 (K8B89_23595)	dppC	4799735..4800637 (-)	903	WP_000084677.1	dipeptide ABC transporter permease DppC	-
K8B89_RS23760 (K8B89_23600)	dppB	4800647..4801666 (-)	1020	WP_000938855.1	dipeptide ABC transporter permease DppB	-
K8B89_RS23765 (K8B89_23605)	dppA	4801973..4803580 (-)	1608	WP_001222871.1	dipeptide ABC transporter substrate-binding protein DppA	-

Sequence

Protein

• Length: 327 a.a.
• Molecular weight: 35801.38 Da
• Isoelectric Point: 6.5813

>NTDB_id=528906 K8B89_RS23750 WP_001196477.1 4798741..4799724(-) (amiE) [Escherichia coli O25:H4 strain CU568-3]
MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGSGKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISE
KERRNLVGAEVAMIFQDPMTSLNPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNLVGIPDPASRLDVYPHQLSGGMSQ
RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVAEAAHKIIVMYAGQVVETGDAHA
IFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPGKYDRPNGCLLNPRCPYATDKCRAEEPALNMLADGRQSKCHYPLD
DAGRPTL

Nucleotide

• Length: 984 bp

>NTDB_id=528906 K8B89_RS23750 WP_001196477.1 4798741..4799724(-) (amiE) [Escherichia coli O25:H4 strain CU568-3]
ATGGCGTTATTAAATGTAGATAAATTATCGGTGCATTTCGGCGACGAAAGCGCACCGTTCCGCGCTGTAGACCGCATCAG
CTACAGCGTAAAACAGGGTGAAGTGGTCGGGATTGTGGGTGAGTCCGGCTCTGGTAAATCGGTCAGTTCGCTGGCGATTA
TGGGGCTGATTGATTATCCGGGCCGCGTAATGGCGGAAAAGCTGGAGTTTAACGGTCAGGATTTGCAGCGTATCTCAGAA
AAAGAGCGCCGCAACCTGGTGGGTGCCGAAGTGGCAATGATCTTCCAGGACCCGATGACCAGCCTTAACCCGTGCTACAC
CGTGGGTTTCCAGATTATGGAAGCGATTAAGGTGCATCAAGGCGGCAACAAAAGTACCCGCCGTCAGCGAGCGATTGACC
TGCTGAATCTGGTCGGTATTCCCGATCCGGCATCGCGTCTGGATGTTTACCCGCATCAGCTTTCCGGCGGCATGAGCCAG
CGCGTGATGATCGCCATGGCGATTGCCTGTCGGCCAAAACTGCTGATTGCCGATGAACCAACCACTGCGCTGGACGTGAC
CATTCAGGCGCAAATTATCGAACTGCTGCTGGAGCTACAGCAGAAAGAGAACATGGCGCTGGTGTTAATTACCCATGACC
TGGCGCTGGTGGCGGAAGCGGCACATAAAATTATCGTGATGTATGCAGGCCAGGTAGTAGAAACGGGCGATGCGCACGCT
ATCTTCCATGCGCCGCGTCACCCGTATACTCAGGCATTGCTGCGTGCGCTGCCGGAATTTGCTCAGGACAAAGAACGTCT
GGCGTCGTTGCCAGGTGTCGTTCCCGGCAAGTACGACCGCCCGAACGGCTGCCTGCTTAACCCGCGCTGCCCCTACGCCA
CTGACAAATGCCGTGCTGAAGAACCGGCGCTGAATATGCTCGCTGACGGGCGTCAGTCCAAATGCCATTACCCACTTGAT
GATGCCGGGAGGCCGACACTATGA

Domains

Predicted by InterProScan.

• ABC transporter (ABC_tran) (24-181)

• Oligopeptide/dipeptide transporter, C-terminal region (oligo_HPY) (233-297)

3D structure

• AlphaFold DB: A0A0H2VBW7

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
amiE	Streptococcus salivarius strain HSISS4	43.302	98.165	0.425
amiE	Streptococcus thermophilus LMG 18311	42.991	98.165	0.422
amiE	Streptococcus thermophilus LMD-9	42.991	98.165	0.422
oppD	Streptococcus mutans UA159	41.379	97.554	0.404