Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   K8B89_RS23300 Genome accession   NZ_CP082775
Coordinates   4697599..4699095 (-) Length   498 a.a.
NCBI ID   WP_001040681.1    Uniprot ID   -
Organism   Escherichia coli O25:H4 strain CU568-3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4692599..4704095
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K8B89_RS23275 (K8B89_23115) ataR 4692989..4693255 (-) 267 WP_001277107.1 type II toxin-antitoxin system antitoxin AtaR -
  K8B89_RS23280 (K8B89_23120) livJ 4693404..4694507 (-) 1104 WP_001021994.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
  K8B89_RS23285 (K8B89_23125) rpoH 4694778..4695632 (-) 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
  K8B89_RS23290 (K8B89_23130) ftsX 4695877..4696935 (-) 1059 WP_001042003.1 permease-like cell division protein FtsX -
  K8B89_RS23295 (K8B89_23135) ftsE 4696928..4697596 (-) 669 WP_000617723.1 cell division ATP-binding protein FtsE -
  K8B89_RS23300 (K8B89_23140) pilA 4697599..4699095 (-) 1497 WP_001040681.1 signal recognition particle-docking protein FtsY Machinery gene
  K8B89_RS23305 (K8B89_23145) rsmD 4699245..4699841 (+) 597 WP_000743189.1 16S rRNA (guanine(966)-N(2))-methyltransferase -
  K8B89_RS23310 (K8B89_23150) yhhL 4699831..4700100 (+) 270 WP_001295207.1 DUF1145 family protein -
  K8B89_RS23315 (K8B89_23155) yhhM 4700103..4700462 (-) 360 WP_000042886.1 DUF2500 domain-containing protein -
  K8B89_RS23320 (K8B89_23160) yhhN 4700603..4701229 (+) 627 WP_000964718.1 lysoplasmalogenase -
  K8B89_RS23325 (K8B89_23165) zntA 4701303..4703501 (+) 2199 WP_000106599.1 Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase ZntA -
  K8B89_RS23330 (K8B89_23170) tusA 4703603..4703848 (-) 246 WP_000130621.1 sulfurtransferase TusA -

Sequence

Protein


Download         Length: 498 a.a.        Molecular weight: 54672.49 Da        Isoelectric Point: 4.1449

>NTDB_id=528903 K8B89_RS23300 WP_001040681.1 4697599..4699095(-) (pilA) [Escherichia coli O25:H4 strain CU568-3]
MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQEQEPVKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVAE
SEKAQPEAEVVAQPEPVVEETPEPVAIEREELPLPEDVNAEAVSPEEWQAEAETVEIVEAAEEEAAKEEITDEEPEAQAL
AAEVAEEAVMVVSPAEEDQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISLFRGKKIDDDLFEELEEQLLIAD
VGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEMGEILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQ
QGKSVMLAAGDTFRAAAVEQLQVWGLRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKSHLMEELKK
IVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLDGTAKGGVIFSVADQFGIPIRYIGVGERIED
LRPFKADDFIEALFARED

Nucleotide


Download         Length: 1497 bp        

>NTDB_id=528903 K8B89_RS23300 WP_001040681.1 4697599..4699095(-) (pilA) [Escherichia coli O25:H4 strain CU568-3]
ATGGCGAAAGAAAAAAAACGTGGCTTTTTTTCCTGGCTGGGCTTTGGTCAAAAAGAGCAGACCCCGGAAAAAGAGACAGA
AGTTCAGAATGAACAACCAGTTGTAGAAGAAATCGTTCAGGAGCAAGAGCCTGTGAAGGCTTCTGAACAAGCCGTTGAAG
AGCAGCCGCAGGCGCATACTGAAGCCGAGGCGGAAACTTTTGCTGCCGACGTTGTGGAAGTTACTGAACAGGTTGCTGAA
AGTGAAAAAGCGCAGCCTGAAGCGGAAGTCGTTGCACAGCCGGAACCGGTCGTAGAAGAAACGCCGGAGCCAGTGGCTAT
CGAACGTGAAGAACTGCCGTTGCCGGAAGACGTCAACGCCGAAGCGGTTTCGCCAGAAGAGTGGCAGGCCGAAGCGGAAA
CCGTAGAGATTGTCGAAGCGGCGGAAGAAGAAGCGGCTAAAGAAGAAATCACCGACGAAGAGCCGGAGGCACAGGCGCTG
GCTGCCGAAGTGGCAGAAGAGGCGGTAATGGTGGTTTCTCCGGCAGAAGAAGATCAGCCGGTGGAAGAAATCGCTCAGGA
GCAGGAAAAACCGACCAAAGAAGGTTTCTTCGCGCGCCTGAAACGCAGCCTGTTAAAAACCAAAGAAAATCTCGGTTCCG
GATTTATCAGCCTGTTCCGCGGTAAAAAAATCGACGATGATCTGTTTGAAGAGCTGGAAGAACAGCTGTTGATCGCCGAT
GTGGGCGTGGAAACCACTCGTAAAATTATCACCAATCTGACAGAAGGCGCATCCCGCAAGCAGCTTCGTGATGCCGAGGC
GCTCTATGGCCTGCTGAAAGAAGAGATGGGCGAGATTCTGGCGAAAGTCGATGAGCCGCTGAATGTTGAAGGTAAAACGC
CGTTCGTGATCCTGATGGTGGGCGTCAACGGTGTGGGTAAAACCACGACGATTGGTAAGCTGGCGCGTCAGTTTGAGCAG
CAGGGTAAATCGGTGATGCTGGCGGCAGGCGATACTTTCCGTGCGGCAGCGGTTGAACAGCTTCAGGTCTGGGGTCTGCG
CAACAATATTCCGGTGATTGCCCAGCATACCGGTGCGGATTCCGCTTCTGTTATCTTCGATGCCATTCAGGCCGCTAAAG
CGCGTAACATCGACGTCCTGATTGCCGATACCGCCGGACGCCTGCAAAACAAATCGCACCTGATGGAAGAGTTGAAGAAA
ATCGTCCGCGTGATGAAGAAACTCGACGTTGAAGCTCCGCATGAAGTTATGCTGACCATCGATGCCAGCACCGGGCAGAA
TGCGGTAAGCCAGGCCAAACTGTTCCACGAAGCCGTTGGCTTAACCGGCATCACGCTAACGAAACTGGACGGCACGGCGA
AAGGCGGGGTAATTTTCTCGGTGGCTGACCAGTTTGGTATCCCTATCCGCTACATTGGTGTCGGCGAACGTATTGAGGAT
TTGCGTCCGTTTAAGGCGGACGACTTTATAGAGGCACTTTTTGCCCGAGAGGATTAA

Domains

Predicted by InterProScan.

SRP54-type protein, GTPase domain (SRP54)

(294-494)

SRP54-type protein, helical bundle domain (SRP54_N)

(211-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

48.831

77.309

0.378