Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   K8B87_RS22015 Genome accession   NZ_CP082773
Coordinates   4564504..4566000 (-) Length   498 a.a.
NCBI ID   WP_061336480.1    Uniprot ID   -
Organism   Escherichia coli O99:H6 strain CU42ET-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4559504..4571000
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K8B87_RS21990 (K8B87_21940) panM 4559738..4560121 (+) 384 WP_000778796.1 aspartate 1-decarboxylase autocleavage activator PanM -
  K8B87_RS21995 (K8B87_21945) livJ 4560309..4561412 (-) 1104 WP_001350438.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
  K8B87_RS22000 (K8B87_21950) rpoH 4561683..4562537 (-) 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
  K8B87_RS22005 (K8B87_21955) ftsX 4562782..4563840 (-) 1059 WP_001042003.1 permease-like cell division protein FtsX -
  K8B87_RS22010 (K8B87_21960) ftsE 4563833..4564501 (-) 669 WP_000617723.1 cell division ATP-binding protein FtsE -
  K8B87_RS22015 (K8B87_21965) pilA 4564504..4566000 (-) 1497 WP_061336480.1 signal recognition particle-docking protein FtsY Machinery gene
  K8B87_RS22020 (K8B87_21970) rsmD 4566150..4566746 (+) 597 WP_000743193.1 16S rRNA (guanine(966)-N(2))-methyltransferase -
  K8B87_RS22025 (K8B87_21975) yhhL 4566736..4567005 (+) 270 WP_001295207.1 DUF1145 family protein -
  K8B87_RS22030 (K8B87_21980) yhhM 4567008..4567367 (-) 360 WP_000042886.1 DUF2500 domain-containing protein -
  K8B87_RS22035 (K8B87_21985) yhhN 4567508..4568134 (+) 627 WP_000964718.1 lysoplasmalogenase -
  K8B87_RS22040 (K8B87_21990) zntA 4568208..4570400 (+) 2193 WP_087602848.1 Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase ZntA -

Sequence

Protein


Download         Length: 498 a.a.        Molecular weight: 54641.44 Da        Isoelectric Point: 4.1849

>NTDB_id=528730 K8B87_RS22015 WP_061336480.1 4564504..4566000(-) (pilA) [Escherichia coli O99:H6 strain CU42ET-1]
MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEPVKASEHAVEEQPQAHTEAEAETFAANVVEVTEQVAE
SEKAQPEAEVVAQPESVVEETPEPVAIEREELPLPEDVNAEAVSPEEWQAEAETVEIVEAAEEEAAKEEITDEEPEAQAL
AAEVAEEAVMVVSPAEEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISLFRGKKIDDDLFEELEEQLLIAD
VGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEMGEILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQ
QGKSVMLAAGDTFRAAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKSHLMEELKK
IVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLDGTAKGGVIFSVADQFGIPIRYIGVGERIED
LRPFKADDFIEALFARED

Nucleotide


Download         Length: 1497 bp        

>NTDB_id=528730 K8B87_RS22015 WP_061336480.1 4564504..4566000(-) (pilA) [Escherichia coli O99:H6 strain CU42ET-1]
ATGGCGAAAGAAAAAAAACGTGGCTTTTTTTCCTGGCTGGGCTTTGGTCAAAAAGAGCAGACCCCGGAAAAAGAGACAGA
AGTTCAGAATGAACAACCAGTTGTAGAAGAAATCGTTCAGGCGCAAGAGCCTGTGAAGGCTTCTGAACACGCCGTTGAAG
AGCAGCCGCAGGCGCATACTGAAGCCGAGGCGGAAACTTTTGCTGCCAACGTTGTGGAAGTTACTGAACAGGTTGCTGAA
AGTGAAAAAGCGCAGCCTGAAGCGGAAGTCGTTGCACAGCCGGAATCGGTCGTAGAAGAAACGCCGGAGCCAGTGGCTAT
CGAACGTGAAGAACTGCCGTTGCCGGAAGACGTCAACGCCGAAGCGGTTTCGCCAGAAGAGTGGCAGGCCGAAGCGGAAA
CCGTAGAGATTGTCGAAGCGGCGGAAGAAGAAGCGGCTAAAGAAGAAATCACCGACGAAGAGCCGGAGGCACAGGCGCTG
GCTGCCGAAGTGGCAGAAGAGGCGGTAATGGTGGTTTCTCCGGCAGAAGAAGAGCAGCCGGTGGAAGAAATCGCTCAGGA
GCAGGAAAAACCGACCAAAGAAGGTTTCTTCGCGCGCCTGAAACGCAGCCTGTTAAAAACCAAAGAAAATCTCGGTTCCG
GATTTATCAGCCTGTTCCGCGGTAAAAAAATCGACGATGATCTGTTTGAAGAGCTGGAAGAACAGCTGTTGATCGCCGAT
GTGGGCGTGGAAACCACTCGTAAAATTATCACCAATCTGACGGAAGGCGCATCCCGCAAGCAGCTTCGTGATGCCGAGGC
GCTCTATGGCCTGCTGAAAGAAGAGATGGGCGAGATTCTGGCGAAAGTCGATGAGCCGCTGAATGTTGAAGGTAAAACGC
CGTTCGTGATCCTGATGGTGGGCGTCAACGGTGTGGGTAAAACCACGACGATTGGTAAGCTGGCGCGTCAGTTTGAGCAG
CAGGGTAAATCGGTGATGCTGGCGGCAGGCGATACTTTCCGTGCGGCAGCGGTTGAACAGCTTCAGGTCTGGGGGCAGCG
CAACAATATTCCGGTGATTGCCCAGCATACCGGTGCGGATTCCGCTTCTGTTATCTTCGACGCCATTCAGGCCGCTAAAG
CGCGTAACATCGACGTCCTGATTGCCGATACCGCCGGACGCCTGCAGAACAAATCGCACCTGATGGAAGAGTTGAAGAAA
ATCGTCCGCGTGATGAAGAAACTCGACGTTGAAGCTCCGCATGAAGTTATGCTGACTATTGATGCCAGCACCGGGCAGAA
TGCGGTAAGCCAGGCCAAACTGTTCCACGAAGCCGTTGGCTTAACCGGCATCACGCTAACGAAACTGGACGGCACGGCGA
AAGGCGGGGTAATTTTCTCGGTGGCTGACCAGTTTGGTATCCCTATCCGCTACATTGGTGTCGGCGAACGTATTGAGGAT
TTGCGTCCGTTTAAGGCGGACGACTTTATAGAGGCACTTTTTGCCCGAGAGGATTAA

Domains

Predicted by InterProScan.

SRP54-type protein, GTPase domain (SRP54)

(294-494)

SRP54-type protein, helical bundle domain (SRP54_N)

(211-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

44.889

90.361

0.406