Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   JK151_RS03445 Genome accession   NZ_CP068285
Coordinates   732601..733728 (+) Length   375 a.a.
NCBI ID   WP_013213631.1    Uniprot ID   A0A1U9VHA8
Organism   Ralstonia syzygii subsp. celebesensis strain UGMSS_Db01     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 727601..738728
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JK151_RS03420 (JK151_03420) - 727976..728224 (-) 249 WP_078222383.1 YfhL family 4Fe-4S dicluster ferredoxin -
  JK151_RS03425 (JK151_03425) coaD 728275..728781 (-) 507 WP_003262550.1 pantetheine-phosphate adenylyltransferase -
  JK151_RS03430 (JK151_03430) rsmD 728977..729624 (-) 648 WP_075465409.1 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD -
  JK151_RS03435 (JK151_03435) - 729704..731047 (-) 1344 WP_013213629.1 M16 family metallopeptidase -
  JK151_RS03440 (JK151_03440) - 731047..732540 (-) 1494 WP_078222382.1 M16 family metallopeptidase -
  JK151_RS03445 (JK151_03445) pilA 732601..733728 (+) 1128 WP_013213631.1 signal recognition particle-docking protein FtsY Machinery gene
  JK151_RS03450 (JK151_03450) - 733725..734363 (-) 639 WP_078222381.1 LysE/ArgO family amino acid transporter -
  JK151_RS03455 (JK151_03455) maiA 734360..735010 (-) 651 WP_078222380.1 maleylacetoacetate isomerase -
  JK151_RS03460 (JK151_03460) - 735014..735715 (-) 702 WP_078222379.1 fumarylacetoacetate hydrolase family protein -
  JK151_RS03465 (JK151_03465) - 735866..736492 (+) 627 WP_013213635.1 ANTAR domain-containing response regulator -
  JK151_RS03470 (JK151_03470) - 736769..738031 (+) 1263 WP_078222378.1 CmpA/NrtA family ABC transporter substrate-binding protein -

Sequence

Protein


Download         Length: 375 a.a.        Molecular weight: 39819.04 Da        Isoelectric Point: 6.1635

>NTDB_id=528723 JK151_RS03445 WP_013213631.1 732601..733728(+) (pilA) [Ralstonia syzygii subsp. celebesensis strain UGMSS_Db01]
MFSFWKKRKAEPQPAAEPVPPAAAPEAVQAPAPAPVASPAPAPAPVAVPDTPATPAALDMQADDIETVPTPPVVEQARKG
WMSRLRSGLSKTSKGLTTLFVGVKVDEALFEELETALLMADAGVDATEYLLGELRRRVKAQRIETAEGVKTALRDLLIEL
LHPLEKTMVLGREQPTVIMIAGVNGAGKTTSIGKLCKHFQTYGQSVLLAAGDTFRAAAREQLVIWGQRNNVTVVAQESGD
PAAVIFDAVNAARARGIDIVMADTAGRLPTQLHLMEELKKVRRVIGKAMATAPHETLLVIDANTGQNALAQVKAFDDTLG
LTGLIITKLDGTAKGGILAAIARQRPVPVYFIGVGEQVEDLQPFSAREFADALLG

Nucleotide


Download         Length: 1128 bp        

>NTDB_id=528723 JK151_RS03445 WP_013213631.1 732601..733728(+) (pilA) [Ralstonia syzygii subsp. celebesensis strain UGMSS_Db01]
ATGTTTAGTTTCTGGAAGAAGCGCAAGGCAGAGCCCCAGCCGGCTGCCGAACCGGTGCCGCCCGCCGCCGCACCTGAAGC
GGTACAGGCTCCAGCCCCGGCCCCGGTTGCCTCCCCTGCACCTGCACCCGCCCCGGTTGCCGTGCCGGATACACCGGCCA
CGCCGGCTGCGCTGGACATGCAGGCCGACGATATCGAGACCGTGCCGACGCCGCCCGTGGTGGAGCAGGCGCGCAAGGGC
TGGATGTCGCGCCTGCGCTCGGGCCTGTCGAAGACCAGCAAGGGCCTGACCACGCTGTTCGTCGGCGTGAAGGTGGACGA
GGCGCTGTTCGAGGAGCTGGAAACCGCGCTGCTGATGGCCGATGCAGGCGTCGATGCCACCGAATACCTGCTGGGCGAAC
TGCGCCGCCGTGTGAAAGCCCAGCGCATCGAGACCGCCGAGGGCGTGAAGACCGCGCTGCGCGACCTGCTCATCGAGCTG
CTGCATCCGCTGGAAAAGACCATGGTGCTCGGCCGCGAGCAGCCGACGGTGATCATGATCGCGGGCGTCAACGGCGCCGG
CAAGACCACCAGCATCGGCAAGCTGTGCAAGCACTTCCAGACCTACGGGCAATCCGTGCTGCTGGCAGCGGGCGACACGT
TCCGTGCTGCCGCGCGCGAGCAGTTGGTGATCTGGGGCCAGCGCAACAACGTGACCGTGGTGGCGCAGGAATCGGGCGAC
CCGGCCGCCGTGATCTTCGACGCGGTGAACGCGGCGCGCGCCCGCGGCATCGATATCGTCATGGCCGACACCGCCGGCCG
CCTGCCGACCCAGCTGCACCTGATGGAAGAACTGAAGAAGGTGCGCCGCGTGATCGGCAAGGCGATGGCGACGGCGCCGC
ACGAGACGTTGCTGGTGATCGACGCCAACACCGGCCAGAATGCGCTGGCGCAGGTGAAAGCCTTCGACGATACGCTCGGC
CTGACCGGCCTGATCATCACCAAGCTGGACGGCACCGCCAAGGGCGGCATCCTGGCCGCCATCGCGCGGCAGCGGCCTGT
ACCGGTGTATTTCATCGGCGTGGGCGAACAGGTGGAAGACCTGCAGCCGTTCTCCGCACGCGAATTCGCCGACGCGCTAC
TGGGCTAG

Domains

Predicted by InterproScan.

SRP54-type protein, GTPase domain (SRP54)

(175-375)

SRP54-type protein, helical bundle domain (SRP54_N)

(94-155)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1U9VHA8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

62.951

81.333

0.512