Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   K8B87_RS04445 Genome accession   NZ_CP082773
Coordinates   942893..943333 (-) Length   146 a.a.
NCBI ID   WP_000360913.1    Uniprot ID   -
Organism   Escherichia coli O99:H6 strain CU42ET-1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 937893..948333
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K8B87_RS04420 (K8B87_04420) coaE 938383..939003 (-) 621 WP_001269527.1 dephospho-CoA kinase -
  K8B87_RS04425 (K8B87_04425) - 939028..939072 (+) 45 WP_120795372.1 protein YacM -
  K8B87_RS04430 (K8B87_04430) guaC 939228..940271 (+) 1044 WP_001217338.1 GMP reductase -
  K8B87_RS04435 (K8B87_04435) hofC 940306..941508 (-) 1203 WP_000157289.1 protein transport protein HofC -
  K8B87_RS04440 (K8B87_04440) pilB 941498..942883 (-) 1386 WP_001025178.1 type II secretion system protein GspE Machinery gene
  K8B87_RS04445 (K8B87_04445) pilA 942893..943333 (-) 441 WP_000360913.1 prepilin peptidase-dependent pilin Machinery gene
  K8B87_RS04450 (K8B87_04450) nadC 943537..944430 (-) 894 WP_001135168.1 carboxylating nicotinate-nucleotide diphosphorylase -
  K8B87_RS04455 (K8B87_04455) ampD 944518..945069 (+) 552 WP_000923703.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  K8B87_RS04460 (K8B87_04460) ampE 945066..945920 (+) 855 WP_000172010.1 beta-lactamase regulator AmpE -
  K8B87_RS04465 (K8B87_04465) aroP 945963..947333 (-) 1371 WP_000399047.1 aromatic amino acid transporter AroP -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15634.80 Da        Isoelectric Point: 4.5237

>NTDB_id=528673 K8B87_RS04445 WP_000360913.1 942893..943333(-) (pilA) [Escherichia coli O99:H6 strain CU42ET-1]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLNTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSIVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=528673 K8B87_RS04445 WP_000360913.1 942893..943333(-) (pilA) [Escherichia coli O99:H6 strain CU42ET-1]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAAATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGGCTAAGCATCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGTAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363