Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   ABCA12_RS13145 Genome accession   NZ_CP068253
Coordinates   2758484..2759284 (-) Length   266 a.a.
NCBI ID   WP_004908991.1    Uniprot ID   -
Organism   Acinetobacter junii strain CAM121     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2753484..2764284
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABCA12_RS13125 (ABCA12_2692) - 2754543..2755241 (-) 699 WP_004950316.1 YfgM family protein -
  ABCA12_RS13130 (ABCA12_2693) hisS 2755273..2756565 (-) 1293 WP_039047056.1 histidine--tRNA ligase -
  ABCA12_RS13135 (ABCA12_2694) ispG 2756562..2757677 (-) 1116 WP_004908985.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  ABCA12_RS13140 (ABCA12_2695) - 2757702..2758493 (-) 792 WP_201897789.1 helix-turn-helix domain-containing protein -
  ABCA12_RS13145 (ABCA12_2696) pilF 2758484..2759284 (-) 801 WP_004908991.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  ABCA12_RS13150 (ABCA12_2697) rlmN 2759301..2760536 (-) 1236 WP_042891255.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  ABCA12_RS13155 (ABCA12_2698) ndk 2760658..2761089 (-) 432 WP_201897790.1 nucleoside-diphosphate kinase -
  ABCA12_RS13160 (ABCA12_2699) iscX 2761196..2761396 (-) 201 WP_004652532.1 Fe-S cluster assembly protein IscX -
  ABCA12_RS13165 (ABCA12_2700) - 2761479..2762030 (-) 552 WP_201897791.1 phosphatase PAP2 family protein -
  ABCA12_RS13170 (ABCA12_2701) - 2762058..2763374 (-) 1317 WP_201897792.1 glycosyltransferase family 4 protein -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 30366.44 Da        Isoelectric Point: 9.5600

>NTDB_id=528637 ABCA12_RS13145 WP_004908991.1 2758484..2759284(-) (pilF) [Acinetobacter junii strain CAM121]
MNTTRLKTILATTVVFTVLGLTACQSTTLRKDPEKAVQVRTQLAAEYIRTRDLDSAKRSLDQALKIDPRDATANMMMGVL
LQQEGSKPNMEKAEAYFKRAISSEPDNAQARNNYGTYLYQMQRYNEAIEQFTVAGATLGYEQRYQSLENLGRIYLKLGDV
ANAEKSFKQALQANRNSTISMLELSEIFYLQQNNRDASQLYEQYVRNVGQKNQGARALWIGIRIARANADQLGMQVLVNQ
MRALFPDSPEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 801 bp        

>NTDB_id=528637 ABCA12_RS13145 WP_004908991.1 2758484..2759284(-) (pilF) [Acinetobacter junii strain CAM121]
TTGAATACAACTCGACTAAAAACGATTTTAGCTACTACTGTTGTGTTTACTGTATTAGGTTTGACCGCCTGCCAATCAAC
AACGTTAAGAAAAGATCCAGAAAAGGCAGTACAAGTCCGAACTCAACTCGCTGCTGAATATATTCGAACACGTGATTTAG
ATTCAGCGAAACGCTCCCTTGATCAAGCTTTAAAAATTGATCCACGTGATGCTACTGCCAATATGATGATGGGTGTTTTG
TTGCAACAAGAAGGCAGCAAACCAAACATGGAGAAAGCAGAAGCTTATTTTAAGCGAGCAATCTCTTCAGAACCCGATAA
TGCCCAAGCTCGTAATAATTACGGTACATATTTGTATCAAATGCAACGTTATAATGAGGCGATTGAGCAATTTACCGTTG
CAGGTGCGACGTTAGGTTACGAACAGCGTTACCAATCGTTGGAAAACTTGGGTCGAATCTATTTAAAACTGGGTGATGTT
GCGAATGCTGAAAAATCATTCAAGCAAGCCTTACAAGCAAATCGAAATTCGACAATTTCAATGTTAGAGTTGTCCGAAAT
TTTCTATTTGCAACAAAATAATAGAGATGCATCGCAACTTTATGAACAATACGTTCGTAATGTCGGGCAGAAAAATCAAG
GTGCTCGTGCACTTTGGATCGGTATACGCATTGCTCGAGCGAATGCGGATCAATTAGGAATGCAAGTATTAGTGAATCAA
ATGCGTGCTTTATTTCCTGATAGTCCAGAATATCAACGTTATTTGCAATTACAGTACAGTACTGAGGCCGTATGGAAATA
A

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(39-69)

Tetratricopeptide repeat (TPR_8)

(145-176)

Tetratricopeptide repeat (TPR_16)

(90-134)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

79.026

100

0.793
  pilF Acinetobacter baylyi ADP1

68.045

100

0.68