Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   K8B93_RS04725 Genome accession   NZ_CP082772
Coordinates   1014556..1014996 (-) Length   146 a.a.
NCBI ID   WP_000360915.1    Uniprot ID   A0A0H2V436
Organism   Escherichia coli O2:H1 strain HM670     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1009556..1019996
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K8B93_RS04700 (K8B93_04690) coaE 1010046..1010666 (-) 621 WP_001269525.1 dephospho-CoA kinase -
  K8B93_RS04705 (K8B93_04695) - 1010691..1010735 (+) 45 WP_120795372.1 protein YacM -
  K8B93_RS04710 (K8B93_04700) guaC 1010891..1011934 (+) 1044 WP_001217338.1 GMP reductase -
  K8B93_RS04715 (K8B93_04705) hofC 1011969..1013171 (-) 1203 WP_000157242.1 protein transport protein HofC -
  K8B93_RS04720 (K8B93_04710) pilB 1013161..1014546 (-) 1386 WP_001025180.1 type II secretion system protein GspE Machinery gene
  K8B93_RS04725 (K8B93_04715) pilA 1014556..1014996 (-) 441 WP_000360915.1 prepilin peptidase-dependent pilin Machinery gene
  K8B93_RS04730 (K8B93_04720) nadC 1015200..1016093 (-) 894 WP_001135168.1 carboxylating nicotinate-nucleotide diphosphorylase -
  K8B93_RS04735 (K8B93_04725) ampD 1016181..1016732 (+) 552 WP_000923726.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  K8B93_RS04740 (K8B93_04730) ampE 1016729..1017583 (+) 855 WP_234287575.1 beta-lactamase regulator AmpE -
  K8B93_RS04745 (K8B93_04735) aroP 1017626..1018996 (-) 1371 WP_000399017.1 aromatic amino acid transporter AroP -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15607.77 Da        Isoelectric Point: 4.5237

>NTDB_id=528586 K8B93_RS04725 WP_000360915.1 1014556..1014996(-) (pilA) [Escherichia coli O2:H1 strain HM670]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLNTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCTIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=528586 K8B93_RS04725 WP_000360915.1 1014556..1014996(-) (pilA) [Escherichia coli O2:H1 strain HM670]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAAATACCTGCGACGGTGGCAGCAATGGCATTCCTTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGGCTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGTAACTGCACTATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2V436

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363