Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   K8B90_RS21595 Genome accession   NZ_CP082771
Coordinates   4448995..4450491 (-) Length   498 a.a.
NCBI ID   WP_001040639.1    Uniprot ID   B7UL15
Organism   Escherichia coli LF82     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4443995..4455491
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K8B90_RS21570 (K8B90_21470) panM 4444229..4444612 (+) 384 WP_000778808.1 aspartate 1-decarboxylase autocleavage activator PanM -
  K8B90_RS21575 (K8B90_21475) livJ 4444800..4445903 (-) 1104 WP_001021996.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
  K8B90_RS21580 (K8B90_21480) rpoH 4446174..4447028 (-) 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
  K8B90_RS21585 (K8B90_21485) ftsX 4447273..4448331 (-) 1059 WP_001042003.1 permease-like cell division protein FtsX -
  K8B90_RS21590 (K8B90_21490) ftsE 4448324..4448992 (-) 669 WP_000617723.1 cell division ATP-binding protein FtsE -
  K8B90_RS21595 (K8B90_21495) pilA 4448995..4450491 (-) 1497 WP_001040639.1 signal recognition particle-docking protein FtsY Machinery gene
  K8B90_RS21600 (K8B90_21500) rsmD 4450641..4451237 (+) 597 WP_000743193.1 16S rRNA (guanine(966)-N(2))-methyltransferase -
  K8B90_RS21605 (K8B90_21505) yhhL 4451227..4451496 (+) 270 WP_001295207.1 DUF1145 family protein -
  K8B90_RS21610 (K8B90_21510) yhhM 4451499..4451858 (-) 360 WP_000042886.1 DUF2500 domain-containing protein -
  K8B90_RS21615 (K8B90_21515) yhhN 4451999..4452625 (+) 627 WP_000964718.1 lysoplasmalogenase -
  K8B90_RS21620 (K8B90_21520) zntA 4452699..4454897 (+) 2199 WP_000106590.1 Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase ZntA -
  K8B90_RS21625 (K8B90_21525) tusA 4455158..4455403 (-) 246 WP_000130621.1 sulfurtransferase TusA -

Sequence

Protein


Download         Length: 498 a.a.        Molecular weight: 54624.41 Da        Isoelectric Point: 4.1733

>NTDB_id=528554 K8B90_RS21595 WP_001040639.1 4448995..4450491(-) (pilA) [Escherichia coli LF82]
MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEPVKASEHAVEEQPQAHTEAEAETFAADVVEVTEQVAE
SEKAQPEAEVVAQPEPVVEETPEPVAIEREELPLPEDVNAEAVSPEEWQAEAETVEIVEAAEEEAAKEEITDEEPEAQAL
AAEAAEEAVMVVSPAEEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISLFRGKKIDDDLFEELEEQLLIAD
VGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEMGEILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQ
QGKSVMLAAGDTFRAAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKSHLMEELKK
IVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLDGTAKGGVIFSVADQFGIPIRYIGVGERIED
LRPFKADDFIEALFARED

Nucleotide


Download         Length: 1497 bp        

>NTDB_id=528554 K8B90_RS21595 WP_001040639.1 4448995..4450491(-) (pilA) [Escherichia coli LF82]
ATGGCGAAAGAAAAAAAACGTGGCTTTTTTTCCTGGCTGGGCTTTGGTCAAAAAGAGCAGACCCCGGAAAAAGAGACAGA
AGTTCAGAATGAACAACCAGTTGTAGAAGAAATCGTTCAGGCGCAAGAGCCTGTGAAGGCTTCTGAACACGCCGTTGAAG
AGCAGCCGCAGGCGCATACTGAAGCCGAGGCGGAAACTTTTGCTGCCGACGTTGTGGAAGTTACTGAACAGGTTGCTGAA
AGTGAAAAAGCGCAGCCTGAAGCGGAAGTCGTTGCACAGCCGGAACCGGTCGTAGAAGAAACGCCGGAGCCAGTGGCTAT
CGAACGTGAAGAACTGCCGTTGCCGGAAGACGTCAACGCCGAAGCGGTTTCGCCAGAAGAGTGGCAGGCCGAAGCGGAAA
CCGTAGAGATTGTCGAAGCGGCGGAAGAAGAAGCGGCTAAAGAAGAAATCACCGACGAAGAGCCGGAGGCACAGGCGCTG
GCTGCCGAAGCGGCAGAAGAGGCGGTAATGGTGGTTTCTCCGGCAGAAGAAGAGCAGCCGGTGGAAGAAATCGCTCAGGA
GCAGGAAAAACCGACCAAAGAAGGTTTCTTCGCGCGCCTGAAACGCAGCCTGTTAAAAACCAAAGAAAATCTCGGTTCCG
GATTTATCAGTCTGTTCCGCGGTAAAAAAATCGACGATGATCTGTTTGAAGAGCTGGAAGAACAGCTGTTGATCGCCGAT
GTGGGCGTGGAAACCACACGTAAAATTATCACCAATCTGACGGAAGGCGCATCCCGCAAGCAGCTTCGTGATGCCGAGGC
GCTCTATGGCCTGCTGAAAGAAGAGATGGGCGAGATTCTGGCGAAAGTCGATGAGCCGCTGAATGTTGAAGGTAAAACGC
CGTTCGTGATCCTGATGGTGGGCGTCAACGGTGTGGGTAAAACCACGACGATTGGTAAGCTGGCGCGTCAGTTTGAGCAG
CAGGGTAAATCGGTGATGCTGGCGGCAGGCGATACTTTCCGTGCGGCAGCGGTTGAACAGCTTCAGGTCTGGGGCCAGCG
CAACAATATTCCGGTGATTGCCCAGCATACCGGTGCGGATTCCGCTTCTGTTATCTTCGACGCCATTCAGGCCGCTAAAG
CGCGTAACATCGACGTCCTGATTGCCGATACCGCCGGACGCCTGCAAAACAAATCGCACCTGATGGAAGAGTTGAAGAAA
ATCGTCCGCGTGATGAAGAAACTCGACGTTGAAGCTCCGCATGAAGTTATGCTGACTATTGATGCCAGCACCGGGCAGAA
TGCGGTAAGCCAGGCCAAACTGTTCCACGAAGCCGTTGGCTTAACCGGCATCACGCTAACGAAACTGGACGGCACGGCGA
AAGGCGGGGTAATTTTCTCGGTGGCTGACCAGTTTGGTATCCCTATCCGCTACATTGGTGTCGGCGAACGTATTGAGGAT
TTGCGTCCGTTTAAGGCGGACGACTTTATAGAGGCACTTTTTGCCCGAGAGGATTAA

Domains

Predicted by InterProScan.

SRP54-type protein, GTPase domain (SRP54)

(294-494)

SRP54-type protein, helical bundle domain (SRP54_N)

(211-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7UL15

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

48.831

77.309

0.378