Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA/sms   Type   Machinery gene
Locus tag   K7X33_RS19365 Genome accession   NZ_CP082355
Coordinates   4059539..4060921 (-) Length   460 a.a.
NCBI ID   WP_001029698.1    Uniprot ID   A0A2T3RLH6
Organism   Escherichia coli strain VCSW5     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 4054539..4065921
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K7X33_RS19325 (K7X33_19325) - 4054621..4055418 (+) 798 WP_000762711.1 SDR family NAD(P)-dependent oxidoreductase -
  K7X33_RS19330 (K7X33_19330) - 4055444..4055860 (+) 417 WP_001140838.1 SRPBCC family protein -
  K7X33_RS19335 (K7X33_19335) - 4056018..4056230 (+) 213 WP_000848792.1 DUF1471 domain-containing protein -
  K7X33_RS23210 - 4056303..4056419 (+) 117 Protein_3800 hypothetical protein -
  K7X33_RS23215 - 4056379..4056507 (+) 129 WP_000886305.1 hypothetical protein -
  K7X33_RS19345 (K7X33_19345) - 4056658..4057029 (-) 372 WP_001036747.1 winged helix-turn-helix domain-containing protein -
  K7X33_RS19350 (K7X33_19350) - 4057055..4057861 (-) 807 WP_223040175.1 sulfite exporter TauE/SafE family protein -
  K7X33_RS23375 (K7X33_19355) nadS 4058106..4058252 (-) 147 Protein_3804 NadS family protein -
  K7X33_RS19360 (K7X33_19360) nadR 4058286..4059518 (-) 1233 WP_000093813.1 multifunctional transcriptional regulator/nicotinamide-nucleotide adenylyltransferase/ribosylnicotinamide kinase NadR -
  K7X33_RS19365 (K7X33_19365) radA/sms 4059539..4060921 (-) 1383 WP_001029698.1 DNA repair protein RadA Machinery gene
  K7X33_RS19370 (K7X33_19370) serB 4060970..4061938 (-) 969 WP_001132956.1 phosphoserine phosphatase -
  K7X33_RS19375 (K7X33_19375) ytjB 4062044..4062688 (+) 645 WP_000124615.1 YtjB family periplasmic protein -
  K7X33_RS19380 (K7X33_19380) lplA 4062716..4063732 (+) 1017 WP_000105879.1 lipoate--protein ligase LplA -
  K7X33_RS19385 (K7X33_19385) - 4063764..4064027 (+) 264 WP_000566145.1 helix-turn-helix domain-containing protein -
  K7X33_RS19390 (K7X33_19390) deoD 4064188..4064907 (-) 720 WP_000224879.1 purine-nucleoside phosphorylase -

Sequence

Protein


Download         Length: 460 a.a.        Molecular weight: 49442.02 Da        Isoelectric Point: 7.1936

>NTDB_id=528382 K7X33_RS19365 WP_001029698.1 4059539..4060921(-) (radA/sms) [Escherichia coli strain VCSW5]
MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPTVARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTG
FKEFDRVLGGGVVPGSAILIGGNPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMVGHVTKDGSLAGPKVLEHCIDCS
VLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQGLREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDH
SMMANPRRVAVGLEQNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQDLVVFGEV
GLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKLSDALSVFDDL

Nucleotide


Download         Length: 1383 bp        

>NTDB_id=528382 K7X33_RS19365 WP_001029698.1 4059539..4060921(-) (radA/sms) [Escherichia coli strain VCSW5]
GTGGCAAAAGCTCCAAAACGCGCCTTTGTTTGTAATGAATGCGGGGCCGATTATCCGCGCTGGCAGGGGCAGTGCAGCGC
CTGTCATGCCTGGAACACCATCACCGAGGTGCGTCTTGCAGCGTCGCCAACGGTGGCGCGTAACGAGCGTCTCAGTGGCT
ATGCCGGTAGCGCCGGAGTGGCAAAAGTCCAGAAACTCTCCGACATCAGCCTTGAGGAGCTGCCGCGTTTTTCTACCGGA
TTTAAAGAGTTCGACCGCGTATTAGGCGGCGGGGTGGTGCCGGGAAGCGCCATTCTGATTGGCGGTAACCCAGGCGCGGG
GAAATCCACGCTGCTGTTGCAAACGCTGTGCAAACTGGCCCAGCAGATGAAAACGCTGTATGTCACCGGCGAAGAGTCGC
TGCAACAGGTGGCAATGCGCGCTCATCGCCTTGGCCTGCCGACTGACAATCTCAATATGTTGTCGGAAACCAGCATCGAG
CAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCCATCCAGGTGATGCATATGGCGGATGT
TCAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGCGTGG
CGATTGTCATGGTTGGGCACGTAACCAAAGATGGCTCGCTGGCTGGCCCTAAAGTGCTGGAACACTGTATCGACTGTTCG
GTGCTTCTGGATGGCGATGCCGACTCCCGTTTTCGCACCTTACGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCT
GGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTTAGCAACCCTTCCGCCATCTTTTTAAGCCGCGGTGATGAAG
TGACCTCCGGTAGCTCAGTGATGGTGGTATGGGAAGGAACGCGTCCACTGCTGGTGGAGATCCAGGCGCTGGTCGATCAC
TCGATGATGGCGAATCCACGCCGCGTCGCAGTAGGGCTGGAACAAAACCGTCTGGCAATCCTGCTGGCTGTCTTGCACCG
TCACGGTGGGCTGCAAATGGCCGATCAGGATGTATTTGTGAACGTGGTCGGCGGCGTGAAGGTGACGGAAACCAGTGCCG
ACTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGCGACAGACCGCTGCCGCAGGATCTGGTGGTGTTTGGTGAAGTC
GGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATCTCTGAAGCGGCGAAACACGGTTTTCGGCGGGC
GATTGTTCCGGCGGCTAATGTGCCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGC
TTAGCGTGTTCGACGACTTATAA

Domains

Predicted by InterProScan.

AAA domain (AAA_25)

(89-225)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(9-36)

Subunit ChlI of Mg-chelatase (ChlI)

(348-433)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2T3RLH6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA/sms Bacillus subtilis subsp. subtilis str. 168

46.827

99.348

0.465
  radA Streptococcus mitis SK321

43.86

99.13

0.435
  radA Streptococcus pneumoniae D39

43.421

99.13

0.43
  radA Streptococcus pneumoniae TIGR4

43.421

99.13

0.43
  radA Streptococcus mitis NCTC 12261

43.421

99.13

0.43
  radA Streptococcus pneumoniae R6

43.421

99.13

0.43
  radA Streptococcus pneumoniae Rx1

43.421

99.13

0.43