Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   K7X33_RS01340 Genome accession   NZ_CP082355
Coordinates   304122..305618 (+) Length   498 a.a.
NCBI ID   WP_001040617.1    Uniprot ID   -
Organism   Escherichia coli strain VCSW5     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 299122..310618
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K7X33_RS01310 (K7X33_01310) tusA 299369..299614 (+) 246 WP_000130621.1 sulfurtransferase TusA -
  K7X33_RS01315 (K7X33_01315) zntA 299716..301914 (-) 2199 WP_000106575.1 Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase ZntA -
  K7X33_RS01320 (K7X33_01320) yhhN 301988..302614 (-) 627 WP_000964707.1 lysoplasmalogenase -
  K7X33_RS01325 (K7X33_01325) yhhM 302755..303114 (+) 360 WP_000042896.1 DUF2500 domain-containing protein -
  K7X33_RS01330 (K7X33_01330) yhhL 303117..303386 (-) 270 WP_001295207.1 DUF1145 family protein -
  K7X33_RS01335 (K7X33_01335) rsmD 303376..303972 (-) 597 WP_000743193.1 16S rRNA (guanine(966)-N(2))-methyltransferase -
  K7X33_RS01340 (K7X33_01340) pilA 304122..305618 (+) 1497 WP_001040617.1 signal recognition particle-docking protein FtsY Machinery gene
  K7X33_RS01345 (K7X33_01345) ftsE 305621..306289 (+) 669 WP_000617723.1 cell division ATP-binding protein FtsE -
  K7X33_RS01350 (K7X33_01350) ftsX 306282..307340 (+) 1059 WP_001042003.1 permease-like cell division protein FtsX -
  K7X33_RS01355 (K7X33_01355) rpoH 307585..308439 (+) 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
  K7X33_RS01360 (K7X33_01360) livJ 308711..309814 (+) 1104 WP_001350438.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
  K7X33_RS01365 (K7X33_01365) - 309963..310247 (+) 285 WP_000117644.1 DUF1778 domain-containing protein -
  K7X33_RS01370 (K7X33_01370) - 310254..310451 (+) 198 Protein_272 N-acetyltransferase -

Sequence

Protein


Download         Length: 498 a.a.        Molecular weight: 54610.42 Da        Isoelectric Point: 4.1468

>NTDB_id=528325 K7X33_RS01340 WP_001040617.1 304122..305618(+) (pilA) [Escherichia coli strain VCSW5]
MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVPAQEPAKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVAE
SEKAQPEAEVVAQPEPVVEETPEPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
AAEAAEEAVMVVPPAEEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISLFRGKKIDDDLFEELEEQLLIAD
VGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEMGEILAKVDEPLNVEGKAPFVILMVGVNGVGKTTTIGKLARQFEQ
QGKSVMLAAGDTFRAAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKSHLMEELKK
IVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLDGTAKGGVIFSVADQFGIPIRYIGVGERIED
LRPFKADDFIEALFARED

Nucleotide


Download         Length: 1497 bp        

>NTDB_id=528325 K7X33_RS01340 WP_001040617.1 304122..305618(+) (pilA) [Escherichia coli strain VCSW5]
ATGGCGAAAGAAAAAAAACGTGGCTTTTTTTCCTGGCTGGGCTTTGGTCAAAAAGAGCAGACCCCGGAAAAAGAGACAGA
AGTTCAGAATGAACAACCGGTTGTAGAAGAAATCGTTCCGGCGCAAGAGCCTGCGAAGGCTTCTGAACAAGCCGTTGAAG
AGCAGCCGCAGGCGCATACTGAAGCCGAGGCGGAAACTTTTGCTGCCGACGTTGTGGAAGTCACTGAACAGGTTGCTGAA
AGTGAAAAAGCGCAGCCTGAAGCGGAAGTCGTTGCACAGCCGGAACCGGTCGTAGAAGAAACGCCGGAGCCAGTGGCTAT
CGAGCGTGAAGAGCTGCCGTTGCCGGAAGACGTCAACGCCGAAGAGGTTTCGCCAGAAGAGTGGCAGGCCGAAGCGGAAA
CCGTAGAGATTGTCGAAGCGGCGGAAGAAGAAGCGGCTAAAGAAGAAATCACCGATGAAGAGCTGGAAGCACAGGCGCTG
GCTGCCGAAGCAGCAGAAGAGGCGGTGATGGTGGTTCCTCCGGCAGAAGAAGAGCAGCCGGTGGAAGAAATCGCTCAGGA
GCAGGAAAAACCGACCAAAGAAGGTTTCTTCGCGCGCCTGAAACGCAGCCTGTTAAAAACCAAAGAAAATCTCGGTTCCG
GATTTATCAGCCTGTTCCGCGGTAAAAAAATCGACGATGATCTGTTTGAGGAGCTGGAAGAGCAGCTGTTGATCGCCGAT
GTGGGCGTGGAAACCACACGTAAAATTATCACCAATCTGACGGAAGGCGCATCCCGCAAGCAGCTTCGTGATGCCGAGGC
GCTCTATGGCCTGCTGAAAGAAGAGATGGGTGAGATTCTGGCGAAAGTCGATGAGCCGCTGAATGTTGAAGGCAAAGCGC
CGTTTGTGATCCTGATGGTGGGCGTCAACGGTGTGGGTAAAACCACGACGATTGGTAAGCTGGCGCGTCAGTTTGAGCAG
CAGGGTAAATCGGTGATGCTGGCGGCGGGCGATACTTTCCGTGCGGCAGCGGTTGAACAGCTTCAGGTCTGGGGTCAGCG
CAACAATATTCCGGTGATTGCCCAGCATACCGGTGCGGATTCCGCCTCTGTTATCTTCGACGCCATTCAGGCCGCTAAAG
CGCGTAATATCGACGTCCTGATTGCCGATACCGCCGGACGCCTGCAGAACAAATCGCACCTGATGGAAGAGTTGAAGAAA
ATCGTCCGCGTGATGAAGAAACTCGACGTTGAAGCGCCGCATGAAGTTATGCTGACTATCGATGCCAGCACCGGGCAGAA
CGCGGTAAGCCAGGCCAAACTGTTCCATGAAGCCGTTGGCTTAACCGGCATCACGCTAACGAAACTGGACGGCACGGCGA
AAGGCGGGGTAATTTTCTCGGTGGCTGACCAGTTTGGTATCCCTATCCGCTACATTGGTGTCGGCGAACGTATTGAGGAT
TTGCGTCCGTTTAAGGCGGACGACTTTATAGAGGCACTTTTTGCCCGAGAGGATTAA

Domains

Predicted by InterProScan.

SRP54-type protein, GTPase domain (SRP54)

(294-494)

SRP54-type protein, helical bundle domain (SRP54_N)

(211-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

48.831

77.309

0.378