Detailed information NTG0528323

Bioinformatically predicted

Overview

• Name: amiE
• Type: Regulator
• Locus tag: K7X33_RS00885
• Genome accession: NZ_CP082355
• Coordinates: 200631..201614 (+)
• Length: 327 a.a.
• NCBI ID: WP_001196486.1
• Uniprot ID: A7ZT85
• Organism: Escherichia coli strain VCSW5
• Function: internalize XIP (predicted from homology) Competence regulation

Genomic Context

Location: 195631..206614

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
K7X33_RS00870 (K7X33_00870)	dppA	196931..198538 (+)	1608	WP_001222876.1	dipeptide ABC transporter substrate-binding protein DppA	-
K7X33_RS00875 (K7X33_00875)	dppB	198689..199708 (+)	1020	WP_000938855.1	dipeptide ABC transporter permease DppB	-
K7X33_RS00880 (K7X33_00880)	dppC	199718..200620 (+)	903	WP_000084677.1	dipeptide ABC transporter permease DppC	-
K7X33_RS00885 (K7X33_00885)	amiE	200631..201614 (+)	984	WP_001196486.1	dipeptide ABC transporter ATP-binding protein	Regulator
K7X33_RS00890 (K7X33_00890)	dppF	201611..202615 (+)	1005	WP_000107036.1	dipeptide ABC transporter ATP-binding subunit DppF	-
K7X33_RS00895 (K7X33_00895)	yhjV	202645..203916 (-)	1272	WP_001361534.1	aromatic amino acid transport family protein	-
K7X33_RS00900 (K7X33_00900)	-	204392..204499 (+)	108	WP_000170743.1	type I toxin-antitoxin system toxin Ldr family protein	-
K7X33_RS00905 (K7X33_00905)	-	204875..204982 (+)	108	WP_001295224.1	type I toxin-antitoxin system toxin Ldr family protein	-
K7X33_RS00910 (K7X33_00910)	-	205358..205465 (+)	108	WP_000170734.1	type I toxin-antitoxin system toxin Ldr family protein	-

Sequence

Protein

• Length: 327 a.a.
• Molecular weight: 35844.36 Da
• Isoelectric Point: 6.5814

>NTDB_id=528323 K7X33_RS00885 WP_001196486.1 200631..201614(+) (amiE) [Escherichia coli strain VCSW5]
MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGSGKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISE
KERRNLVGAEVAMIFQDPMTSLNPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVAEAAHKIIVMYAGQVVETGDAHA
IFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPGKYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLD
DAGRPTL

Nucleotide

• Length: 984 bp

>NTDB_id=528323 K7X33_RS00885 WP_001196486.1 200631..201614(+) (amiE) [Escherichia coli strain VCSW5]
ATGGCGTTATTAAATGTAGATAAATTATCGGTGCATTTCGGCGACGAAAGCGCACCGTTCCGCGCCGTAGACCGCATCAG
CTACAGCGTAAAACAGGGCGAAGTGGTCGGGATTGTGGGTGAGTCCGGCTCTGGTAAATCGGTCAGTTCGCTGGCGATTA
TGGGGCTGATTGATTATCCGGGCCGCGTAATGGCGGAAAAGCTGGAGTTTAACGGTCAGGATTTACAGCGTATCTCAGAA
AAAGAGCGCCGCAACCTGGTGGGTGCCGAAGTGGCGATGATCTTCCAGGACCCGATGACCAGCCTTAACCCGTGCTACAC
CGTGGGTTTCCAGATTATGGAAGCGATTAAGGTGCATCAGGGCGGCAACAAAAGTACCCGCCGTCAGCGGGCGATTGACC
TGCTGAATCAGGTCGGTATTCCCGATCCGGCTTCGCGTCTGGATGTTTACCCGCATCAGCTTTCCGGCGGCATGAGCCAG
CGCGTGATGATCGCCATGGCGATTGCCTGTCGGCCAAAACTGCTGATTGCCGATGAACCAACCACTGCGCTGGACGTGAC
CATTCAGGCGCAAATTATCGAACTGCTGCTGGAGCTACAGCAGAAAGAGAACATGGCGCTGGTGTTGATCACTCATGACC
TGGCTCTGGTGGCGGAAGCGGCACATAAAATCATCGTGATGTATGCAGGCCAGGTGGTGGAAACGGGCGATGCCCACGCC
ATCTTCCATGCGCCGCGTCATCCATATACTCAGGCATTGCTGCGTGCGCTGCCGGAATTTGCTCAGGACAAAGAACGTCT
GGCGTCGTTGCCAGGTGTTGTTCCCGGCAAGTATGACCGCCCGAACGGCTGCCTGCTTAACCCGCGCTGCCCCTATGCCA
CTGACAGATGTCGCGCTGAAGAACCGGCGCTGAATATGCTCGCTGACGGGCGTCAGTCCAAATGCCATTACCCACTTGAT
GATGCCGGGAGGCCGACACTATGA

Domains

Predicted by InterProScan.

• Oligopeptide/dipeptide transporter, C-terminal region (oligo_HPY) (233-297)

• ABC transporter (ABC_tran) (24-181)

3D structure

• AlphaFold DB: A7ZT85

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
amiE	Streptococcus salivarius strain HSISS4	43.302	98.165	0.425
amiE	Streptococcus thermophilus LMG 18311	42.991	98.165	0.422
amiE	Streptococcus thermophilus LMD-9	42.991	98.165	0.422
oppD	Streptococcus mutans UA159	41.379	97.554	0.404