Detailed information NTG0052828

Bioinformatically predicted

Overview

• Name: rapF
• Type: Regulator
• Locus tag: U712_RS01645
• Genome accession: NC_022898
• Coordinates: 304435..305556 (+)
• Length: 373 a.a.
• NCBI ID: WP_003246478.1
• Uniprot ID: O34327
• Organism: Bacillus subtilis PY79
• Function: inhibit the DNA-binding function of ComA (predicted from homology) Competence regulation

Genomic Context

Location: 299435..310556

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
U712_RS01625 (U712_01435)	ycdA	299443..300507 (-)	1065	WP_003234750.1	DUF4352 domain-containing protein	-
U712_RS21135	-	300530..300661 (-)	132	WP_003246334.1	hypothetical protein	-
U712_RS01630 (U712_01440)	ycdB	300835..302253 (+)	1419	WP_003246352.1	YcdB/YcdC domain-containing protein	-
U712_RS01635 (U712_01445)	ycdC	302440..303801 (+)	1362	WP_010886397.1	YcdB/YcdC domain-containing protein	-
U712_RS01640 (U712_01450)	cwlK	303809..304312 (-)	504	WP_009966473.1	peptidoglycan L-alanyl-D-glutamate endopeptidase CwlK	-
U712_RS01645 (U712_01455)	rapF	304435..305556 (+)	1122	WP_003246478.1	response regulator aspartate phosphatase RapJ	Regulator
U712_RS01650 (U712_01460)	ycdF	305663..306439 (+)	777	WP_003246415.1	SDR family oxidoreductase	-
U712_RS01655 (U712_01465)	ycdG	306464..308149 (+)	1686	WP_003234736.1	alpha-glucosidase	-
U712_RS01660 (U712_01470)	znuA	308337..309296 (+)	960	WP_003234733.1	metal ABC transporter substrate-binding protein	-
U712_RS01665 (U712_01475)	znuC	309352..310047 (+)	696	WP_003234730.1	zinc ABC transporter ATP-binding protein ZnuC	-

Sequence

Protein

• Length: 373 a.a.
• Molecular weight: 44414.31 Da
• Isoelectric Point: 4.8371

>NTDB_id=52828 U712_RS01645 WP_003246478.1 304435..305556(+) (rapF) [Bacillus subtilis PY79]
MRAKIPSEEVAVKLNEWYKLIRAFEADQAEALKQEIEYDLEDMEENQDLLLYFSLMEFRHRIMLDKLMPVKDSDTKPPFS
DMLNEIESNQQKLTGLLEYYFYYFRGMYEFKQKNFILAIDHYKHAEEKLEYVEDEIEKAEFLFKVAEVYYHIKQTYFSMN
YASQALDIYTKYELYGRRRVQCEFIIAGNLTDVYHHEKALTHLCSALEHARQLEEAYMIAAAYYNVGHCKYSLGDYKEAE
GYFKTAAAIFEEHNFQQAVQAVFSLTHIYCKEGKYDKAVEAYDRGIKSAAEWEDDMYLTKFRLIHELYLGSGDLNVLTEC
FDLLESRQLLADAEDLLHDTAERFNQLEHYESAAFFYRRLMNIKKKLAEQRFQ

Nucleotide

• Length: 1122 bp

>NTDB_id=52828 U712_RS01645 WP_003246478.1 304435..305556(+) (rapF) [Bacillus subtilis PY79]
ATGAGAGCAAAGATTCCATCAGAAGAAGTAGCAGTAAAGCTGAATGAATGGTACAAGCTCATTCGCGCATTTGAAGCAGA
TCAAGCAGAAGCGTTAAAGCAGGAGATTGAATATGATTTAGAAGATATGGAAGAGAATCAGGACTTGCTTTTATATTTTT
CTTTGATGGAATTCCGGCATCGAATCATGCTTGATAAGCTGATGCCGGTGAAAGACAGCGATACCAAGCCTCCGTTCTCT
GATATGCTGAACGAAATTGAAAGCAATCAGCAAAAACTCACGGGCTTACTGGAATACTACTTTTATTATTTCAGAGGAAT
GTACGAATTCAAGCAGAAAAATTTCATATTAGCGATTGACCATTATAAACATGCCGAGGAAAAGCTCGAGTATGTCGAGG
ATGAGATCGAAAAAGCTGAGTTTCTTTTTAAAGTCGCAGAAGTGTATTATCACATCAAACAAACGTATTTTTCCATGAAT
TATGCGAGCCAGGCGCTTGATATCTATACGAAATATGAACTGTACGGTCGCCGCCGGGTTCAGTGTGAATTTATCATCGC
CGGAAATTTGACCGATGTGTATCATCATGAAAAAGCACTGACCCACTTGTGCAGCGCTTTAGAACATGCCAGGCAGCTTG
AAGAAGCGTACATGATCGCTGCTGCCTATTATAATGTCGGACACTGTAAGTACAGTCTGGGTGATTACAAAGAAGCGGAG
GGCTATTTCAAAACAGCCGCCGCCATTTTTGAGGAGCACAACTTTCAGCAAGCTGTTCAAGCTGTATTTTCACTGACCCA
TATCTACTGTAAAGAGGGAAAATACGACAAGGCAGTAGAGGCTTATGACCGCGGGATAAAGAGCGCGGCTGAATGGGAGG
ATGACATGTACTTGACGAAATTCCGCCTCATTCATGAGCTGTATCTTGGAAGCGGCGACCTGAACGTGCTTACAGAATGC
TTTGACCTGCTGGAATCCAGGCAGCTTCTTGCTGACGCCGAAGATCTGCTGCATGATACAGCGGAACGTTTCAATCAGTT
GGAACATTACGAGTCCGCCGCTTTCTTTTACCGCAGGCTGATGAATATTAAAAAGAAGCTTGCCGAGCAGCGTTTTCAAT
AG

Domains

Predicted by InterProScan.

• response regulator aspartate phosphatase H, N terminal (RapH_N) (4-65)

• Tetratricopeptide repeat (TPR_12) (196-252)

3D structure

• PDB: 4GYO

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
rapF	Bacillus subtilis subsp. subtilis str. 168	42.391	98.66	0.418
rapC	Bacillus subtilis subsp. subtilis str. 168	41.257	98.123	0.405