Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   K7H97_RS01310 Genome accession   NZ_CP082349
Coordinates   284408..285904 (+) Length   498 a.a.
NCBI ID   WP_001461670.1    Uniprot ID   -
Organism   Escherichia coli strain AL.H8     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 279408..290904
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K7H97_RS01280 (K7H97_01280) tusA 279655..279900 (+) 246 WP_000130621.1 sulfurtransferase TusA -
  K7H97_RS01285 (K7H97_01285) zntA 280002..282200 (-) 2199 WP_000106506.1 Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase ZntA -
  K7H97_RS01290 (K7H97_01290) yhhN 282274..282900 (-) 627 WP_000964718.1 lysoplasmalogenase -
  K7H97_RS01295 (K7H97_01295) yhhM 283041..283400 (+) 360 WP_000042892.1 DUF2500 domain-containing protein -
  K7H97_RS01300 (K7H97_01300) yhhL 283403..283672 (-) 270 WP_001295207.1 DUF1145 family protein -
  K7H97_RS01305 (K7H97_01305) rsmD 283662..284258 (-) 597 WP_000743193.1 16S rRNA (guanine(966)-N(2))-methyltransferase -
  K7H97_RS01310 (K7H97_01310) pilA 284408..285904 (+) 1497 WP_001461670.1 signal recognition particle-docking protein FtsY Machinery gene
  K7H97_RS01315 (K7H97_01315) ftsE 285907..286575 (+) 669 WP_000617723.1 cell division ATP-binding protein FtsE -
  K7H97_RS01320 (K7H97_01320) ftsX 286568..287626 (+) 1059 WP_001042003.1 permease-like cell division protein FtsX -
  K7H97_RS01325 (K7H97_01325) rpoH 287871..288725 (+) 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
  K7H97_RS01330 (K7H97_01330) livJ 288996..290099 (+) 1104 WP_001021989.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
  K7H97_RS01335 (K7H97_01335) panM 290298..290681 (-) 384 WP_000778785.1 aspartate 1-decarboxylase autocleavage activator PanM -

Sequence

Protein


Download         Length: 498 a.a.        Molecular weight: 54729.54 Da        Isoelectric Point: 4.1468

>NTDB_id=528247 K7H97_RS01310 WP_001461670.1 284408..285904(+) (pilA) [Escherichia coli strain AL.H8]
MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEPVKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVAE
SEKAQPEAEVVAQPEPVVEETPEPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
AAEAAEEAVMVVPPTEEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISLFRGKKIDDDLFEELEEQLLIAD
VGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEMGEILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQ
QGKSVMLAAGDTFRAAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKSHLMEELKK
IVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLDGTAKGGVIFSVADQFGIPIRYIGVGERIED
LRPFKADDFIEALFARED

Nucleotide


Download         Length: 1497 bp        

>NTDB_id=528247 K7H97_RS01310 WP_001461670.1 284408..285904(+) (pilA) [Escherichia coli strain AL.H8]
ATGGCGAAAGAAAAAAAACGTGGCTTTTTTTCCTGGCTGGGCTTTGGTCAAAAAGAGCAGACCCCGGAAAAAGAGACAGA
AGTTCAGAATGAACAACCGGTTGTAGAAGAAATCGTTCAGGCGCAAGAGCCTGTGAAGGCTTCAGAACAAGCCGTTGAAG
AGCAGCCGCAGGCGCATACTGAGGCCGAGGCGGAAACTTTTGCTGCCGACGTTGTGGAAGTCACTGAACAGGTTGCTGAA
AGTGAAAAAGCGCAGCCTGAAGCGGAAGTCGTTGCACAGCCGGAACCGGTCGTAGAAGAAACGCCGGAGCCAGTGGCTAT
CGAACGTGAAGAGCTGCCGTTGCCGGAAGACGTCAACGCCGAAGAGGTTTCGCCAGAAGAGTGGCAGGCTGAAGCGGAAA
CCGTAGAGATTGTCGAAGCGGCGGAAGAAGAAGCGGCTAAAGAAGAAATCACCGACGAAGAGCTGGAAGCACAGGCGCTG
GCTGCCGAAGCGGCAGAAGAGGCGGTGATGGTGGTTCCTCCGACAGAAGAAGAGCAGCCGGTGGAAGAAATCGCTCAGGA
GCAGGAAAAACCGACCAAAGAAGGTTTCTTCGCGCGCCTGAAACGCAGCCTGTTAAAAACCAAAGAAAATCTCGGTTCCG
GATTTATCAGCCTGTTCCGCGGTAAAAAAATCGACGATGATCTGTTTGAAGAGCTGGAAGAACAGTTGTTGATCGCCGAT
GTGGGCGTGGAAACCACACGTAAAATTATCACCAATCTGACGGAAGGCGCATCCCGCAAGCAGCTTCGTGATGCCGAGGC
GCTCTATGGCCTGCTGAAAGAAGAGATGGGCGAGATTCTGGCGAAAGTCGATGAGCCGCTGAATGTTGAAGGTAAAACGC
CGTTCGTGATCCTGATGGTGGGCGTCAACGGTGTGGGTAAAACCACGACGATTGGTAAGCTGGCGCGTCAGTTTGAGCAG
CAGGGTAAATCGGTGATGCTGGCGGCGGGCGATACTTTCCGTGCAGCTGCGGTTGAACAGCTTCAGGTCTGGGGTCAGCG
CAACAATATTCCGGTGATTGCCCAGCATACCGGGGCGGATTCCGCCTCTGTTATCTTCGACGCTATTCAGGCAGCTAAAG
CGCGTAATATCGACGTCCTGATTGCCGATACAGCCGGACGCCTGCAGAACAAATCGCACCTGATGGAAGAGTTGAAGAAA
ATCGTCCGCGTGATGAAGAAACTCGACGTTGAAGCGCCGCATGAAGTTATGCTGACTATTGATGCCAGCACCGGGCAGAA
CGCGGTAAGCCAGGCCAAACTGTTCCACGAAGCTGTTGGCTTAACCGGCATCACGCTAACGAAACTGGACGGCACGGCGA
AAGGCGGGGTAATTTTCTCGGTGGCTGACCAGTTTGGTATCCCTATCCGCTACATTGGTGTCGGCGAACGTATTGAGGAT
TTGCGTCCGTTTAAGGCGGACGACTTTATAGAGGCACTTTTTGCCCGAGAGGATTAA

Domains

Predicted by InterProScan.

SRP54-type protein, GTPase domain (SRP54)

(294-494)

SRP54-type protein, helical bundle domain (SRP54_N)

(211-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

48.831

77.309

0.378