Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   K6983_RS18750 Genome accession   NZ_CP082327
Coordinates   3840672..3841112 (+) Length   146 a.a.
NCBI ID   WP_000360904.1    Uniprot ID   P36647
Organism   Escherichia coli strain B2     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3835672..3846112
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K6983_RS18730 (K6983_18660) aroP 3836670..3838043 (+) 1374 WP_000969915.1 aromatic amino acid transporter AroP -
  K6983_RS18735 (K6983_18665) ampE 3838086..3838940 (-) 855 WP_000172005.1 beta-lactamase regulator AmpE -
  K6983_RS18740 (K6983_18670) ampD 3838937..3839488 (-) 552 WP_000923721.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  K6983_RS18745 (K6983_18675) nadC 3839576..3840469 (+) 894 WP_001135174.1 carboxylating nicotinate-nucleotide diphosphorylase -
  K6983_RS18750 (K6983_18680) pilA 3840672..3841112 (+) 441 WP_000360904.1 prepilin peptidase-dependent pilin Machinery gene
  K6983_RS18755 (K6983_18685) pilB 3841122..3842507 (+) 1386 WP_001025145.1 type II secretion system protein GspE Machinery gene
  K6983_RS18760 (K6983_18690) hofC 3842497..3843699 (+) 1203 WP_000157266.1 protein transport protein HofC -
  K6983_RS18765 (K6983_18695) guaC 3843734..3844777 (-) 1044 WP_001217338.1 GMP reductase -
  K6983_RS18770 (K6983_18700) - 3844933..3844977 (-) 45 WP_120795372.1 protein YacM -
  K6983_RS18775 (K6983_18705) coaE 3845002..3845622 (+) 621 WP_001269520.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15621.75 Da        Isoelectric Point: 4.3938

>NTDB_id=528220 K6983_RS18750 WP_000360904.1 3840672..3841112(+) (pilA) [Escherichia coli strain B2]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=528220 K6983_RS18750 WP_000360904.1 3840672..3841112(+) (pilA) [Escherichia coli strain B2]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGGCTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P36647

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363