NTDB

Overview

Name	dprA	Type	Machinery gene
Locus tag	K6983_RS02160	Genome accession	NZ_CP082327
Coordinates	446982..448106 (+)	Length	374 a.a.
NCBI ID	WP_000228551.1	Uniprot ID	P30852
Organism	Escherichia coli strain B2
Function	ssDNA binding; loading RecA onto ssDNA (predicted from homology) DNA processing

Genomic Context

Location: 441982..453106

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
K6983_RS02135 (K6983_02130)	mscL	442108..442518 (-)	411	WP_000022442.1	large-conductance mechanosensitive channel protein MscL	-
K6983_RS02140 (K6983_02135)	trkA	442648..444024 (-)	1377	WP_000691382.1	Trk system potassium transporter TrkA	-
K6983_RS02145 (K6983_02140)	rsmB	444046..445335 (-)	1290	WP_000744778.1	16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB	-
K6983_RS02150 (K6983_02145)	fmt	445381..446328 (-)	948	WP_000004473.1	methionyl-tRNA formyltransferase	-
K6983_RS02155 (K6983_02150)	def	446343..446852 (-)	510	WP_000114984.1	peptide deformylase	-
K6983_RS02160 (K6983_02155)	dprA	446982..448106 (+)	1125	WP_000228551.1	DNA-protecting protein DprA	Machinery gene
K6983_RS02165 (K6983_02160)	smg	448078..448551 (+)	474	WP_000460680.1	DUF494 family protein Smg	-
K6983_RS02170 (K6983_02165)	yrdD	448580..449122 (+)	543	WP_001129722.1	type I DNA topoisomerase	-
K6983_RS02175 (K6983_02170)	tsaC	449127..449699 (+)	573	WP_001301412.1	L-threonylcarbamoyladenylate synthase type 1 TsaC	-
K6983_RS02180 (K6983_02175)	aroE	449704..450522 (+)	819	WP_000451243.1	shikimate dehydrogenase	-
K6983_RS02185 (K6983_02180)	yrdB	450519..450776 (+)	258	Protein_435	DUF1488 domain-containing protein	-
K6983_RS02190 (K6983_02185)	yrdA	450752..451306 (-)	555	WP_001286216.1	gamma carbonic anhydrase family protein	-

Sequence

Protein

Download Length: 374 a.a. Molecular weight: 40954.92 Da Isoelectric Point: 6.3122

                                >NTDB_id=528174 K6983_RS02160 WP_000228551.1 446982..448106(+) (dprA) [Escherichia coli strain B2]

MVDTEIWLRLMSISSLYGDDMVRIAHWVAKQSHIDAVVLQQTGLTLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSE

FYPPQLLATTDYPGALFVEGELHALHSFQLAVVGSRAHSWYGERWGRLFCETLATRGVTITSGLARGIDGVAHKAALQVN

GVSIAVLGNGLNTIHPRRHARLAASLLEQGGALVSEFPLDVPPLAYNFPRRNRIISGLSKGVLVVEAALRSGSLVTARCA

LEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEPEEILENLQFGLHWLPDAPENSFYSPDQQDVALPFPELLANVGDE

VTPVDVVAERAGQPVPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV

Nucleotide

Download Length: 1125 bp

                                >NTDB_id=528174 K6983_RS02160 WP_000228551.1 446982..448106(+) (dprA) [Escherichia coli strain B2]

ATGGTCGATACAGAAATTTGGCTGCGTTTAATGAGTATCAGCAGCTTGTACGGCGATGATATGGTCCGTATCGCTCACTG

GGTGGCAAAACAGTCGCATATTGATGCGGTTGTATTGCAGCAAACAGGGCTTACATTGCGGCAGGCACAACGCTTTCTTT

CATTTCCACGAAAGAGTATCGAAAGCTCACTTTGTTGGTTGGAGCAACCCAACCATCATTTAATTCCTGCGGACAGCGAA

TTTTATCCTCCTCAACTTCTGGCGACGACAGATTACCCCGGCGCACTGTTTGTTGAAGGAGAACTGCACGCGCTGCATTC

ATTTCAGCTTGCCGTAGTGGGGAGTCGGGCGCATTCATGGTATGGCGAGCGATGGGGACGATTATTTTGCGAAACTCTGG

CGACGCGTGGAGTGACAATTACGAGTGGACTGGCGCGTGGAATCGATGGTGTAGCGCATAAAGCAGCCTTACAGGTAAAT

GGCGTCAGCATTGCTGTATTGGGGAATGGACTTAATACCATTCATCCCCGCCGTCATGCCCGACTGGCTGCCAGTCTGCT

TGAACAGGGGGGCGCTCTCGTCTCGGAATTTCCCCTCGATGTTCCACCCCTTGCTTACAATTTCCCACGAAGAAATCGCA

TTATCAGTGGTCTAAGTAAAGGTGTACTGGTGGTGGAAGCGGCTTTGCGTAGTGGTTCGCTGGTGACAGCACGTTGTGCG

CTTGAGCAGGGGCGAGAAGTTTTTGCCTTGCCAGGTCCAATAGGGAATCCGGGAAGCGAAGGGCCTCACTGGTTAATAAA

ACAAGGTGCGATTCTTGTGACGGAACCGGAAGAAATTCTGGAAAACTTGCAATTTGGATTGCACTGGTTGCCAGACGCCC

CTGAAAATTCATTTTATTCACCAGATCAGCAAGACGTGGCATTGCCATTTCCTGAGCTCCTGGCTAACGTAGGAGATGAG

GTAACACCTGTTGACGTCGTCGCTGAGCGTGCCGGCCAACCTGTGCCAGAGGTAGTTACTCAACTACTCGAACTGGAGTT

AGCAGGATGGATCGCAGCTGTACCCGGCGGCTATGTCCGATTGAGGAGGGCATGCCATGTTCGACGTACTAATGTATTTG

TTTGA

Domains

Predicted by InterProScan.

DNA recombination-mediator protein A (DNA_processg_A)

(67-274)

DprA winged helix domain (WHD_DprA)

(312-357)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure
AlphaFold DB	P30852

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
dprA	Vibrio campbellii strain DS40M4	48.238	98.663	0.476
dprA	Vibrio cholerae O1 biovar El Tor strain E7946	49.355	82.888	0.409
dprA	Vibrio cholerae strain A1552	49.355	82.888	0.409
dprA	Haemophilus influenzae Rd KW20	45.104	90.107	0.406
dprA	Glaesserella parasuis strain SC1401	44.807	90.107	0.404
dprA	Legionella pneumophila strain ERS1305867	44.242	88.235	0.39

Detailed information NTG0528174