Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   I6I49_RS04770 Genome accession   NZ_CP068206
Coordinates   578304..579104 (-) Length   266 a.a.
NCBI ID   WP_004697674.1    Uniprot ID   -
Organism   Acinetobacter johnsonii strain FDAARGOS_1092     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 573304..584104
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I6I49_RS04750 (I6I49_04750) - 574385..575083 (-) 699 WP_184118597.1 tetratricopeptide repeat protein -
  I6I49_RS04755 (I6I49_04755) hisS 575111..576406 (-) 1296 WP_005401705.1 histidine--tRNA ligase -
  I6I49_RS04760 (I6I49_04760) ispG 576403..577518 (-) 1116 WP_004697677.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  I6I49_RS04765 (I6I49_04765) - 577537..578313 (-) 777 WP_004697676.1 helix-turn-helix domain-containing protein -
  I6I49_RS04770 (I6I49_04770) pilF 578304..579104 (-) 801 WP_004697674.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  I6I49_RS04775 (I6I49_04775) rlmN 579121..580356 (-) 1236 WP_004697671.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  I6I49_RS04780 (I6I49_04780) ndk 580479..580910 (-) 432 WP_004697669.1 nucleoside-diphosphate kinase -
  I6I49_RS04785 (I6I49_04785) iscX 581021..581221 (-) 201 WP_004697668.1 Fe-S cluster assembly protein IscX -
  I6I49_RS04790 (I6I49_04790) - 581312..581863 (-) 552 WP_004697666.1 phosphatase PAP2 family protein -
  I6I49_RS04795 (I6I49_04795) - 582056..583336 (-) 1281 WP_201952505.1 glycosyltransferase family 1 protein -
  I6I49_RS04800 (I6I49_04800) - 583418..583888 (-) 471 WP_005399975.1 IS5/IS1182 family transposase -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 30076.05 Da        Isoelectric Point: 9.1263

>NTDB_id=527963 I6I49_RS04770 WP_004697674.1 578304..579104(-) (pilF) [Acinetobacter johnsonii strain FDAARGOS_1092]
MKKPVSKLYLALTLGVSVWYLVGCQTVNTKGDPSKAVQVRTQLAAEYIKSNDYDAAKRTLDQALDIDSRDASANMMMGVL
LQREGSPQNVEKAERYFKHAIAAEPKNAQARNNYGTYLYQIGRYNDAIDQLQVAGSTLGYDQRYRALENLGRAYLQVGRV
AEAEAAFKQALQVNSGAYLAMIEMAEISYLRQQNAEATQYYEQFVRAVGEKNLDARALWIGIRIARANHDTMGSQVLVNQ
LRALYPDSQEYKRYLQLQYTTEAVWK

Nucleotide


Download         Length: 801 bp        

>NTDB_id=527963 I6I49_RS04770 WP_004697674.1 578304..579104(-) (pilF) [Acinetobacter johnsonii strain FDAARGOS_1092]
TTGAAAAAACCCGTTTCTAAACTTTACCTTGCGCTGACACTGGGTGTGTCAGTGTGGTACTTGGTGGGTTGCCAAACCGT
GAATACGAAAGGTGACCCAAGTAAAGCAGTACAAGTTAGAACGCAATTGGCGGCTGAATATATTAAGTCGAATGATTACG
ATGCTGCGAAACGCACATTGGATCAAGCATTAGACATCGACTCTCGTGATGCATCTGCAAATATGATGATGGGCGTTTTG
TTACAGCGTGAAGGCAGTCCACAAAATGTGGAAAAGGCAGAGCGTTATTTTAAGCATGCAATTGCGGCTGAGCCAAAAAA
TGCACAGGCTCGTAACAACTACGGTACGTATTTATATCAAATTGGGCGTTATAATGACGCGATTGATCAACTTCAAGTTG
CAGGTTCAACTTTAGGGTATGATCAACGTTATCGAGCATTGGAGAATTTAGGTCGTGCGTATCTACAAGTAGGTCGAGTG
GCGGAAGCAGAAGCTGCTTTTAAACAAGCTCTACAGGTAAACTCAGGTGCCTATCTAGCCATGATCGAAATGGCGGAAAT
TTCATATTTACGTCAGCAAAATGCTGAAGCTACGCAGTACTATGAACAGTTTGTGCGAGCGGTGGGCGAGAAAAACTTAG
ATGCGCGTGCGCTTTGGATTGGGATCCGAATTGCGCGAGCAAATCATGACACGATGGGTTCGCAAGTACTGGTCAACCAG
TTACGTGCATTGTATCCAGATAGTCAAGAATACAAACGTTATTTGCAATTACAGTACACTACTGAGGCCGTATGGAAGTA
A

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(39-68)

Tetratricopeptide repeat (TPR_16)

(149-206)

Tetratricopeptide repeat (TPR_19)

(89-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

68.657

100

0.692
  pilF Acinetobacter baylyi ADP1

64.662

100

0.647