Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   I6I50_RS02770 Genome accession   NZ_CP068195
Coordinates   561604..562404 (-) Length   266 a.a.
NCBI ID   WP_087014670.1    Uniprot ID   A0A1R7QGX9
Organism   Acinetobacter johnsonii strain FDAARGOS_1093     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 556604..567404
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I6I50_RS02750 (I6I50_02750) - 557685..558383 (-) 699 WP_005401704.1 tetratricopeptide repeat protein -
  I6I50_RS02755 (I6I50_02755) hisS 558411..559706 (-) 1296 WP_062031308.1 histidine--tRNA ligase -
  I6I50_RS02760 (I6I50_02760) ispG 559703..560818 (-) 1116 WP_130235007.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  I6I50_RS02765 (I6I50_02765) - 560837..561613 (-) 777 WP_010327531.1 helix-turn-helix domain-containing protein -
  I6I50_RS02770 (I6I50_02770) pilF 561604..562404 (-) 801 WP_087014670.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  I6I50_RS02775 (I6I50_02775) rlmN 562421..563656 (-) 1236 WP_004697671.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  I6I50_RS02780 (I6I50_02780) ndk 563779..564210 (-) 432 WP_004983508.1 nucleoside-diphosphate kinase -
  I6I50_RS02785 (I6I50_02785) iscX 564321..564521 (-) 201 WP_004697668.1 Fe-S cluster assembly protein IscX -
  I6I50_RS02790 (I6I50_02790) - 564612..565163 (-) 552 WP_125280205.1 phosphatase PAP2 family protein -
  I6I50_RS02795 (I6I50_02795) - 565356..566636 (-) 1281 WP_201706777.1 glycosyltransferase family 1 protein -
  I6I50_RS02800 (I6I50_02800) - 566777..567193 (-) 417 WP_004983505.1 hypothetical protein -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 30090.08 Da        Isoelectric Point: 9.1263

>NTDB_id=527922 I6I50_RS02770 WP_087014670.1 561604..562404(-) (pilF) [Acinetobacter johnsonii strain FDAARGOS_1093]
MKKPVSKLYLALTLGVTVWYLVGCQTVNTKGDPSKAVQVRTQLAAEYIKSNDYDAAKRTLDQALDIDSRDASANMMMGVL
LQREGSPQNVEKAERYFKHAIAAEPKNAQARNNYGTYLYQIGRYNDAIDQLQVAGSTLGYDQRYRALENLGRAYLQVGRV
AEAEAAFKQALQVNSGAYLAMIEMAEISYLRQQNAEATQYYEQFVRAVGEKNLDARALWIGIRIARANHDTMGSQVLVNQ
LRALYPDSQEYKRYLQLQYTTEAVWK

Nucleotide


Download         Length: 801 bp        

>NTDB_id=527922 I6I50_RS02770 WP_087014670.1 561604..562404(-) (pilF) [Acinetobacter johnsonii strain FDAARGOS_1093]
TTGAAAAAACCCGTTTCTAAACTTTACCTTGCGCTGACACTGGGTGTGACAGTGTGGTACTTGGTGGGTTGCCAAACCGT
GAATACGAAAGGTGATCCAAGTAAAGCAGTACAAGTTAGAACGCAGTTGGCGGCTGAATATATTAAGTCGAATGATTACG
ATGCTGCGAAACGTACATTGGATCAGGCATTAGACATCGACTCTCGTGATGCATCTGCAAATATGATGATGGGGGTGTTG
TTACAGCGTGAAGGCAGTCCACAAAATGTGGAAAAGGCAGAGCGTTATTTTAAGCATGCAATTGCGGCTGAGCCAAAAAA
TGCACAGGCTCGTAACAACTACGGTACGTATTTATATCAAATTGGGCGTTATAATGACGCGATTGATCAACTTCAAGTTG
CAGGTTCAACTTTAGGGTATGATCAACGTTATCGAGCATTGGAAAATTTAGGTCGTGCGTATCTACAAGTAGGTCGAGTG
GCGGAAGCAGAAGCTGCTTTTAAACAAGCCCTACAGGTAAACTCAGGTGCCTATCTTGCCATGATCGAAATGGCGGAAAT
TTCATATTTACGTCAGCAAAATGCTGAAGCTACGCAGTACTATGAACAGTTTGTGCGAGCGGTTGGCGAGAAAAACTTAG
ATGCACGTGCGCTTTGGATTGGGATCCGAATTGCGCGAGCAAATCATGACACGATGGGTTCGCAAGTACTGGTCAACCAG
CTACGTGCATTGTATCCAGACAGTCAAGAATACAAACGTTATTTGCAATTACAGTACACTACTGAGGCCGTATGGAAGTA
A

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_16)

(149-206)

Tetratricopeptide repeat (TPR_8)

(39-68)

Tetratricopeptide repeat (TPR_19)

(89-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1R7QGX9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

68.657

100

0.692
  pilF Acinetobacter baylyi ADP1

64.662

100

0.647