Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   I6I53_RS07950 Genome accession   NZ_CP068176
Coordinates   1545748..1546500 (+) Length   250 a.a.
NCBI ID   WP_004994852.1    Uniprot ID   A0A3G9FKH4
Organism   Acinetobacter ursingii strain FDAARGOS_1096     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1540748..1551500
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I6I53_RS07925 (I6I53_07930) - 1541626..1542903 (+) 1278 WP_004994863.1 glycosyltransferase family 1 protein -
  I6I53_RS07930 (I6I53_07935) - 1542933..1543484 (+) 552 WP_004992665.1 phosphatase PAP2 family protein -
  I6I53_RS07935 (I6I53_07940) iscX 1543589..1543789 (+) 201 WP_004994862.1 Fe-S cluster assembly protein IscX -
  I6I53_RS07940 (I6I53_07945) ndk 1543901..1544332 (+) 432 WP_004994861.1 nucleoside-diphosphate kinase -
  I6I53_RS07945 (I6I53_07950) rlmN 1544454..1545680 (+) 1227 WP_004994854.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  I6I53_RS07950 (I6I53_07955) pilF 1545748..1546500 (+) 753 WP_004994852.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  I6I53_RS07955 (I6I53_07960) - 1546491..1547273 (+) 783 WP_004994849.1 helix-turn-helix domain-containing protein -
  I6I53_RS07960 (I6I53_07965) ispG 1547298..1548416 (+) 1119 WP_004992682.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  I6I53_RS07965 (I6I53_07970) hisS 1548420..1549712 (+) 1293 WP_004994847.1 histidine--tRNA ligase -
  I6I53_RS07970 (I6I53_07975) - 1549742..1550443 (+) 702 WP_004994846.1 tetratricopeptide repeat protein -

Sequence

Protein


Download         Length: 250 a.a.        Molecular weight: 28394.23 Da        Isoelectric Point: 9.3924

>NTDB_id=527832 I6I53_RS07950 WP_004994852.1 1545748..1546500(+) (pilF) [Acinetobacter ursingii strain FDAARGOS_1096]
MALLLTGCQSQKTTKDPKKAVQVRTQLAAEYIKSGDLDAAKRALDQALEVNSRDSMANMMMGVLLQQEGSATNLEKADTY
FKRAISEEPTNAQARNNYGTYLYQLKRYNDAIEQFTIAGATLGYDQRYRALENLGRIYVLLGDRVNAEKSFKQALQANRD
SYVSMLELSEIFYLQQQMPAATQMYEQYVRTVGQTNQGARALWVGIRVARANGDRMGMQALINQLRALFPDSSEYKRYLQ
LQYSTEAVWK

Nucleotide


Download         Length: 753 bp        

>NTDB_id=527832 I6I53_RS07950 WP_004994852.1 1545748..1546500(+) (pilF) [Acinetobacter ursingii strain FDAARGOS_1096]
ATGGCGTTGCTGTTAACGGGATGCCAATCTCAAAAAACAACCAAAGACCCTAAAAAAGCGGTGCAGGTTCGTACACAACT
TGCAGCTGAATATATCAAGTCTGGTGATCTGGATGCTGCTAAACGCGCATTGGATCAGGCTTTGGAGGTGAACTCGCGTG
ACTCCATGGCCAATATGATGATGGGTGTACTCTTACAACAAGAAGGTAGTGCAACAAATCTCGAGAAAGCAGACACGTAT
TTTAAACGTGCAATCTCGGAAGAGCCTACCAATGCACAAGCTCGTAATAATTATGGTACGTACTTATACCAATTGAAACG
TTATAATGACGCGATTGAACAATTTACGATTGCAGGGGCAACACTTGGGTATGATCAGCGTTATCGTGCCCTTGAAAATC
TGGGGCGTATCTACGTATTGCTTGGCGATCGTGTGAATGCTGAAAAGTCATTCAAACAAGCTTTACAGGCAAACCGCGAC
TCTTATGTATCTATGTTAGAGTTGTCAGAGATTTTTTATTTGCAACAGCAAATGCCAGCGGCGACTCAAATGTATGAACA
ATATGTGCGTACAGTGGGGCAAACCAATCAGGGGGCGCGTGCACTTTGGGTGGGGATTCGTGTTGCGCGAGCCAATGGTG
ATCGAATGGGAATGCAGGCACTGATCAATCAACTGCGTGCTTTATTTCCCGATAGTTCAGAATATAAACGTTATTTGCAA
TTACAGTATAGTACTGAGGCCGTATGGAAATAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_17)

(81-113)

Tetratricopeptide repeat (TPR_7)

(130-160)

Tetratricopeptide repeat (TPR_8)

(23-52)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3G9FKH4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

81.275

100

0.816
  pilF Acinetobacter baylyi ADP1

77.108

99.6

0.768