Detailed information NTG0527722

Bioinformatically predicted

Overview

• Name: pilA
• Type: Machinery gene
• Locus tag: K7B13_RS07710
• Genome accession: NZ_CP082278
• Coordinates: 1525999..1526988 (+)
• Length: 329 a.a.
• NCBI ID: WP_013352235.1
• Uniprot ID: A0A9P1JH46
• Organism: Bacillus amyloliquefaciens strain 35M
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1520999..1531988

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
K7B13_RS07695 (K7B13_07695)	acpP	1521197..1521430 (+)	234	WP_003154310.1	acyl carrier protein	-
K7B13_RS07700 (K7B13_07700)	rncS	1521569..1522318 (+)	750	WP_013352233.1	ribonuclease III	-
K7B13_RS07705 (K7B13_07705)	smc	1522419..1525979 (+)	3561	WP_212101338.1	chromosome segregation protein SMC	-
K7B13_RS07710 (K7B13_07710)	pilA	1525999..1526988 (+)	990	WP_013352235.1	signal recognition particle-docking protein FtsY	Machinery gene
K7B13_RS07715 (K7B13_07715)	-	1527178..1527510 (+)	333	WP_003154299.1	putative DNA-binding protein	-
K7B13_RS07720 (K7B13_07720)	ffh	1527525..1528865 (+)	1341	WP_212101340.1	signal recognition particle protein	-
K7B13_RS07725 (K7B13_07725)	rpsP	1528971..1529243 (+)	273	WP_003154296.1	30S ribosomal protein S16	-
K7B13_RS07730 (K7B13_07730)	-	1529243..1529488 (+)	246	WP_007611392.1	KH domain-containing protein	-
K7B13_RS07735 (K7B13_07735)	-	1529577..1529963 (+)	387	WP_013352237.1	YlqD family protein	-
K7B13_RS07740 (K7B13_07740)	rimM	1529968..1530492 (+)	525	WP_212101342.1	ribosome maturation factor RimM	-
K7B13_RS07745 (K7B13_07745)	trmD	1530489..1531220 (+)	732	WP_212101344.1	tRNA (guanosine(37)-N1)-methyltransferase TrmD	-
K7B13_RS07750 (K7B13_07750)	rplS	1531361..1531708 (+)	348	WP_003154288.1	50S ribosomal protein L19	-

Sequence

Protein

• Length: 329 a.a.
• Molecular weight: 36152.35 Da
• Isoelectric Point: 5.0201

>NTDB_id=527722 K7B13_RS07710 WP_013352235.1 1525999..1526988(+) (pilA) [Bacillus amyloliquefaciens strain 35M]
MSFFKKLKEKITKQTDSVSEKFKDGLEKTRNSFSNRVNELVSRYRKVDEDFFEELEEVLIGADVGFTTVMELIDELKKEV
KLKNIQDPQEVQSVISEKLVEIYNSGNEEISELNIQDGRLNVILLVGVNGVGKTTTIGKLAHKLKNEGKSVVLAAGDTFR
AGAIEQLEVWGERTGVPVIKQAAGSDPAAVIYDAVHAAKARGADVLICDTAGRLQNKVNLMKELEKVKRVIEREVPEAPH
EVLLALDATTGQNAMAQAKEFSKATNVTGIALTKLDGTAKGGIVLAIRNELHIPVKLVGLGEKVDDLQRFDAESYVYGLF
SDLVEKAEE

Nucleotide

• Length: 990 bp

>NTDB_id=527722 K7B13_RS07710 WP_013352235.1 1525999..1526988(+) (pilA) [Bacillus amyloliquefaciens strain 35M]
ATGAGCTTTTTTAAAAAATTAAAAGAGAAAATCACGAAACAGACAGACTCCGTCTCTGAAAAATTTAAAGATGGCCTTGA
AAAAACAAGAAATTCCTTCTCAAATAGAGTGAACGAACTCGTCTCCCGCTACCGAAAAGTCGACGAAGATTTCTTCGAAG
AGCTTGAGGAAGTGCTGATTGGCGCTGACGTCGGTTTTACAACGGTCATGGAGCTGATTGACGAGCTCAAAAAAGAAGTG
AAGCTGAAAAACATCCAGGACCCTCAGGAAGTGCAGTCCGTCATTTCTGAAAAGCTCGTCGAAATTTATAACAGCGGCAA
CGAGGAGATTTCTGAATTGAATATTCAGGACGGGCGCTTAAACGTTATTCTGCTTGTCGGCGTAAACGGTGTCGGAAAAA
CGACAACGATCGGAAAACTTGCCCATAAGCTGAAGAATGAAGGCAAATCCGTTGTGCTCGCTGCCGGAGACACCTTCAGA
GCCGGAGCGATTGAACAGCTTGAAGTGTGGGGAGAGCGTACAGGCGTGCCTGTCATTAAGCAGGCTGCCGGAAGCGACCC
TGCGGCGGTTATTTACGATGCCGTACACGCGGCTAAAGCAAGAGGCGCGGATGTGCTGATTTGTGATACGGCCGGCCGCC
TTCAGAATAAAGTAAACTTAATGAAAGAGCTTGAAAAGGTCAAACGCGTCATCGAACGTGAAGTCCCTGAGGCTCCGCAT
GAGGTTCTGCTCGCTCTCGACGCGACGACAGGCCAGAACGCGATGGCGCAGGCGAAAGAATTCTCCAAAGCGACGAATGT
GACGGGTATCGCGTTAACGAAGCTTGACGGCACCGCAAAAGGCGGAATTGTTTTGGCGATCCGCAATGAGCTTCATATTC
CCGTAAAACTGGTCGGCCTCGGAGAAAAGGTTGACGATCTGCAGCGGTTTGATGCAGAATCTTACGTATACGGTCTCTTT
TCGGATTTAGTGGAAAAAGCCGAAGAATAG

Domains

Predicted by InterProScan.

• SRP54-type protein, helical bundle domain (SRP54_N) (33-99)

• SRP54-type protein, GTPase domain (SRP54) (121-320)

3D structure

• AlphaFold DB: A0A9P1JH46

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilA	Neisseria gonorrhoeae MS11	45	91.185	0.41