Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   PCC21_RS02415 Genome accession   NC_018525
Coordinates   532522..533904 (-) Length   460 a.a.
NCBI ID   WP_014914021.1    Uniprot ID   A0A3S0ZXC5
Organism   Pectobacterium carotovorum subsp. carotovorum PCC21     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 527522..538904
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PCC21_RS02390 (PCC21_004750) pepP 527716..529041 (-) 1326 WP_014914017.1 Xaa-Pro aminopeptidase -
  PCC21_RS02395 (PCC21_004760) - 529099..529686 (-) 588 WP_014914018.1 YecA family protein -
  PCC21_RS02400 (PCC21_004770) zapA 529879..530208 (+) 330 WP_010284667.1 cell division protein ZapA -
  PCC21_RS02405 (PCC21_004780) - 530506..531153 (+) 648 WP_014914019.1 5-formyltetrahydrofolate cyclo-ligase -
  PCC21_RS02410 (PCC21_004790) nadR 531150..532403 (-) 1254 WP_014914020.1 multifunctional transcriptional regulator/nicotinamide-nucleotide adenylyltransferase/ribosylnicotinamide kinase NadR -
  PCC21_RS02415 (PCC21_004800) radA 532522..533904 (-) 1383 WP_014914021.1 DNA repair protein RadA Machinery gene
  PCC21_RS02420 (PCC21_004810) serB 533922..534899 (-) 978 WP_014914022.1 phosphoserine phosphatase -
  PCC21_RS02425 (PCC21_004820) - 535056..535754 (+) 699 WP_014914023.1 YtjB family periplasmic protein -
  PCC21_RS02430 (PCC21_004830) - 535803..536720 (-) 918 WP_014914024.1 hypothetical protein -
  PCC21_RS02435 (PCC21_004840) prfC 537012..538601 (+) 1590 WP_014914025.1 peptide chain release factor 3 -

Sequence

Protein


Download         Length: 460 a.a.        Molecular weight: 49620.19 Da        Isoelectric Point: 7.4218

>NTDB_id=52702 PCC21_RS02415 WP_014914021.1 532522..533904(-) (radA) [Pectobacterium carotovorum subsp. carotovorum PCC21]
MAKAVKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLASASTSRSDRLTGYAGESAGVSRVQKLSEISLEALPRFSTG
FQEFDRVLGGGVVPGSAILIGGNPGAGKSTLLLQTLCKLSENMKTLYVTGEESLQQVAMRAHRLNLPTQNLNMLSETSIE
QICLIAEQEQPKLMVIDSIQVMHLADIQSSPGSVAQVRETAAYLTRFAKTRGVAIVMVGHVTKDGSLAGPKVLEHCIDCS
VLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQGLREISNPSAIFLSRGDEITSGSSVMVVWEGTRPLLVEIQALVDQ
SMMANPRRVAVGLEQNRLAILLAVLHRHGGLQMSDQDVFVNVVGGVKVTETSADLALLLSLVSSFRDRPLPQDLVIFGEV
GLAGEIRPVPSGQERITEAAKHGFKRAIVPHANMPKKVPASMQVFGVKKLADALAILDDL

Nucleotide


Download         Length: 1383 bp        

>NTDB_id=52702 PCC21_RS02415 WP_014914021.1 532522..533904(-) (radA) [Pectobacterium carotovorum subsp. carotovorum PCC21]
GTGGCAAAAGCCGTCAAACGGGCGTTTGTATGTAATGAATGCGGGGCTGACTACCCGCGCTGGCAAGGGCAGTGCAGCGC
CTGCCATGCCTGGAACACCATTACCGAAGTCCGTCTGGCCTCGGCGTCAACATCACGTTCCGACCGTCTCACCGGCTATG
CGGGCGAAAGCGCTGGCGTCAGCCGGGTACAAAAGCTCTCGGAAATCAGTCTTGAAGCCCTGCCGCGCTTTTCCACCGGT
TTTCAGGAGTTTGACCGCGTTCTGGGCGGCGGCGTCGTTCCGGGCAGCGCGATTCTGATCGGCGGTAACCCTGGCGCGGG
TAAAAGTACCTTACTGCTGCAAACGCTCTGCAAGCTGTCAGAGAACATGAAAACCCTGTACGTCACTGGGGAAGAATCCT
TGCAGCAGGTGGCGATGCGGGCGCATCGTCTCAACCTGCCGACCCAAAATCTCAATATGCTGTCGGAAACCAGCATCGAA
CAGATTTGCCTGATTGCCGAGCAGGAACAGCCGAAGCTGATGGTGATCGACTCCATTCAGGTGATGCATCTCGCGGATAT
TCAATCGTCACCCGGCAGCGTGGCGCAGGTGCGTGAAACCGCCGCCTACCTGACGCGCTTTGCAAAAACGCGCGGCGTCG
CCATCGTGATGGTCGGCCACGTCACCAAAGACGGTTCACTCGCCGGACCAAAAGTATTGGAACACTGCATCGACTGCTCC
GTGCTGCTGGATGGCGATGCCGATTCGCGCTTCCGCACCCTGCGCAGCCATAAAAACCGCTTCGGTGCCGTTAACGAGCT
GGGCGTGTTCGCGATGACGGAACAGGGTCTACGCGAGATCAGCAATCCGTCGGCAATTTTCCTCAGTCGCGGTGACGAAA
TCACGTCCGGCAGCTCGGTCATGGTGGTATGGGAAGGTACCCGTCCGCTGCTGGTCGAAATTCAGGCGCTGGTGGATCAA
TCAATGATGGCTAACCCGCGTCGCGTGGCGGTCGGGCTGGAGCAAAATCGCCTGGCGATCCTGCTGGCGGTATTACATCG
CCATGGCGGCTTGCAGATGTCCGATCAGGATGTGTTCGTGAATGTCGTCGGCGGCGTTAAAGTCACCGAAACCAGCGCCG
ACCTGGCGCTGCTGCTATCGCTGGTCTCCAGCTTCCGCGACCGCCCGTTGCCGCAAGATCTTGTCATCTTCGGTGAAGTC
GGTCTGGCGGGTGAAATCCGCCCTGTTCCGAGCGGCCAAGAGCGGATTACCGAAGCCGCCAAGCACGGCTTCAAACGCGC
CATCGTTCCTCATGCCAATATGCCGAAGAAAGTCCCTGCCAGCATGCAGGTGTTCGGCGTGAAAAAGCTGGCCGATGCGC
TGGCGATCCTCGACGATCTCTAA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(89-225)

Subunit ChlI of Mg-chelatase (ChlI)

(348-433)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(9-36)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3S0ZXC5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

48.261

100

0.483
  radA Streptococcus pneumoniae Rx1

42.982

99.13

0.426
  radA Streptococcus pneumoniae D39

42.982

99.13

0.426
  radA Streptococcus pneumoniae R6

42.982

99.13

0.426
  radA Streptococcus pneumoniae TIGR4

42.982

99.13

0.426
  radA Streptococcus mitis NCTC 12261

42.982

99.13

0.426
  radA Streptococcus mitis SK321

42.982

99.13

0.426

Multiple sequence alignment