Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   DMB75_RS10435 Genome accession   NZ_CP068031
Coordinates   2164840..2166207 (-) Length   455 a.a.
NCBI ID   WP_130546807.1    Uniprot ID   -
Organism   Staphylococcus saccharolyticus strain DVP5-16-4677     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2159840..2171207
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DMB75_RS10420 (DMB75_010410) cysE 2160824..2161453 (-) 630 WP_199798468.1 serine O-acetyltransferase -
  DMB75_RS10425 (DMB75_010415) gltX 2161867..2163321 (-) 1455 WP_130546809.1 glutamate--tRNA ligase -
  DMB75_RS10430 (DMB75_010420) - 2163705..2164787 (-) 1083 WP_130546808.1 PIN/TRAM domain-containing protein -
  DMB75_RS10435 (DMB75_010425) radA 2164840..2166207 (-) 1368 WP_130546807.1 DNA repair protein RadA Machinery gene
  DMB75_RS10440 (DMB75_010430) - 2166441..2168894 (-) 2454 WP_130546806.1 ATP-dependent Clp protease ATP-binding subunit -
  DMB75_RS10445 (DMB75_010435) - 2168910..2169914 (-) 1005 WP_130546805.1 protein arginine kinase -
  DMB75_RS10450 (DMB75_010440) - 2169907..2170470 (-) 564 WP_130546804.1 UvrB/UvrC motif-containing protein -
  DMB75_RS10455 (DMB75_010445) - 2170491..2170952 (-) 462 WP_130546803.1 CtsR family transcriptional regulator -

Sequence

Protein


Download         Length: 455 a.a.        Molecular weight: 50036.76 Da        Isoelectric Point: 8.0158

>NTDB_id=526411 DMB75_RS10435 WP_130546807.1 2164840..2166207(-) (radA) [Staphylococcus saccharolyticus strain DVP5-16-4677]
MAKKKVIFECMACGYQSPKWMGKCPNCGAWNQMEETIEKATNPKHGVKSKKVVGKVQKLNSIKHEVIPRILTDSKEFNRV
LGGGIVSGSLVLIGGDPGIGKSTLLLQICATLSQNKNVLYITGEESINQTKLRADRLEEDSSHLNVLAETDLEVIHQTVK
DERPDLLVIDSIQTIYHPEISSAPGSVSQVRESTQSLMNIAKQMNIATFIVGHVTKEGQIAGPRLLEHMVDTVLYFEGDE
HHAYRILRAVKNRFGSTNEMGIFEMKQSGLKGVLNPSEMFLEERSTNVPGSTIVPTMEGTRPLLIEVQALVTPTTFNNPR
RMATGIDHNRLSLLMAVLEKKENYLLQQQDAYIKVAGGVKLTEPAVDLSIIVATASSFKDKAVDGLDCFVGEVGLTGEVR
RVSRIEQRVQEAAKLGFKRAIIPKTNIGGWTFPEGIQVIGVSTVHEALKFALHST

Nucleotide


Download         Length: 1368 bp        

>NTDB_id=526411 DMB75_RS10435 WP_130546807.1 2164840..2166207(-) (radA) [Staphylococcus saccharolyticus strain DVP5-16-4677]
ATGGCCAAGAAAAAAGTAATATTTGAATGTATGGCATGTGGCTATCAATCTCCGAAATGGATGGGTAAATGCCCAAATTG
TGGTGCATGGAATCAAATGGAAGAAACGATAGAGAAGGCTACCAACCCTAAGCATGGTGTAAAGTCAAAGAAAGTAGTAG
GAAAAGTTCAAAAGCTTAATAGTATTAAACATGAAGTCATACCGAGAATTCTTACTGATTCAAAAGAATTTAATAGAGTA
CTTGGTGGTGGAATCGTTAGTGGGTCGCTTGTACTTATAGGTGGAGATCCAGGTATAGGAAAATCTACACTACTTCTTCA
AATATGTGCCACACTATCGCAAAATAAAAATGTGCTATATATCACTGGTGAGGAATCGATTAATCAAACTAAACTACGCG
CAGATCGCTTAGAAGAAGATTCTAGTCACCTAAATGTATTAGCTGAAACTGATTTAGAGGTCATTCATCAAACCGTTAAA
GATGAACGTCCTGATTTGCTTGTCATAGACTCTATACAAACGATTTATCATCCGGAAATCAGTTCTGCGCCTGGTTCAGT
ATCTCAGGTTAGGGAAAGTACACAAAGTTTAATGAATATAGCGAAGCAGATGAATATTGCTACCTTTATAGTTGGTCATG
TTACGAAAGAAGGCCAAATAGCTGGGCCTAGATTACTTGAGCATATGGTAGATACAGTACTATATTTCGAAGGTGATGAG
CATCATGCTTATCGTATCTTGAGAGCCGTTAAAAACCGTTTTGGATCTACAAATGAGATGGGGATTTTTGAAATGAAACA
AAGTGGATTAAAAGGTGTTCTTAATCCATCTGAAATGTTCTTAGAGGAACGTTCAACTAATGTTCCTGGTTCGACAATTG
TTCCTACTATGGAAGGAACACGACCACTTCTTATCGAAGTTCAAGCATTAGTTACTCCTACAACATTTAATAATCCTAGA
CGTATGGCTACTGGTATAGACCACAATAGATTAAGTCTCCTGATGGCTGTATTAGAAAAGAAAGAAAATTATTTACTGCA
ACAACAAGACGCTTATATTAAAGTTGCAGGTGGTGTGAAATTAACAGAACCGGCTGTAGACCTTAGCATTATTGTAGCTA
CTGCATCAAGCTTCAAAGACAAAGCTGTAGACGGCTTAGACTGTTTTGTAGGTGAAGTAGGTTTGACTGGGGAAGTTCGT
CGAGTATCGCGTATAGAACAACGTGTTCAAGAAGCAGCAAAACTTGGTTTTAAAAGAGCTATCATTCCTAAAACTAATAT
AGGAGGGTGGACTTTTCCTGAAGGTATACAGGTAATTGGTGTATCTACAGTTCATGAAGCTTTGAAATTCGCACTTCACT
CGACATAA

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(353-426)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(77-218)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

66.886

100

0.67
  radA Streptococcus pneumoniae Rx1

61.47

98.681

0.607
  radA Streptococcus pneumoniae D39

61.47

98.681

0.607
  radA Streptococcus pneumoniae R6

61.47

98.681

0.607
  radA Streptococcus pneumoniae TIGR4

61.47

98.681

0.607
  radA Streptococcus mitis NCTC 12261

61.47

98.681

0.607
  radA Streptococcus mitis SK321

61.47

98.681

0.607