Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   DMB76_RS10470 Genome accession   NZ_CP068029
Coordinates   2154307..2155674 (-) Length   455 a.a.
NCBI ID   WP_115313969.1    Uniprot ID   A0A380HC52
Organism   Staphylococcus saccharolyticus strain 13T0028     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2149307..2160674
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DMB76_RS10455 (DMB76_010460) cysE 2150285..2150914 (-) 630 WP_185802520.1 serine O-acetyltransferase -
  DMB76_RS10460 (DMB76_010465) gltX 2151323..2152777 (-) 1455 WP_115313967.1 glutamate--tRNA ligase -
  DMB76_RS10465 (DMB76_010470) - 2153172..2154254 (-) 1083 WP_115313968.1 PIN/TRAM domain-containing protein -
  DMB76_RS10470 (DMB76_010475) radA 2154307..2155674 (-) 1368 WP_115313969.1 DNA repair protein RadA Machinery gene
  DMB76_RS10475 (DMB76_010480) - 2155908..2158361 (-) 2454 WP_115313970.1 ATP-dependent Clp protease ATP-binding subunit -
  DMB76_RS10480 (DMB76_010485) - 2158377..2159381 (-) 1005 WP_115313971.1 protein arginine kinase -
  DMB76_RS10485 (DMB76_010490) - 2159374..2159937 (-) 564 WP_115313972.1 UvrB/UvrC motif-containing protein -
  DMB76_RS10490 (DMB76_010495) - 2159958..2160419 (-) 462 WP_115313973.1 CtsR family transcriptional regulator -

Sequence

Protein


Download         Length: 455 a.a.        Molecular weight: 50126.76 Da        Isoelectric Point: 7.2804

>NTDB_id=526385 DMB76_RS10470 WP_115313969.1 2154307..2155674(-) (radA) [Staphylococcus saccharolyticus strain 13T0028]
MAKKKVIFECMACGYQSPKWMGKCPSCGAWNQMEETIEKATNPKHDVKSKEVVGKVQKLNSIKHEVIPRILTDSKEFNRV
LGGGIVSGSLVLIGGDPGIGKSTLLLQICATLSQNKNVLYISGEESINQTKLRADRLEEDSSHLNVLAETDLEVIHQTVK
DERPDLLVIDSIQTIYHPEISSAPGSVSQVRESTQSLMNIAKQMNIATFIVGHVTKEGQIAGPRLLEHMVDTVLYFEGDE
HHAYRILRAVKNRFGSTNEMGIFEMKQSGLKGVLNPSEMFLEERSTNVPGSTIVPTMEGTRPLLIEVQALVTPTTFNNPR
RMATGIDHNRLSLLMAVLEKKENYLLQQQDAYIKVAGGVKLTEPAVDLSIIVATASSFKDRTVDGLDCFVGEVGLTGEVR
RISRIEQRVQEAAKLGFKRAIIPKTNIGGWTFPEGIQVIGVSTVHEALKFALHST

Nucleotide


Download         Length: 1368 bp        

>NTDB_id=526385 DMB76_RS10470 WP_115313969.1 2154307..2155674(-) (radA) [Staphylococcus saccharolyticus strain 13T0028]
ATGGCCAAGAAAAAAGTAATATTTGAATGTATGGCATGTGGCTATCAATCTCCGAAATGGATGGGTAAATGCCCAAGTTG
TGGTGCATGGAATCAAATGGAAGAAACGATAGAGAAGGCTACCAACCCTAAGCATGATGTAAAGTCAAAGGAAGTAGTAG
GAAAAGTTCAAAAGCTTAATAGTATTAAACATGAAGTCATACCGAGAATTCTTACTGATTCAAAAGAATTTAATAGAGTA
CTTGGTGGTGGAATCGTTAGTGGGTCGCTTGTACTTATAGGTGGAGATCCAGGTATAGGAAAGTCTACACTACTTCTTCA
AATATGTGCTACACTATCGCAAAATAAAAATGTGCTATATATCAGTGGTGAGGAATCGATTAATCAAACTAAACTACGCG
CAGATCGCTTAGAAGAAGATTCTAGTCACCTAAATGTATTAGCTGAAACTGATTTAGAGGTCATTCATCAAACCGTTAAA
GATGAACGTCCTGATTTGCTTGTCATAGACTCTATACAAACGATTTATCATCCGGAAATCAGTTCTGCGCCTGGTTCAGT
ATCTCAGGTTAGGGAAAGCACACAAAGTTTAATGAATATAGCGAAGCAGATGAATATTGCTACCTTTATAGTTGGTCATG
TTACGAAAGAAGGCCAAATAGCTGGGCCTAGATTACTTGAGCATATGGTAGATACAGTACTATATTTCGAAGGTGATGAG
CATCATGCTTATCGTATCTTGAGAGCCGTTAAAAACCGTTTTGGATCTACAAATGAGATGGGGATTTTTGAAATGAAACA
AAGTGGATTAAAAGGTGTTCTTAATCCATCTGAAATGTTCTTAGAGGAACGTTCAACTAATGTTCCTGGTTCGACAATTG
TTCCTACTATGGAAGGAACACGACCACTTCTTATCGAAGTTCAAGCATTAGTTACTCCTACAACATTTAATAATCCTAGA
CGTATGGCTACTGGTATAGATCACAATAGATTAAGTCTCCTGATGGCTGTATTAGAAAAGAAAGAAAATTATTTACTGCA
ACAACAAGACGCTTATATTAAAGTTGCAGGTGGTGTGAAATTAACAGAACCGGCTGTAGACCTTAGCATTATTGTAGCTA
CTGCATCAAGCTTCAAAGACAGAACTGTAGACGGCTTAGACTGTTTTGTAGGTGAAGTAGGTTTGACTGGGGAAGTTCGT
CGAATATCGCGTATAGAACAACGTGTTCAAGAAGCAGCAAAACTTGGTTTTAAAAGAGCTATCATTCCTAAAACTAATAT
AGGAGGTTGGACTTTTCCTGAAGGTATACAGGTAATTGGTGTATCTACAGTTCATGAAGCTTTGAAATTCGCACTTCACT
CGACATAA

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(353-426)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(77-218)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A380HC52

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

66.886

100

0.67
  radA Streptococcus pneumoniae Rx1

61.024

98.681

0.602
  radA Streptococcus pneumoniae D39

61.024

98.681

0.602
  radA Streptococcus pneumoniae R6

61.024

98.681

0.602
  radA Streptococcus pneumoniae TIGR4

61.024

98.681

0.602
  radA Streptococcus mitis NCTC 12261

61.024

98.681

0.602
  radA Streptococcus mitis SK321

61.024

98.681

0.602