Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   K6124_RS00560 Genome accession   NZ_CP082103
Coordinates   119925..120365 (-) Length   146 a.a.
NCBI ID   WP_000360915.1    Uniprot ID   A0A0H2V436
Organism   Escherichia coli strain MS15046     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 114925..125365
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K6124_RS00535 (K6124_00525) coaE 115415..116035 (-) 621 WP_001269525.1 dephospho-CoA kinase -
  K6124_RS00540 (K6124_00530) - 116060..116104 (+) 45 WP_120795372.1 protein YacM -
  K6124_RS00545 (K6124_00535) guaC 116260..117303 (+) 1044 WP_001217338.1 GMP reductase -
  K6124_RS00550 (K6124_00540) hofC 117338..118540 (-) 1203 WP_000157242.1 protein transport protein HofC -
  K6124_RS00555 (K6124_00545) pilB 118530..119915 (-) 1386 WP_001025180.1 type II secretion system protein GspE Machinery gene
  K6124_RS00560 (K6124_00550) pilA 119925..120365 (-) 441 WP_000360915.1 prepilin peptidase-dependent pilin Machinery gene
  K6124_RS00565 (K6124_00555) nadC 120569..121462 (-) 894 WP_001135168.1 carboxylating nicotinate-nucleotide diphosphorylase -
  K6124_RS00570 (K6124_00560) ampD 121550..122101 (+) 552 WP_000923726.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  K6124_RS00575 (K6124_00565) ampE 122098..122952 (+) 855 WP_000172013.1 beta-lactamase regulator AmpE -
  K6124_RS00580 (K6124_00570) aroP 122995..124365 (-) 1371 WP_000399017.1 aromatic amino acid transporter AroP -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15607.77 Da        Isoelectric Point: 4.5237

>NTDB_id=526183 K6124_RS00560 WP_000360915.1 119925..120365(-) (pilA) [Escherichia coli strain MS15046]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLNTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCTIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=526183 K6124_RS00560 WP_000360915.1 119925..120365(-) (pilA) [Escherichia coli strain MS15046]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAAATACCTGCGACGGTGGCAGCAATGGCATTCCTTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGGCTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGTAACTGCACTATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2V436

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363