Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   B657_RS13930 Genome accession   NC_018520
Coordinates   2631859..2632269 (-) Length   136 a.a.
NCBI ID   WP_009967785.1    Uniprot ID   A0A6I4D881
Organism   Bacillus subtilis QB928     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2626859..2637269
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  B657_RS13895 - 2626946..2627086 (+) 141 WP_003226124.1 sporulation histidine kinase inhibitor Sda -
  B657_RS13905 yqeF 2627392..2628123 (-) 732 WP_003229964.1 SGNH/GDSL hydrolase family protein -
  B657_RS13910 cwlH 2628375..2629127 (-) 753 WP_003229963.1 N-acetylmuramoyl-L-alanine amidase CwlH -
  B657_RS13915 yqeD 2629314..2629940 (+) 627 WP_003229962.1 TVP38/TMEM64 family protein -
  B657_RS13920 gnd 2629959..2630852 (-) 894 WP_003229961.1 phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) -
  B657_RS13925 yqeB 2631104..2631826 (+) 723 WP_010886572.1 hypothetical protein -
  B657_RS13930 nucA/comI 2631859..2632269 (-) 411 WP_009967785.1 sporulation-specific Dnase NucB Machinery gene
  B657_RS13935 sigK 2632465..2633193 (+) 729 WP_013308023.1 RNA polymerase sporulation sigma factor SigK -
  B657_RS22500 - 2633193..2633309 (+) 117 WP_003229898.1 hypothetical protein -
  B657_RS22055 - 2633275..2633475 (-) 201 Protein_2675 recombinase family protein -
  B657_RS22060 (B657_26400) - 2633418..2633582 (-) 165 WP_010886576.1 hypothetical protein -
  B657_RS13945 (B657_26410) psiE 2634127..2634543 (-) 417 WP_003246154.1 phosphate-starvation-inducible protein PsiE -
  B657_RS13950 (B657_26420) yrkQ 2634589..2635887 (-) 1299 WP_003246194.1 two-component system sensor histidine kinase YrkQ -
  B657_RS13955 (B657_26430) yrkP 2635874..2636569 (-) 696 WP_003229895.1 two-component response regulator YrkP -

Sequence

Protein


Download         Length: 136 a.a.        Molecular weight: 14967.97 Da        Isoelectric Point: 5.1853

>NTDB_id=52615 B657_RS13930 WP_009967785.1 2631859..2632269(-) (nucA/comI) [Bacillus subtilis QB928]
MKKWMAGLFLAAAVLLCLMVPQQIQGASSYDKVLYFPLSRYPETGSHIRDAIAEGHPDICTIDRDGADKRREESLKGIPT
KPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSSYPDGTRVLFIVQ

Nucleotide


Download         Length: 411 bp        

>NTDB_id=52615 B657_RS13930 WP_009967785.1 2631859..2632269(-) (nucA/comI) [Bacillus subtilis QB928]
ATGAAAAAATGGATGGCAGGCCTGTTTCTTGCTGCAGCAGTTCTTCTTTGTTTAATGGTTCCGCAACAGATCCAAGGCGC
ATCTTCGTATGACAAAGTGTTATATTTTCCGCTGTCTCGTTATCCGGAAACCGGCAGTCATATTAGGGATGCGATTGCAG
AGGGACATCCAGATATTTGTACCATTGACAGAGATGGAGCAGACAAAAGGCGGGAGGAATCTTTAAAGGGAATCCCGACC
AAGCCGGGCTATGACCGGGATGAGTGGCCGATGGCGGTCTGCGAGGAAGGCGGTGCAGGGGCTGATGTCCGATATGTGAC
GCCTTCTGATAATCGCGGCGCCGGCTCGTGGGTAGGGAATCAAATGAGCAGCTATCCTGACGGTACCAGAGTGCTGTTTA
TTGTGCAGTAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(40-130)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6I4D881

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

62.609

84.559

0.529

Multiple sequence alignment