Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   JJL53_RS20545 Genome accession   NZ_CP067417
Coordinates   4425658..4427097 (-) Length   479 a.a.
NCBI ID   WP_041204880.1    Uniprot ID   -
Organism   Aeromonas media strain E31     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4420658..4432097
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JJL53_RS20515 (JJL53_20515) degS 4421411..4422532 (+) 1122 WP_041204883.1 outer membrane-stress sensor serine endopeptidase DegS -
  JJL53_RS25200 (JJL53_20520) - 4422635..4423682 (+) 1048 Protein_3975 PTS sugar transporter subunit IIA -
  JJL53_RS20525 (JJL53_20525) npr 4423701..4423973 (-) 273 WP_005331359.1 PTS phosphocarrier protein NPr -
  JJL53_RS20530 (JJL53_20530) rapZ 4423994..4424860 (-) 867 WP_041204881.1 RNase adapter RapZ -
  JJL53_RS20535 (JJL53_20535) ptsN 4424901..4425347 (-) 447 WP_005331355.1 PTS IIA-like nitrogen regulatory protein PtsN -
  JJL53_RS20540 (JJL53_20540) hpf 4425350..4425637 (-) 288 WP_005305957.1 ribosome hibernation promoting factor -
  JJL53_RS20545 (JJL53_20545) rpoN/rpoN1 4425658..4427097 (-) 1440 WP_041204880.1 RNA polymerase factor sigma-54 Machinery gene
  JJL53_RS20550 (JJL53_20550) lptB 4427163..4427888 (-) 726 WP_010675872.1 LPS export ABC transporter ATP-binding protein -
  JJL53_RS20555 (JJL53_20555) lptA 4427901..4428419 (-) 519 WP_041204879.1 lipopolysaccharide transport periplasmic protein LptA -
  JJL53_RS20560 (JJL53_20560) lptC 4428406..4428966 (-) 561 WP_005331338.1 LPS export ABC transporter periplasmic protein LptC -
  JJL53_RS20565 (JJL53_20565) kdsC 4428963..4429517 (-) 555 WP_005331333.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  JJL53_RS20570 (JJL53_20570) - 4429517..4430512 (-) 996 WP_201035265.1 KpsF/GutQ family sugar-phosphate isomerase -
  JJL53_RS20575 (JJL53_20575) mlaF 4430719..4431519 (+) 801 WP_005331323.1 phospholipid ABC transporter ATP-binding protein MlaF -

Sequence

Protein


Download         Length: 479 a.a.        Molecular weight: 53771.74 Da        Isoelectric Point: 4.4457

>NTDB_id=526029 JJL53_RS20545 WP_041204880.1 4425658..4427097(-) (rpoN/rpoN1) [Aeromonas media strain E31]
MKPTLQLRLGQSLTMTPQLQQAIRLLQLSTLELQQEIQQALDNNPLLEQEENEAHETSPIEPQDASQLDSSDAMAQDKMP
DELPVDTTWDEVYSAGTAQSAGPIHDGEDSVYQGETTENLQDYLLWQMRLTPFSDLDAAIALAIIDAIDESGYLTVSLED
IQAAVSSEEVEVELDEVEAVLKRVQHFDPVGIAARSVQECLLIQLGQYSPDLPWLNEAREVIREHMDLLGNRDYRTLARK
TRLKESDLKEVLDLIQQLEPRPGNGIIQSESQYVIPDVSVVKKGAKWVVELNPDSAPRIRVNEAYAAMARNARSSTDTQF
IRSHLQEAKWFIKSLESRNDTLLKVASCIVEQQQGFFEYGEEAMKPMVLNDIAEAVEMHESTISRVTTQKYMHTPRGIFE
LKFFFSSHVNTEGGGECSSTAIRALIKKLVTAENPAKPLSDSKIADLLAEQGIMVARRTIAKYRESLLIPPSNQRKRLV

Nucleotide


Download         Length: 1440 bp        

>NTDB_id=526029 JJL53_RS20545 WP_041204880.1 4425658..4427097(-) (rpoN/rpoN1) [Aeromonas media strain E31]
ATGAAGCCGACATTACAGTTGCGTCTCGGCCAGTCCCTGACCATGACGCCACAATTACAACAAGCGATTCGTCTGCTCCA
GCTCTCCACCCTGGAACTCCAGCAGGAAATTCAGCAAGCGCTCGATAATAACCCCTTGCTGGAGCAGGAGGAGAATGAGG
CGCACGAAACCAGTCCGATAGAGCCCCAGGACGCCAGCCAACTCGACTCCAGTGACGCAATGGCACAGGACAAGATGCCG
GATGAACTGCCGGTGGACACCACCTGGGATGAGGTCTATTCCGCCGGCACGGCTCAGAGCGCTGGCCCCATCCATGACGG
GGAAGACAGCGTCTATCAGGGGGAAACCACCGAAAATCTGCAGGATTATCTGTTGTGGCAGATGCGACTGACGCCGTTCA
GTGATCTGGATGCCGCGATTGCCCTCGCCATCATCGATGCCATCGATGAGTCTGGCTACCTGACCGTTTCTCTCGAAGAT
ATTCAGGCTGCAGTCAGCAGTGAAGAGGTAGAAGTTGAACTGGATGAAGTGGAAGCCGTGCTCAAGCGGGTGCAGCATTT
CGATCCGGTTGGCATCGCCGCTCGCTCGGTACAGGAGTGTCTGTTGATCCAGCTTGGCCAGTATTCGCCGGATCTGCCTT
GGCTCAATGAGGCAAGGGAGGTGATCCGCGAGCACATGGACTTGCTCGGCAACCGTGACTACCGCACCCTGGCGCGCAAG
ACCCGGCTCAAGGAGAGCGACCTCAAGGAGGTACTGGATCTCATCCAGCAGCTGGAGCCCAGACCCGGTAATGGCATCAT
CCAGAGCGAATCGCAATACGTGATCCCGGATGTTTCCGTGGTCAAGAAGGGGGCGAAGTGGGTGGTTGAACTCAACCCGG
ATTCGGCGCCGCGCATTCGTGTCAACGAGGCCTATGCGGCAATGGCACGCAATGCCCGCAGCAGCACGGATACTCAGTTC
ATTCGTTCCCATTTGCAGGAAGCCAAGTGGTTCATCAAGAGTCTGGAGAGTCGAAACGACACCCTGCTCAAGGTGGCAAG
CTGCATAGTCGAGCAGCAACAGGGTTTCTTCGAGTACGGTGAAGAGGCCATGAAGCCCATGGTACTCAACGACATCGCCG
AGGCGGTGGAGATGCACGAGTCGACGATTTCACGGGTGACGACCCAGAAGTACATGCATACGCCCCGTGGCATCTTTGAA
CTGAAGTTTTTTTTCTCCAGTCATGTCAACACGGAGGGAGGAGGAGAGTGCTCATCGACCGCAATTCGTGCCCTTATCAA
GAAGCTGGTGACGGCCGAAAACCCGGCCAAACCGCTTAGTGATAGCAAGATCGCGGATTTGCTGGCCGAACAGGGTATTA
TGGTGGCGAGACGCACGATCGCAAAGTACCGTGAATCCTTGTTGATCCCGCCATCCAATCAGCGCAAACGCCTGGTTTAG

Domains

Predicted by InterproScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(319-477)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-305)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

46.667

100

0.497