Detailed information    

insolico Bioinformatically predicted

Overview

Name   sbcB   Type   Machinery gene
Locus tag   K6127_RS10435 Genome accession   NZ_CP082085
Coordinates   2138027..2139454 (+) Length   475 a.a.
NCBI ID   WP_021522136.1    Uniprot ID   -
Organism   Escherichia coli strain MS20653     
Function   promote homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2133027..2144454
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K6127_RS10395 (K6127_10350) yeeW 2133212..2133406 (+) 195 WP_000988601.1 DUF5983 family protein -
  K6127_RS10400 (K6127_10355) - 2133452..2133532 (+) 81 Protein_2042 hypothetical protein -
  K6127_RS10405 (K6127_10360) - 2133821..2133967 (-) 147 Protein_2043 transposase domain-containing protein -
  K6127_RS10410 (K6127_10365) - 2134021..2134203 (+) 183 WP_001016348.1 EutP/PduV family microcompartment system protein -
  K6127_RS10415 (K6127_10370) tmaR 2134304..2134633 (-) 330 WP_000450409.1 DUF496 family protein -
  K6127_RS10420 (K6127_10375) yeeA 2134805..2135863 (-) 1059 WP_001200890.1 FUSC family protein -
  K6127_RS10425 (K6127_10380) sbmC 2136061..2136534 (-) 474 WP_001105376.1 DNA gyrase inhibitor SbmC -
  K6127_RS10430 (K6127_10385) dacD 2136652..2137818 (-) 1167 WP_001297905.1 serine-type D-Ala-D-Ala carboxypeptidase DacD -
  K6127_RS10435 (K6127_10390) sbcB 2138027..2139454 (+) 1428 WP_021522136.1 exodeoxyribonuclease I Machinery gene
  K6127_RS10440 (K6127_10395) plaP 2139528..2140886 (-) 1359 WP_000019194.1 putrescine/proton symporter PlaP -
  K6127_RS10445 (K6127_10400) yoeI 2140876..2140938 (-) 63 WP_010723108.1 membrane protein YoeI -
  K6127_RS10450 (K6127_10405) yeeY 2141152..2142081 (-) 930 WP_000803366.1 LysR substrate-binding domain-containing protein -
  K6127_RS10455 (K6127_10410) yeeZ 2142127..2142951 (-) 825 WP_000754737.1 SDR family oxidoreductase -
  K6127_RS10460 (K6127_10415) yoeB 2143034..2143288 (-) 255 WP_000767822.1 type II toxin-antitoxin system mRNA interferase toxin YoeB -
  K6127_RS10465 (K6127_10420) yefM 2143285..2143536 (-) 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM -
  K6127_RS10470 (K6127_10425) hisL 2143819..2143869 (+) 51 WP_001364200.1 his operon leader peptide -

Sequence

Protein


Download         Length: 475 a.a.        Molecular weight: 54456.76 Da        Isoelectric Point: 5.4720

>NTDB_id=525700 K6127_RS10435 WP_021522136.1 2138027..2139454(+) (sbcB) [Escherichia coli strain MS20653]
MMNDGKQQSTFLFHDYETFGTHPALDRPAQFAAIRTDSEFNVIGEPEVFYCKPADDYLPQPGAVLITGITPQEARAKGEN
EAAFAARIHSLFTVPKTCILGYNNVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDG
LPSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLMALIDVPQMKPLVHVSGMFGAWR
GNTTWVAPLAWHPENRNAVIMVDLAGDISPLLELDSDTLRERLYTAKADLGDNAAVPVKLVHINKCPVLAQANTLRPEDA
DRLGINRQHCLDNLKILRENPQVREKVVAIFAEAEPFTPSENVDAQLYNGFFSDADRAAMKIVLETEPRNLPALDITFVD
KRIEKLLFNYRARNFPGTLDYAEQQRWLEHRRQVFTPEFLQGYADELQMLAQQYADDKEKVALLKALWQYAEEIV

Nucleotide


Download         Length: 1428 bp        

>NTDB_id=525700 K6127_RS10435 WP_021522136.1 2138027..2139454(+) (sbcB) [Escherichia coli strain MS20653]
ATGATGAATGACGGTAAGCAACAATCTACCTTTTTGTTTCACGATTACGAAACCTTTGGCACGCACCCCGCGTTAGATCG
CCCTGCACAGTTCGCAGCCATTCGCACCGATAGCGAATTCAATGTCATCGGCGAACCCGAAGTCTTTTACTGCAAGCCCG
CGGATGACTATTTACCCCAGCCTGGAGCAGTATTAATTACCGGTATTACCCCGCAGGAAGCTCGGGCGAAAGGAGAAAAC
GAAGCCGCATTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTT
CGACGACGAAGTCACACGCAACGTTTTTTATCGTAATTTCTACGATCCTTACGCCTGGAGCTGGCAGCATGATAACTCGC
GCTGGGATTTACTGGATGTTATGCGTGCCTGTTATGCCCTGCGCCCGGAAGGAATAAACTGGCCTGAAAATGATGACGGT
CTACCGAGCTTTCGCCTTGAGCATTTAACCAAAGCGAATGGTATTGAACATAGCAACGCCCACGATGCGATGGCTGATGT
GTACGCCACTATTGCGATGGCGAAACTGGTAAAAACGCGTCAGCCACGCCTGTTTGATTATCTCTTTACCCATCGTAATA
AACACAAACTGATGGCGTTGATTGATGTTCCGCAGATGAAACCCCTGGTTCACGTTTCCGGAATGTTTGGGGCATGGCGC
GGCAATACCACCTGGGTGGCACCGCTGGCGTGGCATCCTGAAAATCGCAATGCCGTAATTATGGTGGATTTGGCAGGAGA
CATTTCGCCATTACTGGAGCTGGATAGCGACACATTGCGCGAGCGTTTATATACCGCAAAAGCCGATCTTGGCGATAACG
CCGCCGTTCCGGTTAAGCTGGTGCATATCAATAAATGTCCGGTACTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCC
GACCGACTGGGAATTAATCGTCAGCATTGCCTCGATAACCTAAAAATTCTGCGTGAAAATCCGCAAGTGCGTGAAAAAGT
GGTGGCGATATTCGCGGAAGCCGAACCGTTTACGCCTTCAGAGAACGTGGATGCACAGCTTTATAATGGCTTTTTCAGTG
ACGCCGATCGTGCAGCAATGAAAATTGTGCTGGAAACCGAGCCGCGTAATTTACCGGCACTGGATATCACTTTTGTTGAT
AAACGAATTGAAAAACTGTTGTTCAATTATCGAGCACGCAACTTCCCGGGGACGCTGGATTATGCCGAGCAGCAACGCTG
GCTGGAGCACCGCCGCCAGGTCTTCACGCCAGAGTTTTTGCAGGGCTACGCTGATGAATTGCAGATGCTGGCACAACAGT
ATGCCGATGACAAAGAGAAAGTGGCGCTGTTAAAAGCGCTTTGGCAGTACGCGGAAGAGATCGTCTGA

Domains

Predicted by InterProScan.

Exonuclease C-terminal (ExoI_SH3)

(212-472)

Exonuclease (RNase_T)

(13-188)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  sbcB Vibrio cholerae O1 biovar El Tor strain E7946

58.723

98.947

0.581