Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   K6133_RS00560 Genome accession   NZ_CP082083
Coordinates   119243..119683 (-) Length   146 a.a.
NCBI ID   WP_000360914.1    Uniprot ID   A0A0H2YVZ3
Organism   Escherichia coli strain MS20654     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 114243..124683
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K6133_RS00535 (K6133_00530) coaE 114733..115353 (-) 621 WP_001269535.1 dephospho-CoA kinase -
  K6133_RS00540 (K6133_00535) - 115378..115422 (+) 45 WP_120795372.1 protein YacM -
  K6133_RS00545 (K6133_00540) guaC 115578..116621 (+) 1044 WP_001217338.1 GMP reductase -
  K6133_RS00550 (K6133_00545) hofC 116656..117858 (-) 1203 WP_000157254.1 protein transport protein HofC -
  K6133_RS00555 (K6133_00550) pilB 117848..119233 (-) 1386 WP_001025178.1 type II secretion system protein GspE Machinery gene
  K6133_RS00560 (K6133_00555) pilA 119243..119683 (-) 441 WP_000360914.1 prepilin peptidase-dependent pilin Machinery gene
  K6133_RS00565 (K6133_00560) nadC 119887..120780 (-) 894 WP_001135168.1 carboxylating nicotinate-nucleotide diphosphorylase -
  K6133_RS00570 (K6133_00565) ampD 120868..121419 (+) 552 WP_000923703.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  K6133_RS00575 (K6133_00570) ampE 121416..122270 (+) 855 WP_000171983.1 beta-lactamase regulator AmpE -
  K6133_RS00580 (K6133_00575) aroP 122313..123683 (-) 1371 WP_000399017.1 aromatic amino acid transporter AroP -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15620.77 Da        Isoelectric Point: 4.5237

>NTDB_id=525593 K6133_RS00560 WP_000360914.1 119243..119683(-) (pilA) [Escherichia coli strain MS20654]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLNTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=525593 K6133_RS00560 WP_000360914.1 119243..119683(-) (pilA) [Escherichia coli strain MS20654]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAAATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGACTAAGCGTCGTCAT
GACGCCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGTAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2YVZ3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363