Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   I8J47_RS06300 Genome accession   NZ_CP067362
Coordinates   1258201..1259574 (-) Length   457 a.a.
NCBI ID   WP_003723898.1    Uniprot ID   L8DQ47
Organism   Listeria monocytogenes strain LM18     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1253201..1264574
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I8J47_RS06275 (I8J47_01254) epsC 1253303..1253917 (-) 615 WP_003726845.1 serine O-acetyltransferase EpsC -
  I8J47_RS06280 (I8J47_01255) gltX 1254317..1255792 (-) 1476 WP_003728082.1 glutamate--tRNA ligase -
  I8J47_RS06285 (I8J47_01256) ispF 1255811..1256284 (-) 474 WP_003728083.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  I8J47_RS06290 (I8J47_01257) ispD 1256277..1256975 (-) 699 WP_003728084.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  I8J47_RS06295 (I8J47_01258) - 1256995..1258068 (-) 1074 WP_003726425.1 PIN/TRAM domain-containing protein -
  I8J47_RS06300 (I8J47_01259) radA 1258201..1259574 (-) 1374 WP_003723898.1 DNA repair protein RadA Machinery gene
  I8J47_RS06305 (I8J47_01260) - 1259721..1262183 (-) 2463 WP_003726424.1 ATP-dependent Clp protease ATP-binding subunit -
  I8J47_RS06310 (I8J47_01261) - 1262212..1263234 (-) 1023 WP_031644030.1 protein arginine kinase -
  I8J47_RS06315 (I8J47_01262) - 1263231..1263749 (-) 519 WP_031644028.1 UvrB/UvrC motif-containing protein -
  I8J47_RS06320 (I8J47_01263) - 1263762..1264220 (-) 459 WP_003726421.1 CtsR family transcriptional regulator -

Sequence

Protein


Download         Length: 457 a.a.        Molecular weight: 49991.63 Da        Isoelectric Point: 7.9613

>NTDB_id=525574 I8J47_RS06300 WP_003723898.1 1258201..1259574(-) (radA) [Listeria monocytogenes strain LM18]
MAKAKRTTKFVCQACGYESAKWMGKCPNCNEWNQMVEALEPSKKSRSAFNHTGEPSKATPITQIASETEKRVETNMPELN
RVLGGGVVPGSMVLVGGDPGIGKSTLLLQVSAQLTLTNKKVLYISGEESIKQTKLRAERLQVSGDNLYVYAETNLEAVQE
TIDFVKPDFVVIDSIQTVYHPDVTSAAGSVSQVRECTATLMRIAKMQNIAIFIVGHVTKEGAIAGPRLLEHMVDTVLYFE
GERHHAYRILRAVKNRFGSTNEMGIFEMRDVGLVEVANPSEVFLEERLEGASGSTVVASMEGTRPVLVEIQALVSPTMFG
NAKRMATGIDYNKVSLIMAVLEKRVGLMLQNQDAYLKAAGGVKLDEPAVDLAVAVSVASSYRDKPTRSTDCFIGELGLTG
EIRRVARIEQRVQEAAKLGFKRIFIPKNNEGNWKIPKDVQVVGVETIGEALKKALPD

Nucleotide


Download         Length: 1374 bp        

>NTDB_id=525574 I8J47_RS06300 WP_003723898.1 1258201..1259574(-) (radA) [Listeria monocytogenes strain LM18]
ATGGCTAAAGCAAAAAGGACAACCAAATTTGTGTGTCAGGCATGTGGATATGAATCGGCAAAATGGATGGGAAAATGTCC
GAATTGCAACGAGTGGAATCAGATGGTAGAAGCTTTGGAGCCGTCAAAAAAATCACGCTCAGCTTTTAATCATACGGGAG
AACCTTCGAAAGCGACTCCGATTACTCAAATAGCAAGTGAAACAGAAAAACGTGTCGAAACCAATATGCCGGAGTTAAAT
AGAGTTCTTGGTGGAGGTGTGGTTCCAGGATCTATGGTACTTGTCGGTGGGGATCCTGGTATCGGGAAGTCGACTTTATT
ACTGCAAGTTTCAGCTCAACTGACGCTCACAAATAAAAAAGTATTGTATATATCAGGAGAAGAATCTATCAAACAAACGA
AGCTGCGGGCGGAACGCTTGCAAGTTTCGGGAGATAATTTATATGTGTATGCAGAAACAAATTTAGAAGCAGTTCAAGAA
ACGATTGATTTTGTGAAGCCTGATTTTGTAGTCATTGACTCCATTCAGACTGTTTATCATCCCGATGTTACAAGTGCAGC
GGGGAGTGTTTCGCAAGTTAGGGAATGTACAGCGACATTGATGCGAATTGCTAAAATGCAAAACATTGCAATCTTTATTG
TTGGGCATGTAACGAAGGAAGGTGCTATTGCGGGGCCGCGCCTACTTGAACATATGGTTGATACTGTGCTTTATTTTGAA
GGGGAGCGTCATCATGCTTACCGGATTTTGCGTGCAGTGAAAAACCGTTTTGGTTCCACTAATGAAATGGGTATATTTGA
AATGCGAGATGTTGGACTTGTAGAAGTTGCTAATCCTTCCGAAGTGTTCTTGGAAGAACGTCTTGAAGGCGCTTCAGGTT
CAACGGTTGTAGCTTCTATGGAAGGAACTCGCCCCGTCCTTGTGGAAATACAAGCGCTTGTTTCGCCTACGATGTTTGGT
AATGCTAAGCGAATGGCGACAGGAATAGACTATAATAAAGTTTCGCTAATTATGGCTGTTTTAGAAAAGCGAGTAGGTTT
AATGTTGCAAAACCAAGATGCTTATTTGAAAGCAGCTGGTGGGGTTAAATTGGATGAACCTGCAGTGGATTTGGCGGTTG
CAGTCAGTGTTGCATCTAGCTATCGTGATAAACCAACAAGAAGTACGGATTGCTTTATTGGAGAACTTGGACTTACAGGT
GAGATTCGTCGTGTTGCAAGAATTGAACAACGCGTACAAGAAGCCGCAAAACTTGGCTTTAAGCGAATTTTTATTCCTAA
AAATAACGAAGGAAATTGGAAAATACCGAAAGACGTGCAAGTGGTTGGGGTGGAAACGATTGGAGAAGCTTTGAAGAAAG
CTTTACCAGATTAA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(64-221)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(10-37)

Subunit ChlI of Mg-chelatase (ChlI)

(363-429)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB L8DQ47

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

68.421

99.781

0.683
  radA Streptococcus pneumoniae Rx1

65.265

98.906

0.646
  radA Streptococcus pneumoniae D39

65.265

98.906

0.646
  radA Streptococcus pneumoniae R6

65.265

98.906

0.646
  radA Streptococcus pneumoniae TIGR4

65.265

98.906

0.646
  radA Streptococcus mitis NCTC 12261

65.265

98.906

0.646
  radA Streptococcus mitis SK321

65.044

98.906

0.643