Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   GEM_RS03215 Genome accession   NC_018513
Coordinates   719170..720675 (-) Length   501 a.a.
NCBI ID   WP_014896015.1    Uniprot ID   -
Organism   Burkholderia cepacia GG4     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 714170..725675
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GEM_RS03190 (GEM_0645) - 715196..715831 (-) 636 WP_014896011.1 LON peptidase substrate-binding domain-containing protein -
  GEM_RS03195 (GEM_0646) rapZ 715858..716766 (-) 909 WP_014896012.1 RNase adapter RapZ -
  GEM_RS03200 (GEM_0647) hprK 716845..717813 (-) 969 WP_014896013.1 HPr(Ser) kinase/phosphatase -
  GEM_RS03205 (GEM_0648) - 717943..718482 (-) 540 WP_014896014.1 PTS sugar transporter subunit IIA -
  GEM_RS03210 (GEM_0649) hpf 718651..719010 (-) 360 WP_006477786.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  GEM_RS03215 (GEM_0650) rpoN/rpoN1 719170..720675 (-) 1506 WP_014896015.1 RNA polymerase factor sigma-54 Machinery gene
  GEM_RS03220 (GEM_0651) lptB 720835..721611 (-) 777 WP_014896016.1 LPS export ABC transporter ATP-binding protein -
  GEM_RS03225 (GEM_0652) lptA 721608..722273 (-) 666 WP_014896017.1 lipopolysaccharide transport periplasmic protein LptA -
  GEM_RS03230 (GEM_0653) lptC 722294..722908 (-) 615 WP_014896018.1 LPS export ABC transporter periplasmic protein LptC -
  GEM_RS03235 (GEM_0654) - 722912..723448 (-) 537 WP_014896019.1 KdsC family phosphatase -
  GEM_RS03240 (GEM_0655) kdsD 723448..724431 (-) 984 WP_014896020.1 arabinose 5-phosphate isomerase KdsD -

Sequence

Protein


Download         Length: 501 a.a.        Molecular weight: 54834.00 Da        Isoelectric Point: 4.8190

>NTDB_id=52514 GEM_RS03215 WP_014896015.1 719170..720675(-) (rpoN/rpoN1) [Burkholderia cepacia GG4]
MKASLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVAMAIAQNPLLENDDEWIASPLRVAADGSLIAQTPPAQSTESSV
GNSSSQSSDRAERDEPAAADEYDGYASSDAGDGPQWNLDEFGRSSGASDDDDLPPLQVQEAATSLRDHLSAQLRVTQASP
RDRALVMFLIESLDDDGYLTATLDEVLADLPSELEIDCDEMNAALALLHSFDPAGVGARSASECLKLQLLRLDPSPTRTL
ALEIVSQHLELLAARDFTRLRKQLKASDDALRDAHALIRSLEPFPGAAYGKAEADYVVPDIIVKKAGQGWLAELNPEVMP
RLRINNLYANILRNSRGDPAAGSLKQQLQEARWLIKNIQQRFDTILRVAQAIVERQKNFFAHGEIAMRPLVLREIADTLG
LHESTVSRVTTGKYMLTPFGTLEFKYFFGSHVSTDTGGAASSTAIRALIKQLIGAEDPKSPLSDSRIAELLAEQGFVVAR
RTVAKYREALKIPAVNLRKSL

Nucleotide


Download         Length: 1506 bp        

>NTDB_id=52514 GEM_RS03215 WP_014896015.1 719170..720675(-) (rpoN/rpoN1) [Burkholderia cepacia GG4]
ATGAAAGCCAGCCTTCAACTCCGCCTGTCGCAACACTTGGCGCTGACGCCGCAACTGCAGCAGTCGATCCGGCTTCTGCA
GTTGTCGACGCTCGAACTGCAGCAGGAAGTCGCGATGGCCATCGCGCAGAATCCGCTGCTCGAGAACGACGACGAATGGA
TCGCGAGTCCGCTGCGTGTCGCGGCGGACGGATCGCTGATCGCGCAGACGCCTCCCGCACAGAGCACCGAATCGTCCGTC
GGGAACAGCAGCAGCCAGTCGAGCGATCGCGCCGAGCGCGACGAACCGGCTGCCGCCGACGAATACGACGGATACGCATC
GAGCGACGCCGGCGACGGCCCGCAGTGGAATCTCGACGAATTTGGCCGCTCGAGCGGCGCGTCGGACGACGACGACCTCC
CGCCGCTGCAGGTGCAGGAAGCCGCGACGTCGCTGCGCGACCACCTGTCCGCGCAACTGCGCGTCACGCAGGCCAGCCCG
CGCGATCGCGCGCTCGTGATGTTCCTGATCGAATCGCTCGACGACGACGGCTACCTGACCGCCACGCTCGACGAAGTGCT
CGCCGATTTGCCGTCGGAACTGGAAATCGACTGCGACGAGATGAACGCGGCGCTCGCGCTGCTGCACAGTTTCGATCCGG
CCGGGGTCGGGGCCCGCTCGGCATCCGAATGCCTGAAGCTGCAGTTGCTGCGCCTCGATCCGTCCCCCACCCGCACGCTC
GCGCTCGAGATCGTGTCGCAGCATCTCGAACTGCTCGCCGCGCGGGACTTCACGCGGCTGCGCAAGCAGCTGAAGGCGAG
CGACGACGCGCTGCGCGACGCGCATGCGCTGATCCGCTCGCTGGAGCCGTTCCCCGGCGCCGCCTACGGCAAGGCCGAGG
CCGACTACGTCGTGCCCGACATCATCGTGAAGAAAGCCGGCCAGGGCTGGCTCGCGGAACTCAATCCGGAAGTCATGCCG
CGCCTGCGGATCAACAACCTGTACGCGAACATCCTGCGCAACAGCCGCGGCGATCCGGCGGCCGGATCGTTGAAACAGCA
GCTCCAGGAAGCACGCTGGCTGATCAAGAACATCCAGCAGCGATTCGACACGATCCTGCGCGTCGCGCAGGCTATTGTCG
AACGTCAGAAGAATTTCTTTGCGCACGGTGAAATTGCCATGCGCCCCTTGGTTTTGCGGGAAATAGCTGATACGCTGGGC
CTACACGAGTCAACTGTCAGCCGTGTGACAACTGGCAAGTACATGCTGACCCCGTTCGGGACGCTTGAATTTAAGTACTT
CTTCGGATCACACGTTTCGACCGACACCGGAGGCGCCGCATCGTCGACCGCCATCCGAGCCCTGATCAAGCAACTGATAG
GAGCTGAAGACCCAAAATCGCCACTTTCGGACAGCCGCATTGCCGAGCTGCTGGCAGAACAGGGATTCGTGGTTGCGCGC
CGCACGGTTGCCAAGTATCGCGAAGCCCTCAAGATCCCGGCAGTGAATCTGCGCAAGTCTCTTTAA

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(137-328)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(344-499)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

67.928

100

0.681

Multiple sequence alignment