Detailed information NTG0052500

Bioinformatically predicted

Overview

• Name: comFA
• Type: Machinery gene
• Locus tag: BTF1_RS23970
• Genome accession: NC_018508
• Coordinates: 4681834..4683183 (-)
• Length: 449 a.a.
• NCBI ID: WP_002083580.1
• Uniprot ID: -
• Organism: Bacillus thuringiensis HD-789
• Function: ssDNA transport into the cell (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4676834..4688183

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
BTF1_RS23950 (BTF1_24325)	secA	4677162..4679669 (-)	2508	WP_000579374.1	preprotein translocase subunit SecA	-
BTF1_RS23955 (BTF1_24330)	raiA	4679942..4680484 (-)	543	WP_000671187.1	ribosome-associated translation inhibitor RaiA	-
BTF1_RS23960 (BTF1_24335)	cspC	4680806..4681003 (-)	198	WP_001990088.1	cold shock protein CspC	-
BTF1_RS23965 (BTF1_24340)	-	4681130..4681834 (-)	705	WP_003255239.1	ComF family protein	-
BTF1_RS23970 (BTF1_24345)	comFA	4681834..4683183 (-)	1350	WP_002083580.1	ATP-dependent helicase ComFA	Machinery gene
BTF1_RS23975 (BTF1_24350)	-	4683311..4684696 (-)	1386	WP_000499483.1	C40 family peptidase	-
BTF1_RS23980 (BTF1_24355)	-	4684845..4685159 (-)	315	WP_000400857.1	helix-turn-helix domain-containing protein	-
BTF1_RS23985 (BTF1_24360)	-	4685332..4686174 (-)	843	WP_000684726.1	DegV family protein	-
BTF1_RS23990 (BTF1_24365)	-	4686411..4687046 (+)	636	WP_000926669.1	YigZ family protein	-

Sequence

Protein

• Length: 449 a.a.
• Molecular weight: 51166.05 Da
• Isoelectric Point: 10.0230

>NTDB_id=52500 BTF1_RS23970 WP_002083580.1 4681834..4683183(-) (comFA) [Bacillus thuringiensis HD-789]
MLAGKQLLLDELSSDLQRELNDLKRKGEVICVQGVKKKNSKYMCQRCGNVDQRLFASFLCRRCSKVCTYCRKCITMGRVS
ECAVLVRGIAERKREKKLNLLQWNGKLSTGQNLAAQGVVEAIKQKESFFIWAVCGAGKTEMLFYGINEALQKGERVCIAT
PRTDVVLELAPRLQEVFPYIKVAALYGGSVDKEKDAVLVVATTHQLLRYYRAFHVMVVDEIDAFPYCADQMLQYAVKQAM
KERAARIYLTATPDETWKRKFRKGEQKGVIVSGRYHRHPLPVPLFCWCGNWKKSLIHKRIPRVLLQWLQTYLNKKHPIFL
FVPHVRYIEEISLLLKSLNKRIEGVHAEDPGRKEKVAAFRKGEIPLLVTTTILERGVTVKNLQVAVLGAEEEIFSESALV
QIAGRAGRSFEAPYGEVIYFHYGKTEAMVRAKKHIQGMNKNAKEQGLID

Nucleotide

• Length: 1350 bp

>NTDB_id=52500 BTF1_RS23970 WP_002083580.1 4681834..4683183(-) (comFA) [Bacillus thuringiensis HD-789]
ATGCTGGCGGGGAAACAGTTGCTATTAGACGAACTTTCTTCAGATTTACAGAGGGAATTAAATGATTTGAAAAGGAAGGG
AGAGGTCATATGTGTACAAGGTGTAAAAAAGAAGAATTCTAAATATATGTGCCAACGCTGCGGAAATGTAGATCAGCGGC
TATTTGCGTCGTTTTTATGTAGAAGGTGCAGTAAAGTGTGCACATATTGCCGGAAGTGTATAACGATGGGGAGGGTAAGT
GAATGTGCTGTACTTGTTCGCGGGATTGCTGAAAGAAAGAGAGAAAAGAAATTAAACTTGTTACAGTGGAACGGGAAGTT
GTCTACTGGTCAGAATTTGGCGGCACAAGGTGTTGTAGAGGCTATTAAGCAAAAAGAATCATTTTTTATTTGGGCTGTAT
GCGGGGCTGGGAAAACAGAGATGTTGTTTTACGGTATTAACGAAGCGCTTCAAAAAGGAGAAAGAGTTTGTATCGCAACG
CCGAGAACGGATGTTGTTCTGGAATTAGCACCGAGATTACAAGAAGTATTTCCATATATAAAGGTAGCGGCTTTATATGG
AGGGAGTGTGGATAAAGAAAAAGATGCAGTACTAGTCGTTGCGACCACGCATCAATTATTGCGTTATTATAGGGCGTTTC
ATGTCATGGTTGTAGATGAGATAGATGCTTTTCCATATTGTGCAGATCAAATGTTACAGTACGCGGTAAAACAAGCGATG
AAAGAAAGGGCGGCGCGTATTTATTTAACTGCGACTCCAGATGAAACGTGGAAGCGAAAATTTAGAAAAGGTGAACAAAA
AGGTGTTATTGTTTCTGGACGATATCACCGTCATCCTTTGCCAGTTCCTTTATTTTGTTGGTGCGGGAATTGGAAAAAAA
GCCTCATTCATAAAAGAATTCCTCGAGTTTTACTACAGTGGTTACAAACATACTTAAATAAAAAACATCCTATTTTTTTA
TTTGTCCCCCATGTGCGATATATAGAAGAGATAAGCCTGTTGTTAAAATCATTAAACAAGCGAATTGAAGGTGTACATGC
AGAAGATCCGGGGAGAAAAGAAAAAGTAGCGGCTTTCAGAAAAGGAGAAATCCCATTATTAGTTACAACGACAATTTTAG
AGCGAGGCGTAACGGTGAAAAATTTGCAAGTAGCGGTTTTAGGGGCTGAAGAAGAAATATTCTCAGAAAGTGCACTCGTA
CAAATTGCGGGCCGAGCAGGGCGGAGCTTTGAAGCACCGTATGGAGAGGTCATTTATTTTCACTATGGTAAGACAGAGGC
GATGGTGCGCGCGAAAAAACATATTCAAGGTATGAATAAAAATGCCAAAGAACAAGGATTGATCGATTAA

Domains

Predicted by InterproScan.

• Type III restriction enzyme, res subunit (ResIII) (107-254)

• Helicase conserved C-terminal domain (Helicase_C) (311-408)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comFA	Bacillus subtilis subsp. subtilis str. 168	51.131	98.441	0.503
comFA	Latilactobacillus sakei subsp. sakei 23K	41.709	88.641	0.37